Institution
Chinese PLA General Hospital
Healthcare•Beijing, China•
About: Chinese PLA General Hospital is a healthcare organization based out in Beijing, China. It is known for research contribution in the topics: Medicine & Population. The organization has 18037 authors who have published 12349 publications receiving 184803 citations. The organization is also known as: 301 Military Hospital.
Topics: Medicine, Population, Cancer, Transplantation, Apoptosis
Papers published on a yearly basis
Papers
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TL;DR: In this paper, a DNA computation device mediated by thermophoresis is presented for detection of tumor-derived extracellular vesicles (tEVs) for non-invasive cancer diagnosis.
Abstract: Molecular profiling of tumor-derived extracellular vesicles (tEVs) holds great promise for non-invasive cancer diagnosis. However, sensitive and accurate identification of tEVs is challenged by the heterogeneity of EV phenotypes which reflect different cell origins. Here we present a DNA computation device mediated by thermophoresis for detection of tEVs. The strategy leverages the aptamer-based logic gate using multiple protein biomarkers on single EVs as the input and thermophoretic accumulation to amplify the output signals for highly sensitive and specific profiling of tEVs. Employing this platform, we demonstrate a high accuracy of 97% for discrimination of breast cancer (BC) patients and healthy donors in a clinical cohort (n = 30). Furthermore, molecular phenotyping assessed by tEVs is in concordance with the results from tissue biopsy in BC patients. The thermophoresis-mediated molecular computation on EVs thus provides new opportunities for accurate detection and classification of cancers.
91 citations
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TL;DR: Treatment with MSCs can alleviate sepsis-associated AKI and improve survival in mice with polymicrobial sepsi, and these effects may be mediated by the inhibition of IL-17 secretion and balance of the proinflammatory and anti-inflammatory states.
Abstract: Objective: Significant progress has been made in critical care medicine during the past several decades. However, the mortality rate is still high in patients with sepsis, especially with acute kidney injury (AKI). Mesenchymal stem cells (MSCs) possess an ability to ameliorate renal injury f
91 citations
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TL;DR: It is demonstrated that adipocyte-specific overexpression of HO-1 attenuates HF-mediated adiposity and vascular dysfunction; increases insulin sensitivity; and improves adipocyte function by increasing adiponectin, Shh, and WNT10b, and by decreasing inflammatory cytokines.
Abstract: We examined the hypothesis that adipocyte dysfunction in mice fed a high-fat (HF) diet can be prevented by lentiviral-mediated and adipocyte specific-targeting delivery of the human heme oxygenase-1 (aP2-HO-1). A bolus intracardial injection of aP2-HO-1 resulted in expression of human HO-1 for up to 9.5 months. Transduction of aP2-HO-1 increased human HO-1 expression in fat tissues without affecting murine HO-1. In mice fed a HF diet, aP2-HO-1 transduction attenuated the increases in body weight, blood glucose, blood pressure, and inflammatory cytokines, as well as the content of both visceral and subcutaneous fat. Transduction of aP2-HO-1 increased the numbers of adipocytes of small cell size (P<0.05), insulin sensitivity (P<0.05), adiponectin levels, as well as vascular relaxation to acetylcholine compared with HF mice administered the aP2-green fluorescent protein. Adipocytes of mice fed a HF diet expressed high levels of peroxisome proliferator activator receptor, aP2, C/EBP, and Wnt5b proteins and displayed marked increases in Peg1/Mesoderm specific transcript (P<0.03). Transduction of aP2-HO-1 lowered the elevated levels of these proteins and increased Sonic hedgehog, Wnt10b, and β-catenin (P<0.05). Inhibition of HO activity by administration of tin mesoporphyrin to HF-fed mice transduced with the aP2-HO-1 reversed the decrease in Peg1/Mesoderm-specific transcript, TNFα, and MCP-1 levels. Collectively, this novel study demonstrates that adipocyte-specific overexpression of HO-1 attenuates HF-mediated adiposity and vascular dysfunction; increases insulin sensitivity; and improves adipocyte function by increasing adiponectin, Shh, and WNT10b, and by decreasing inflammatory cytokines. These effects are reversed by the HO activity inhibitor, stannous mesoporphyrin.
91 citations
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TL;DR: In this paper, a copper-catalyzed decarboxylative cross-coupling reaction of propiolic acids with terminal alkynes is developed leading to unsymmetric 1,3-conjugated diynes under mild conditions.
91 citations
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TL;DR: It may be a potentially effective strategy to select appropriate targets and to combine multi-antigen-targeted CAR-T cells with “OR”, “AND” and “NOT” Boolean logic gates to expand theCAR-T cell therapeutic window.
Abstract: The approval of two chimeric antigen receptor-modified T cell types by the US Food and Drug Administration (FDA) for the treatment of hematologic malignancies is a milestone in immunotherapy; however, the application of CAR-T cells has been limited by antigen escape and on-target, off-tumor toxicities. Therefore, it may be a potentially effective strategy to select appropriate targets and to combine multi-antigen-targeted CAR-T cells with “OR”, “AND” and “NOT” Boolean logic gates. We summarize the current limitations of CAR-T cells as well as the efficacy and safety of logic-gated CAR-T cells in antitumor therapy. This review will help to explore more optimized strategies to expand the CAR-T cell therapeutic window.
90 citations
Authors
Showing all 18235 results
Name | H-index | Papers | Citations |
---|---|---|---|
Jie Zhang | 178 | 4857 | 221720 |
Gregory Y.H. Lip | 169 | 3159 | 171742 |
Chao Zhang | 127 | 3119 | 84711 |
Hong Wang | 110 | 1633 | 51811 |
Shuji Ogino | 106 | 549 | 43073 |
Li Chen | 105 | 1732 | 55996 |
Jing Wang | 97 | 1123 | 53714 |
Wei Wang | 95 | 3544 | 59660 |
Zhiguo Yuan | 93 | 633 | 28645 |
Tai Hing Lam | 93 | 1168 | 51646 |
Christopher P. Crum | 87 | 412 | 32399 |
Guozhen Shen | 84 | 422 | 23992 |
Jing-Feng Li | 81 | 507 | 23434 |
Zongjin Li | 80 | 630 | 22103 |
Wan Yee Lau | 76 | 463 | 21257 |