Showing papers in "Molecular and Biochemical Parasitology in 1994"

TL;DR: It is determined that protein expression of PF83/AMA-1 is restricted to late-stage schizonts with greater than 8 nuclei, and the processed 66-kDa form can become circumferentially associated with the merozoite surface at or around the time of schizont rupture and merozosite release.

TL;DR: The glycophorin B-dependent, sialic acid-dependent invasion of trypsin-treated normal erythrocytes uses a different parasite ligand, indicating two or more sIALic-dependent pathways for invasion.

TL;DR: Artemisinin appears to react covalently with heme in malaria hemozoin both in vitro and in situ, and some of the radioactivity present in the treated he Mozoin also comigrated with the adducts on TLC.

TL;DR: DNA sequence analysis of the cloned products reveals high conservation of both the exon and intron of Leishmania strains, and variation is evident in both the length and primary sequence of the non-transcribed spacers of the mini-exon genes.

TL;DR: Testing the validity of the classification of the protozoan Neospora caninum in the family Sarcocystidae by comparing the small subunit ribosomal RNA (18S rRNA) gene sequences confirms the placing and places it as a sister group to Toxoplasma gondii in the phylum Apicomplexa.

TL;DR: The cloning and characterization of a novel antigen expressed in the bradyzoite stage of Toxoplasma gondii is described and it is found that the antigen was found to be expressed abundantly in cysts but could not be detected in tachyzoites or within the parasitophorous vacuole of tachyzosite infected host cells.

TL;DR: The recombinant form of this enzyme expressed using the pGEX2T vector in Escherichia coli is active in digesting hemoglobin at acidic pH and in hydrolyzing a synthetic peptide corresponding to the putative initial cleavage site in hemoglobin.

TL;DR: The presence of RNA transcripts unique to the resistant forms of T. b.

TL;DR: Investigation of temporal expression during gametogenesis and the cellular location of the sexual stage specific protein Pfs16, a putative integral membrane protein of Plasmodium falciparum, and immunofluorescence assays and immunoelectron microscopy studies of emerged gametes indicate that in a minority of cases the parasitophorous vacuole membrane can be retained to some extent on the gamete surface.

TL;DR: A differential screening of a cDNA library yielded a clone that represents a stage specific gene whose transcripts are barely detectable in vegetatively grown trophozoites and maturing cysts, but are readily detected at the onset of cyst formation.

TL;DR: The secreted polymorphic antigen associated with merozoites (SPAM) is the first example of a P. falciparum antigen in which a repetitive sequence has features characteristic of a well-defined structural element.

TL;DR: Results imply that a major portion of parasite HMS activity and the activated HMS of the host cell are devoted to counteract the endogenously generated oxidative stress.

TL;DR: Overall the oxyanion efflux system in Leishmania shares several properties with other resistance efflux systems mediated by transporters, including the calcium channel blocker verapamil and arsenite act in synergy in cells exhibiting the effluxSystem.

TL;DR: Data suggest that MBP has the potential to opsonize the major developmental stages of Leishmania parasites, and provide a possible mechanism for the antibody-independent activation of complement on their surface.

TL;DR: The isolation of the EhADH2 protein helps define a new family of ADH enzymes that may be specific to anaerobic and facultativelyAnaerobic organisms.

TL;DR: The complete sequences of the P. vivax AMA-1 gene amplified from two patients, one patient was from Morong, Philippines and the other was examined in Brisbane but had visited Indonesia, Malaysia and Australia.

TL;DR: Circular plasmids were more efficient than linearized molecules at producing homologous recombination in this system, integrating by reciprocal crossing-over to produce a duplication of the DHFR-TS locus.

TL;DR: Secretion of histidine-tagged rMSP1(19)s using the expression system described may be an efficient and effective means of producing a properly folded immunogen for a human vaccine against the blood stages of P. falciparum.

TL;DR: An initiative was undertaken to sequence all genes of the human malaria parasite Plasmodium falciparum in an effort to gain a better understanding at the molecular level of the parasite that inflicts much suffering in the developing world.

TL;DR: Antibodies, raised in rabbits against the GST:Fcp1 fusion protein, were used in western blot analysis to examine expression in different life-cycle stages of F. hepatica, indicating stage-specific gene expression or processing of Fcp1.

TL;DR: Lightowlers et al. observed additional higher-molecular weight molecules, which differed in their masses by 8 kDa, suggesting that antigen B is expressed in both echinococcal species, although this antigen was previously described as an E. granulosus-specific antigen.

TL;DR: No specific sequences seem to be required for replication (and expression) in Leishmania, therefore precluding the isolation of origins of replication by genetic transformation.

TL;DR: Assays based on two filaria-specific recombinant clones with immunodiagnostic potential may be useful for large scale screening as an alternative to microfilaria or filarial antigen detection as a means of obtaining a rough index of filariasis endemicity in previously unstudied areas.

TL;DR: A novel Plasmodium falciparum sporozoite antigen, STARP (Sporozoite Threonine and Asparagine-Rich Protein), detected consistently on the surface of sporozoites obtained from laboratory strains and field isolates, has been identified and cloned, following a systematic approach aimed at isolating novel non-CS sporzoite surface antigens.

TL;DR: This protein is the first characterised surface peptide marker for infective stages of the Leishmania life cycle and fractionates with the parasite cell surface glycoconjugates, lipophosphoglycan and the glycoinositolphospholipids.