Journal ArticleDOI
Viability and neuronal differentiation of neural stem cells encapsulated in silk fibroin hydrogel functionalized with an IKVAV peptide
Wei Sun,Tania Incitti,Claudio Migliaresi,Claudio Migliaresi,Alessandro Quattrone,Simona Casarosa,Antonella Motta,Antonella Motta +7 more
TLDR
The silk fibroin hydrogel modified by IKVAV peptide showed increased cell viability and an enhanced neuronal differentiation capability, which contributed to understanding the effects of IKVsav peptide on the behaviour of neural stem cells.Abstract:
Three-dimensional (3D) porous scaffolds combined with therapeutic stem cells play vital roles in tissue engineering. The adult brain has very limited regeneration ability after injuries such as trauma and stroke. In this study, injectable 3D silk fibroin-based hydrogel scaffolds with encapsulated neural stem cells were developed, aiming at supporting brain regeneration. To improve the function of the hydrogel towards neural stem cells, silk fibroin was modified by an IKVAV peptide through covalent binding. Both unmodified and modified silk fibroin hydrogels were obtained, through sonication, with mechanical stiffness comparable to that of brain tissue. Human neural stem cells were encapsulated in both hydrogels and the effects of IKVAV peptide conjugation on cell viability and neural differentiation were assessed. The silk fibroin hydrogel modified by IKVAV peptide showed increased cell viability and an enhanced neuronal differentiation capability, which contributed to understanding the effects of IKVAV peptide on the behaviour of neural stem cells. For these reasons, IKVAV-modified silk fibroin is a promising material for brain tissue engineering. Copyright © 2015 John Wiley & Sons, Ltd.read more
Citations
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Silk protein-based hydrogels: Promising advanced materials for biomedical applications
Sonia Kapoor,Subhas C. Kundu +1 more
TL;DR: This review provides overview of the advances in silk protein-based hydrogels with a primary emphasis on hydrogel of fibroin with the aim of highlighting the research that has been done in the area of silk-basedHydrogels.
Journal ArticleDOI
Current and novel polymeric biomaterials for neural tissue engineering
TL;DR: Most of neural tissue engineering applications are in pre-clinical study, in particular for use in the central nervous system, however collagen polymer conduits aimed at regeneration of peripheral nerves have already been successfully tested in clinical trials.
Journal ArticleDOI
3D Extracellular Matrix Mimics: Fundamental Concepts and Role of Materials Chemistry to Influence Stem Cell Fate
TL;DR: This review will present the fundamentals of ECM, cover the chemical and structural features of the scaffolds used to generate ECM mimics, discuss the nature of the signaling biomolecules required and exploited to generate bioresponsive cell microenvironments able to induce a specific cell fate, and highlights the syn-thetic strategies involved to create functional 3D ECm mimics.
Journal ArticleDOI
Tissue repair and regeneration with endogenous stem cells
TL;DR: Important differences in the self-regeneration abilities of non-mammalian vertebrates and mammals, including humans, are discussed, and regeneration approaches in combination with natural and synthetic biomaterials are investigated.
Journal ArticleDOI
Processing Techniques and Applications of Silk Hydrogels in Bioengineering.
TL;DR: This work reviews the several processing methods developed to prepare advanced SF hydrogel formats, emphasizing a bottom-up approach beginning with critical structural characteristics of silk proteins and their behavior under specific gelation environments.
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