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Alexandre Wohlkonig
Researcher at Vrije Universiteit Brussel
Publications - 47
Citations - 2544
Alexandre Wohlkonig is an academic researcher from Vrije Universiteit Brussel. The author has contributed to research in topics: Ethionamide & Mycobacterium tuberculosis. The author has an hindex of 19, co-authored 46 publications receiving 1975 citations. Previous affiliations of Alexandre Wohlkonig include Université libre de Bruxelles & VU University Amsterdam.
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Journal ArticleDOI
Type IIA topoisomerase inhibition by a new class of antibacterial agents.
Benjamin D. Bax,Pan F. Chan,Drake S. Eggleston,Drake S. Eggleston,Andrew P. Fosberry,Daniel R. Gentry,Fabrice Gorrec,Fabrice Gorrec,Ilaria Giordano,Michael M. Hann,Alan Joseph Hennessy,Martin Hibbs,Jianzhong Huang,Emma J. Jones,Jo J. Jones,Kristin K. Brown,Ceri J. Lewis,Earl May,Earl May,Martin R. Saunders,Onkar M. P. Singh,Claus Spitzfaden,Carol Shen,Anthony Shillings,Andrew J. Theobald,Alexandre Wohlkonig,Alexandre Wohlkonig,Neil D. Pearson,Michael N. Gwynn +28 more
TL;DR: This work provides new insights into the mechanism of topoisomerase action and a platform for structure-based drug design of a new class of antibacterial agents against a clinically proven, but conformationally flexible, enzyme class.
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A general protocol for the generation of Nanobodies for structural biology
Els Pardon,Toon Laeremans,Sarah Triest,Søren G. F. Rasmussen,Alexandre Wohlkonig,Armin Ruf,Serge Muyldermans,Wim G. J. Hol,Brian K. Kobilka,Jan Steyaert +9 more
TL;DR: A general protocol for the generation of Nanobodies to be used as crystallization chaperones for the structural investigation of diverse conformational states of flexible (membrane) proteins and complexes thereof.
Journal ArticleDOI
Structural basis of quinolone inhibition of type IIA topoisomerases and target-mediated resistance
Alexandre Wohlkonig,Pan F. Chan,Andrew P. Fosberry,Paul Homes,Jianzhong Huang,Michael Kranz,Vaughan R. Leydon,Timothy J. Miles,Neil D. Pearson,Rajika L. Perera,Anthony Shillings,Michael N. Gwynn,Benjamin D. Bax +12 more
TL;DR: A crystal structure of moxifloxacin in complex with Acinetobacter baumannii topoisomerase IV now shows the wedge-shaped quinolone stacking between base pairs at the DNA cleavage site and binding conserved residues in theDNA cleavage domain through chelation of a noncatalytic magnesium ion.
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Reversion of antibiotic resistance in Mycobacterium tuberculosis by spiroisoxazoline SMARt-420.
Nicolas Blondiaux,Martin Moune,Matthieu Desroses,Matthieu Desroses,Rosangela Frita,Marion Flipo,Vanessa Mathys,Karine Soetaert,Mehdi Kiass,Vincent Delorme,Vincent Delorme,Kamel Djaout,Vincent Trebosc,Christian Kemmer,René Wintjens,Alexandre Wohlkonig,Rudy Antoine,Ludovic Huot,David Hot,Mireia Coscolla,Mireia Coscolla,Julia Feldmann,Julia Feldmann,Sebastien Gagneux,Sebastien Gagneux,Camille Locht,Priscille Brodin,Marc Gitzinger,Benoit Deprez,Nicolas Willand,Alain R. Baulard +30 more
TL;DR: The first-of-its-kind molecule, named SMARt-420 (Small Molecule Aborting Resistance), not only fully reverses ethionamide-acquired resistance and clears ethion chloride-resistant infection in mice, it also increases the basal sensitivity of bacteria to ethionamide.
Journal ArticleDOI
Probing the N-Terminal β-Sheet Conversion in the Crystal Structure of the Human Prion Protein Bound to a Nanobody
Romany Abskharon,Gabriele Giachin,Alexandre Wohlkonig,Sameh H. Soror,Sameh H. Soror,Els Pardon,Giuseppe Legname,Jan Steyaert +7 more
TL;DR: From this structure, it appears that the palindromic motif mediates β-enrichment in the PrP(C) monomer as one of the early events in the conversion of PrP (C) intoPrP(Sc).