S
Stephen R. Quake
Researcher at Stanford University
Publications - 626
Citations - 89247
Stephen R. Quake is an academic researcher from Stanford University. The author has contributed to research in topics: Transcriptome & Biology. The author has an hindex of 132, co-authored 589 publications receiving 77778 citations. Previous affiliations of Stephen R. Quake include Agency for Science, Technology and Research & Allegheny Health Network.
Papers
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Journal ArticleDOI
Single-molecule measurements calibrate green fluorescent protein surface densities on transparent beads for use with 'knock-in' animals and other expression systems.
TL;DR: Methods to quantify histidine-tagged GFP molecules (His6-GFP) bound to Ni-NTA moieties on transparent beads over a density range comprising nearly four orders of magnitude (to 30000 GFP/microm2) are developed.
Journal ArticleDOI
Microfluidic-based mini-metagenomics enables discovery of novel microbial lineages from complex environmental samples
TL;DR: A microfluidic-based mini-metagenomic method is presented which offers a statistically rigorous approach to extract novel microbial genomes while preserving single-cell resolution and was used to analyze two hot spring samples from Yellowstone National Park and extracted 29 new genomes.
Book ChapterDOI
Optofluidic Cell Selection from Complex Microbial Communities for Single-Genome Analysis
TL;DR: The construction and use of instrumentation for optical trapping inside microfluidic devices to select individual cells for analysis by methods including nucleic acid sequencing is detailed.
Journal ArticleDOI
Single molecule measurement of the “speed limit” of DNA polymerase
TL;DR: In this paper, the authors used single molecule measurements to make a detailed characterization of this behavior as a function of the template's secondary structure and the sequence context, which enabled the measurement of the intrinsic "speed limit" of DNA polymerase by separating the burst synthesis rate from pausing events.
Journal ArticleDOI
A self-assembled microlensing rotational probe
James P. Brody,Stephen R. Quake +1 more
TL;DR: The technique is demonstrated by measuring the rotational diffusion constant of the microsphere in solutions of varying viscosity and the feasibility of using this probe to measure rotational motion of biological systems is discussed.