Michel Bouvier

TL;DR: This work has used a variety of biochemical, biophysical, and cell-based methods to demonstrate the existence, functionality, and architecture of internalized receptor complexes composed of a single GPCR, β-arrestin, and G protein.

TL;DR: A bioluminescence resonance energy transfer assay that directly monitors in real time the early interactions between human GPCRs and their cognate G-protein subunits in living human cells provides a novel tool to directly probe the dynamics and selectivity of receptor-mediated, G- protein activation-deactivation cycles.

TL;DR: The role of Receptor Phosphorylation in the Desensitization Process, and the role of a Receptor-Specific Kinase in this process, is studied.

TL;DR: The properties revealed by the expression of the beta 2AR in Sf9 cells suggest two agonist-independent traits of G protein-linked receptors, i.e., that unliganded receptors are able to activate G proteins both in membrane preparations and in whole cells and that antagonists may mediate their effects not only by preventing the binding of agonists but also by decreasing the propensity of the receptor to assume an active state.