Sean P. J. Whelan

TL;DR: The principles of transcriptional regulation have been utilized to develop a new paradigm for systematic attenuation of virulence to develop live attenuated viral vaccines.

TL;DR: Compared the template properties of a subgenomic RNA that contained the authentic 5' and 3' ends of the vesicular stomatitis virus genome with those of RNAs in which the wild-type termini were engineered to extend their complementarity, it is demonstrated that the extent of terminal complementarity rather than its exact sequence is a major determinant of whether the template predominantly directs transcription or replication.

TL;DR: Data show that the predicted MTase active-site residues K1651, D1762, K1795, and E1833 within domain VI of the VSV L protein are essential for mRNA cap methylation, and a model of mRNA processing consistent with these data is presented.

TL;DR: By applying UV transcriptional mapping to engineered variants of vesicular stomatitis virus, it is discovered that, in infected cells, transcription and replication are controlled by initiation at different positions on the viral genome.

TL;DR: In this article, a prospective assessment of mRNA-based vaccine immunogenicity in 133 adults with chronic inflammatory diseases (CID) and 53 immunocompetent controls was conducted, where participants over 18 years of age were collected before initial immunization and 1-2 weeks after the second immunization.