K
Keith Bjornson
Researcher at Pacific Biosciences
Publications - 41
Citations - 7016
Keith Bjornson is an academic researcher from Pacific Biosciences. The author has contributed to research in topics: Polymerase & Nucleic acid. The author has an hindex of 24, co-authored 41 publications receiving 6531 citations. Previous affiliations of Keith Bjornson include Howard Hughes Medical Institute & Duke University.
Papers
More filters
Journal ArticleDOI
Real-Time DNA Sequencing from Single Polymerase Molecules
John Eid,Adrian Fehr,Jeremy Gray,Khai Luong,John Lyle,Geoff Otto,Paul Peluso,David R. Rank,Primo Baybayan,Brad Bettman,Arkadiusz Bibillo,Keith Bjornson,Bidhan Chaudhuri,Fred Christians,Ronald L. Cicero,Sonya Clark,Ravindra V. Dalal,Alex DeWinter,John Dixon,Mathieu Foquet,Alfred Gaertner,Paul Hardenbol,Cheryl Heiner,Kevin Hester,David P. Holden,Gregory J. Kearns,Xiangxu Kong,Ronald Kuse,Yves Lacroix,Steven Lin,Paul Lundquist,Congcong Ma,Patrick Marks,Mark Maxham,Devon Murphy,Insil Park,Thang Pham,Michael Phillips,Joy Roy,Robert Sebra,Gene Shen,Jon M. Sorenson,Austin B. Tomaney,Kevin Travers,Mark Trulson,John Vieceli,Jeffrey Wegener,Dawn Wu,Alicia Yang,Denis Zaccarin,Peter Zhao,Frank Zhong,Jonas Korlach,Stephen Turner +53 more
TL;DR: Single-molecule, real-time sequencing data obtained from a DNA polymerase performing uninterrupted template-directed synthesis using four distinguishable fluorescently labeled deoxyribonucleoside triphosphates (dNTPs) are presented.
Book ChapterDOI
Real-time DNA sequencing from single polymerase molecules.
Jonas Korlach,Keith Bjornson,Bidhan Chaudhuri,Ronald L. Cicero,Benjamin Flusberg,Jeremy Gray,David P. Holden,Ravi Saxena,Jeffrey Wegener,Stephen Turner +9 more
TL;DR: This chapter will first outline the principle of this single-molecule, real-time (SMRT) DNA sequencing method, followed by descriptions of its underlying components and typical sequencing run conditions.
Journal ArticleDOI
Haplotyping germline and cancer genomes with high-throughput linked-read sequencing
Grace X.Y. Zheng,Billy T. Lau,Michael Schnall-Levin,Mirna Jarosz,John Bell,Christopher Hindson,Sofia Kyriazopoulou-Panagiotopoulou,Donald A. Masquelier,Landon Merrill,Jessica M. Terry,Patrice A Mudivarti,Paul Wyatt,Rajiv Bharadwaj,Anthony J. Makarewicz,Yuan Li,Phillip Belgrader,Andrew D. Price,Adam Lowe,Patrick Marks,Gerard M Vurens,Paul Hardenbol,Luz Montesclaros,Melissa Luo,Lawrence Greenfield,Alexander Wong,David E Birch,Steven W Short,Keith Bjornson,Pranav Patel,Erik S. Hopmans,Christina Wood,Sukhvinder Kaur,Glenn K. Lockwood,David Stafford,Joshua Delaney,Indira Wu,Heather Ordonez,Susan M. Grimes,Stephanie Greer,Josephine Y Lee,Kamila Belhocine,Kristina Giorda,William Haynes Heaton,Geoffrey P. McDermott,Zachary Bent,Francesca Meschi,Nikola O Kondov,Ryan Wilson,Jorge Bernate,Shawn Gauby,Alex Kindwall,Clara Bermejo,Adrian Fehr,Adrian Chan,Serge Saxonov,Kevin D. Ness,Benjamin J. Hindson,Hanlee P. Ji +57 more
TL;DR: A microfluidics-based, linked-read sequencing technology that can phase and haplotypes generated from whole-genome sequencing of a primary colorectal adenocarcinoma and cancer genomes using nanograms of input DNA is presented.
Journal ArticleDOI
Nucleotide-promoted release of hMutSalpha from heteroduplex DNA is consistent with an ATP-dependent translocation mechanism.
TL;DR: A novel mechanism for protein translocation along DNA that supports directional movement over long distances with minimal energy input is proposed and shown to reconcile the relatively large rate of movement of MutS homologs along the helix with their modest rate of ATP hydrolysis.
Journal ArticleDOI
Resolving the full spectrum of human genome variation using Linked-Reads.
Patrick Marks,Sarah T. Garcia,Alvaro Martinez Barrio,Kamila Belhocine,Jorge Bernate,Rajiv Bharadwaj,Keith Bjornson,Claudia Catalanotti,Josh Delaney,Adrian Fehr,Ian T. Fiddes,Brendan Galvin,Haynes Heaton,Jill Herschleb,Christopher Hindson,Esty Holt,Cassandra B. Jabara,Susanna Jett,Nikka Keivanfar,Sofia Kyriazopoulou-Panagiotopoulou,Monkol Lek,Bill Kengli Lin,Adam Lowe,Shazia Mahamdallie,Shamoni Maheshwari,Tony Makarewicz,Jamie L. Marshall,Francesca Meschi,Christopher J. O'Keefe,Heather Ordonez,Pranav Patel,Andrew D. Price,Ariel Royall,Elise Ruark,Sheila Seal,Michael Schnall-Levin,Preyas Shah,David Stafford,Stephen R. Williams,Indira Wu,Andrew Wei Xu,Nazneen Rahman,Daniel G. MacArthur,Daniel G. MacArthur,Deanna M. Church +44 more
TL;DR: The data presented here show that Linked-Reads provide a scalable approach for comprehensive genome analysis that is not possible using short reads alone, and allows for simultaneous detection of small and large variants from a single library.