S
Stephen R. Quake
Researcher at Stanford University
Publications - 626
Citations - 89247
Stephen R. Quake is an academic researcher from Stanford University. The author has contributed to research in topics: Transcriptome & Biology. The author has an hindex of 132, co-authored 589 publications receiving 77778 citations. Previous affiliations of Stephen R. Quake include Agency for Science, Technology and Research & Allegheny Health Network.
Papers
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Journal ArticleDOI
Digital MDA for enumeration of total nucleic acid contamination
Paul C. Blainey,Stephen R. Quake +1 more
TL;DR: dMDA is orders of magnitude more sensitive than PCR-based techniques for detection of microbial genomic DNA fragments and opens up new possibilities for the ultrasensitive quantification of DNA fragments in a wide variety of application areas using MDA chemistry and off-the-shelf hardware developed for digital PCR.
Patent
Noninvasive diagnosis of fetal aneuploidy by sequencing
TL;DR: In this paper, the authors proposed a method to achieve digital quantification of DNA (i.e., counting differences between identical sequences) using direct shotgun sequencing followed by mapping to the chromosome of origin and enumeration of fragments per chromosome.
Journal ArticleDOI
Microfluidic device reads up to four consecutive base pairs in DNA sequencing‐by‐synthesis
TL;DR: This work has developed the first fully integrated microfluidic system for DNA sequencing-by-synthesis using this chip and fluorescence detection, and has reliably sequenced up to 4 consecutive bps.
Journal ArticleDOI
Single-cell transcriptional dynamics of flavivirus infection.
TL;DR: This work applied viscRNA-Seq to monitor dengue and Zika virus infection in cultured cells and discovered extreme heterogeneity in virus abundance, exploiting this variation to identify host factors that show complex dynamics and a high degree of specificity for either virus.
Journal ArticleDOI
Lineage tracing of human B cells reveals the in vivo landscape of human antibody class switching
Felix Horns,Christopher Vollmers,Christopher Vollmers,Derek Croote,Sally Mackey,Gary E. Swan,Cornelia L. Dekker,Mark M. Davis,Stephen R. Quake,Stephen R. Quake +9 more
TL;DR: It is discovered that closely related B cells often switch to the same class, but lose coherence as somatic mutations accumulate, suggesting that class switch recombination is directed toward specific isotypes by a cell-autonomous imprinted state.