Sharda University

About: Sharda University is a education organization based out in Greater Noida, Uttar Pradesh, India. It is known for research contribution in the topics: Computer science & Medicine. The organization has 1276 authors who have published 2012 publications receiving 16188 citations.

Low-potential immunosensor-based detection of the vascular growth factor 165 (VEGF165) using the nanocomposite platform of cobalt metal─organic framework

Abstract: The vascular endothelial growth factor 165 (VEGF165) is a quintessential biomarker in cancers. An easy and precise tool for the early detection of malignancies is required for rapid care and metastasis prevention. Cobalt-based metal–organic framework (Co-BTC-GO-MOF) nanoparticles have been used as a signal carrier for the anti-VEGF165 signaling antibody. Cobalt-based MOF was synthesized using cobalt (Co), benzene-1,3,5-tricarboxylate (BTC), and graphene oxide (GO) applying a hydrothermal method. Structure, compositions, size and morphology of the qualified sensor are determined by using distinctive analytical techniques. The Co-MOF nanoparticles are found to be thermostable, as revealed by thermal stability assay. The strategy utilises an impedimetric and differential pulse voltammetry (DPV) techniques in the presence of the [Fe(CN)6]3−/4− redox system. Compared to earlier results, this assay resulted in higher sensitivity with the limit of detection (LOD) found to be 5.23 pM in a 0.01 M buffer solution of pH 7.4 using linear scale voltammetry at room temperature. The resulting Co-BTC-GO-MOF immunosensor shows high responsiveness and selectivity in detecting VEGF165 in real-time serum samples of cancer patients. The electrochemical performance studies confirm that the intended proposed immunosensor could pave the way for the future advancement of high-performance, sensitive, reproducible and robust immunosensors for the cost-effective and initial phase detection of cancer in the future.

Evidence for a tricritical point coinciding with the triple point in (Pb 0.94 Sr 0.06 )(Zr x Ti 1 − x )O 3 : A combined synchrotron x-ray diffraction, dielectric, and Landau theory study

Abstract: We present here results of high resolution synchrotron x-ray diffraction (SXRD) and dielectric studies in conjunction with Landau theory considerations on (Pb0.94Sr0.06)(ZrxTi1-x)O3 compositions in the vicinity of the morphotropic phase boundary (MPB) to find evidence for the flattening of the free energy surface at the MPB proposed in recent ab-initio studies on strongly piezoelectric ceramics. SXRD results reveal that the tetragonal and pseudorhombohedral monoclinic compositions with x=0.515 and 0.550 transform directly into the cubic paraelectric phase, whereas for 0.520

Middle East Respiratory Syndrome (MERS) Virus—Pathophysiological Axis and the Current Treatment Strategies

Abstract: Middle East respiratory syndrome (MERS) is a lethal respiratory disease with its first case reported back in 2012 (Jeddah, Saudi Arabia). It is a novel, single-stranded, positive-sense RNA beta coronavirus (MERS-CoV) that was isolated from a patient who died from a severe respiratory illness. Later, it was found that this patient was infected with MERS. MERS is endemic to countries in the Middle East regions, such as Saudi Arabia, Jordan, Qatar, Oman, Kuwait and the United Arab Emirates. It has been reported that the MERS virus originated from bats and dromedary camels, the natural hosts of MERS-CoV. The transmission of the virus to humans has been thought to be either direct or indirect. Few camel-to-human transmissions were reported earlier. However, the mode of transmission of how the virus affects humans remains unanswered. Moreover, outbreaks in either family-based or hospital-based settings were observed with high mortality rates, especially in individuals who did not receive proper management or those with underlying comorbidities, such as diabetes and renal failure. Since then, there have been numerous reports hypothesising complications in fatal cases of MERS. Over the years, various diagnostic methods, treatment strategies and preventive measures have been strategised in containing the MERS infection. Evidence from multiple sources implicated that no treatment options and vaccines have been developed in specific, for the direct management of MERS-CoV infection. Nevertheless, there are supportive measures outlined in response to symptom-related management. Health authorities should stress more on infection and prevention control measures, to ensure that MERS remains as a low-level threat to public health.

Comparative Study on Bioremediation for Oil Spills Using Microbes

Abstract: This research work was designed to compare and evaluate the capacity of microbes in bioremediating and biodegrading of hydrocarbons which are the major constituents of petroleum oil. An oil spill is a leakage from ocean-going tankers, pipelines or other oil sources. Bioremediation for oil spills is a technique that uses microbes to eliminate contamination of hydrocarbons from water and soil, thereby making them safe for aquatic and terrestrial species. Bioremediation can be used by bacterial species, fungal species (by a process called mycoremediation) and plant species (by a process called phytoremediation). Two different species—bacterial species (Pseudomonas putida) and fungal species (Penicillium chrysogenum)—were checked individually in the laboratory condition, in order to determine and test for their capabilities in bioremediation process in oil spills. The total aerobic bacterial and fungal counts and cfu result were carried out after first and second weeks of inoculation, and was found to be significant. Solvent extraction method was primarily used to determine the percentage of oil degraded. P. chrysogenum degraded 11.1% of the oil in two weeks and P. putida degraded 6.425% after two weeks; this shows P. chrysogenum was found to degrade oil better than P. putida. Gas chromatographic (GC-MS) analysis of the residual oil extracted from bacterial, fungal as well as the control set up showed marked variations in peaks representing individual hydrocarbon components being degraded and transformed. Higher degradation was obtained with P. chrysogenum having lower peaks as compared to the bacterial and the control set up. This further confirmed the result obtained earlier with the solvent extraction method. There are three methods to clean up oil spills— physical, chemical and biological (bioremediation); but bioremediation is advantageous as it is time and cost saving than physical metho. Also unlike chemical method, no foreign or toxic chemicals are added to the site. Keywords: oil spills, hydrocarbons, bioremediation, Pseudomonas putida, Penicillium chrysogenum Cite this Article Singh R, Majumdar RS. Comparative Study on Bioremediation for Oil Spills Using Microbes. Research & Reviews: A Journal of Bioinformatics . 2017; 4(1): 16–24p.