National Ocean Service

About: National Ocean Service is a government organization based out in Silver Spring, Maryland, United States. It is known for research contribution in the topics: Algal bloom & Population. The organization has 500 authors who have published 643 publications receiving 46096 citations.

RNA-Seq analysis of seasonal and individual variation in blood transcriptomes of healthy managed bottlenose dolphins.

Abstract: The blood transcriptome can reflect both systemic exposures and pathological changes in other organs of the body because immune cells recirculate through the blood, lymphoid tissues, and affected sites. In human and veterinary medicine, blood transcriptome analysis has been used successfully to identify markers of disease or pathological conditions, but can be confounded by large seasonal changes in expression. In comparison, the use of transcriptomic based analyses in wildlife has been limited. Here we report a longitudinal study of four managed bottlenose dolphins located in Waikoloa, Hawaii, serially sampled (approximately monthly) over the course of 1 year to establish baseline information on the content and variation of the dolphin blood transcriptome. Illumina based RNA-seq analyses were carried out using both the Ensembl dolphin genome and a de novo blood transcriptome as guides. Overall, the blood transcriptome encompassed a wide array of cellular functions and processes and was relatively stable within and between animals over the course of 1 year. Principal components analysis revealed moderate clustering by sex associated with the variation among global gene expression profiles (PC1, 22 % of variance). Limited seasonal change was observed, with < 2.5 % of genes differentially expressed between winter and summer months (FDR < 0.05). Among the differentially expressed genes, cosinor analysis identified seasonal rhythmicity for the observed changes in blood gene expression, consistent with studies in humans. While the proportion of seasonally variant genes in these dolphins is much smaller than that reported in humans, the majority of those identified in dolphins were also shown to vary with season in humans. Gene co-expression network analysis identified several gene modules with significant correlation to age, sex, or hematological parameters. This longitudinal analysis of healthy managed dolphins establishes a preliminary baseline for blood transcriptome analysis in this species. Correlations with hematological parameters, distinct from muted seasonal effects, suggest that the otherwise relatively stable blood transcriptome may be a useful indicator of health and exposure. A robust database of gene expression in free-ranging and managed dolphins across seasons with known adverse health conditions or contaminant exposures will be needed to establish predictive gene expression profiles suitable for biomonitoring.

