Augustana College (Illinois)

About: Augustana College (Illinois) is a education organization based out in Rock Island, Illinois, United States. It is known for research contribution in the topics: Higher education & Population. The organization has 491 authors who have published 763 publications receiving 14274 citations. The organization is also known as: Augustana College and Theological Seminary.

Recovirus NS1-2 Has Viroporin Activity That Induces Aberrant Cellular Calcium Signaling To Facilitate Virus Replication

Abstract: Enteric viruses in the Caliciviridae family cause acute gastroenteritis in humans and animals, but the cellular processes needed for virus replication and disease remain unknown. A common strategy among enteric viruses, including rotaviruses and enteroviruses, is to encode a viral ion channel (i.e., viroporin) that is targeted to the endoplasmic reticulum (ER) and disrupts host calcium (Ca2+) homeostasis. Previous reports have demonstrated genetic and functional similarities between the nonstructural proteins of caliciviruses and enteroviruses, including the calicivirus NS1-2 protein and the 2B viroporin of enteroviruses. However, it is unknown whether caliciviruses alter Ca2+ homeostasis for virus replication or whether the NS1-2 protein has viroporin activity like its enterovirus counterpart. To address these questions, we used Tulane virus (TV), a rhesus enteric calicivirus, to examine Ca2+ signaling during infection and determine whether NS1-2 has viroporin activity that disrupts Ca2+ homeostasis. We found that TV increases Ca2+ signaling during infection and that increased cytoplasmic Ca2+ levels are important for efficient replication. Further, TV NS1-2 localizes to the endoplasmic reticulum, the predominant intracellular Ca2+ store, and the NS2 region has characteristics of a viroporin domain (VPD). NS1-2 had viroporin activity in a classic bacterial functional assay and caused aberrant Ca2+ signaling when expressed in mammalian cells, but truncation of the VPD abrogated these activities. Together, our data provide new mechanistic insights into the function of the NS2 region of NS1-2 and support the premise that enteric viruses, including those within Caliciviridae, exploit host Ca2+ signaling to facilitate their replication.IMPORTANCE Tulane virus is one of many enteric caliciviruses that cause acute gastroenteritis and diarrheal disease. Globally, enteric caliciviruses affect both humans and animals and amass >65 billion dollars per year in treatment and health care-associated costs, thus imposing an enormous economic burden. Recent progress has resulted in several cultivation systems (B cells, enteroids, and zebrafish larvae) to study human noroviruses, but mechanistic insights into the viral factors and host pathways important for enteric calicivirus replication and infection are still largely lacking. Here, we used Tulane virus, a calicivirus that is biologically similar to human noroviruses and can be cultivated by conventional cell culture, to identify and functionally validate NS1-2 as an enteric calicivirus viroporin. Viroporin-mediated calcium signaling may be a broadly utilized pathway for enteric virus replication, and its existence within caliciviruses provides a novel approach to developing antivirals and comprehensive therapeutics for enteric calicivirus diarrheal disease outbreaks.

When Things Go From Bad to Worse: The Impact of Relative Contextual Extremity on Benjamin Montgomery’s Positive Leadership and Psychological Capital:

Abstract: Drawing from positive organizational behavior, psychological capital has been shown to be a beneficial resource allowing leaders to remain positive and future-oriented. While having hope, optimism,...

Frequency-domain ultrafast passive logic: NOT and XNOR gates

Abstract: Electronic Boolean logic gates, the foundation of current computation and digital information processing, are reaching final limits in processing power. The primary obstacle is energy consumption which becomes impractically large, > 0.1 fJ/bit per gate, for signal speeds just over several GHz. Unfortunately, current solutions offer either high-speed operation or low-energy consumption. We propose a design for Boolean logic that can achieve both simultaneously (high speed and low consumption), here demonstrated for NOT and XNOR gates. Our method works by passively modifying the phase relationships among the different frequencies of an input data signal to redistribute its energy into the desired logical output pattern. We experimentally demonstrate a passive NOT gate with an energy dissipation of ~1 fJ/bit at 640 Gb/s and use it as a building block for an XNOR gate. This approach is applicable to any system that can propagate coherent waves, such as electromagnetic, acoustic, plasmonic, mechanical, or quantum. Typically, Boolean logic gates have to compromise between high speed and low energy consumption which can become limiting at scale. Here, the authors demonstrate architectures for NOT and XNOR gates that enable simultaneous low power and fast operation.

A study of posttraumatic spiritual transformation and forgiveness among victims of significant interpersonal offences

Abstract: Forgiveness and spiritual transformation, both gain and decline, may follow being victimised. The purpose of this study was to investigate the role of forgiveness in posttraumatic spiritual transformation following interpersonal offences. One hundred forty-six participants who had been “significantly wronged” by another person completed measures of event-related distress, forgiveness, and spiritual transformation. Results showed that spiritual growth was positively related to the personal importance of religion. Event-related distress and unforgiveness were positively correlated with spiritual decline. Regression analyses revealed that forgiveness did not uniquely account for a significant amount of the variance in spiritual growth after controlling for demographic variables, religious and spiritual importance, and event-related distress. Rather, religious and spiritual importance accounted for a significant amount of variance in spiritual growth. Unforgiveness uniquely predicted spiritual decline. This s...