Stephen R. Quake

TL;DR: The isolation of IgE producing B cells from the blood of individuals with food allergies is described, followed by a detailed study of their properties by single cell RNA sequencing (scRNA-seq), and splicing within B cells of all isotypes reveals polarized germline transcription of the IgE, but not IgG4, isotype.

TL;DR: I grew up during the personal computer revolution and spent a lot of time programming computers and building things that interfaced with them, and I tried to make a career at the interface of the two fields.

TL;DR: In this paper, a mouse model of Alzheimer's disease and human fetal cells harboring mutant amyloid precursor protein was used to identify an earlier targetable phenotype and demonstrate that reversing impaired intrinsic neural precursor cell self-renewal via genetic reduction of USP16, a histone modifier and critical physiological antagonist of the Polycomb Repressor Complex 1, can prevent downstream cognitive defects and decrease astrogliosis in vivo.

TL;DR: In this paper, the authors developed a computational approach to classify thousands of cells based on published data within seconds while simultaneously identifying and highlighting new cell states such as malignancies, and tested it on human glioblastoma and melanoma and obtained high accuracy and robustness.

TL;DR: A quantitative, well-calibrated statistical model of single-cell RNAseq measurement is developed from which a sensitively detected changes in gene expression noise, a measure of cellular heterogeneity, across age and many cell types and tissues.