In Tide’s Way: Southeast Florida’s September 2015 Sunny-day Flood

Abstract: The Flood Event. High tides on 27 September 2015 f looded several Miami-region communities with 0.57 m of ocean water. The flooding was concerning because of the sunny-day conditions and awareness that trends of such events are accelerating within U.S. Atlantic Coast cities from rising seas (Sweet et al. 2014; Ezer and Atkinson 2014; Sweet and Marra 2016). It was the sixth largest flood measured by the National Oceanic and Atmospheric Administration (NOAA) tide gauge in Virginia Key, Florida (Miami region), since its 1994 installation (Fig. 6.1a). The five higher floods were in response to hurricanes. The f lood had substantial astronomical underpinnings (Fig. 6.1b); it occurred during spring tides and near the peak of the seasonal mean sea level (MSL), the lunar 8.8-year perigee, and the 18.6-year nodal cycles. These factors explain the 0.24-m NOAA tide prediction relative to mean higher high water (MHHW) tidal datum that delineates typical tidal inundation (Schureman 2001; Parker 2007). Yet, tide forcing alone was insufficient to produce the observed impacts as minor “nuisance” flooding begins in excess of 0.4 meters in this region (Sweet et al. 2014). Other dynamics were at play. A nontidal sea level anomaly (Fig. 6.1b, green line), which exceeded 0.15 m for a month starting September 22, reached 0.33 m during the flood and even higher for weeks afterwards. Strong high pressure over Eastern Canada (Fig. 6.1c) with >15 m s−1 northeasterlies offshore of the mid-Atlantic Bight (not shown) drove an Ekmanrelated setup along much of the U.S. East Coast. During the flood, setup was >20 cm along the southeast Florida coast as modeled by NOAA’s extratropical surge and tide operational forecast system (Funakoshi et al. 2013). Local winds, however, were calm (<3 m s−1; http://tidesandcurrents.noaa.gov/met), inverse barometer effects nonexistent (Fig. 6.1c), and dynamical wave effects minimal as inferred by the ~1 cm standard deviations during tide measurements (Sweet et al. 2015). Interestingly, Gulf Stream transport measured upstream in the Florida Current (FC) slowed to a monthly minimum of 23.4 Sverdrup (Sv; 1 Sv ≡ 106 m3 s−1) on 25 September (Fig. 6.1d), which persisted through the flood. Transport slowdowns raise MSL along the US southeast (Zhao and Johns 2014; Ezer 2016) and Florida coasts (Park and Sweet 2015) from adjustments to meridional Ekman transport (Lee and Williams 1988) and shelf-wave dynamics (Czeschel et al. 2012; Ezer 2016). Previous studies report a 0.5–1.5 cm rise in coastal MSL per 1-Sv decline in Gulf Stream system transport (Ezer et al. 2013; Woodworth et al. 2014; Goddard et al. 2015; Ezer 2016); when it slows, local tidal-flood risks increase (Sweet et al. 2009; Ezer and Atkinson 2014; Wdowinski et al. 2016). Here, we derive a contemporary return period of the flood using a time-dependent extreme value statistical model. Then, we assess the degree that (i) seasonal variability, (ii) tide cycles, (iii) FC monthly transport minimums, and (iv) a multidecadal trend have independently affected Virginia Key’s extreme water level distribution and estimate their attribution during the f lood. We conclude by analyzing how the flood’s return period changes under future SLR projections for the Miami region forced by three representative concentration pathways (RCP).

Brevetoxicosis in seabirds naturally exposed to karenia brevis blooms along the central west coast of florida

Abstract: Harmful algal bloom events caused by the dinoflagellate Karenia brevis occurred along the central west Florida, USA, coast from February 2005 through December 2005 and from August 2006 through December 2006. During these events, from 4 February 2005 through 28 November 2006, live, debilitated seabirds admitted for rehabilitation showed clinical signs that included disorientation, inability to stand, ataxia, and seizures. Testing of blood, biologic fluids, and tissues for brevetoxin by enzyme-linked immunosorbent assay found toxin present in 69% (n=95) of rehabilitating seabirds. Twelve of the 19 species of birds had evidence of brevetoxin exposure. Commonly affected species included Double-crested Cormorants (Phalacrocorax auritus), Brown Pelicans (Pelecanus occidentalis), Great Blue Herons (Ardea herodias), and Common Loons (Gavia immer). Serial blood and fecal samples taken from several live seabirds during rehabilitation showed that brevetoxin was cleared within 5–10 days after being admitted to the re...

Effects of the statin antihyperlipidemic agent simvastatin on grass shrimp, Palaemonetes pugio.

Abstract: This study investigated lethal effects (i.e., survival) and sublethal effects (glutathione, GSH; lipid peroxidation, LPx; cholesterol, CHL; and acetylcholinesterase, AChE) of the antihyperlipidemic drug simvastatin on larval and adult grass shrimp (Palaemonetes pugio). The 96-h LC50 test for larvae resulted in an estimated LC50 of 1.18 mg/L (95% confidence interval 0.98-1.42 mg/L). The adult 96-h LC50 was >10.0 mg/L. GSH and AChE levels for both the larvae and the adults were not significantly affected by simvastatin exposure. LPx levels in the larvae were significantly higher than controls in the lowest and the highest simvastatin exposures. In adult grass shrimp, LPx levels were highest in the three lowest simvastatin exposures. CHL levels were significantly reduced in larvae at the highest simvastatin exposure level of 1 mg/L while adult CHL was not affected. Both lethal and sublethal effects associated with simvastatin exposure were only observed at concentrations well above those reported in the environment.