Journal ArticleDOI
A “Drug Sweeping” State of the TriABC Triclosan Efflux Pump from Pseudomonas aeruginosa
Lucien Fabre,Abigail T. Ntreh,Amira Yazidi,Inga V. Leus,Jon W. Weeks,Sudipta Bhattacharyya,Sudipta Bhattacharyya,Jakob Ruickoldt,Isabelle Rouiller,Isabelle Rouiller,Helen I. Zgurskaya,Jurgen Sygusch +11 more
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TLDR
It is proposed that selective substrate translocation involves conformational gating at the tunnel narrowing that, together with conformational ordering of TriA and TriB, creates an engaged state capable of mediating substrate efflux.About:
This article is published in Structure.The article was published on 2021-03-04. It has received 7 citations till now. The article focuses on the topics: Membrane fusion protein & Periplasmic space.read more
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Journal ArticleDOI
Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria
Ilyas Alav,Jessica Kobylka,Miriam S. Kuth,Klaas M. Pos,Martin Picard,Jessica M A Blair,Vassiliy N. Bavro +6 more
TL;DR: In this article, the authors summarize the recent advances in understanding of structural biology, function, and regulation of these systems, highlighting the previously undescribed role of periplasmic adaptor proteins (PAPs) in providing a common architectural scaffold across diverse families of transporters.
Journal ArticleDOI
Disinfectant resistance in bacteria: Mechanisms, spread, and resolution strategies.
TL;DR: In this article, a review focused on the resistance mechanisms of disinfectant resistant bacteria on biofilms, cell membrane permeability, efflux pumps, degradable enzymes, and disinfectant targets.
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Ever-Adapting RND Efflux Pumps in Gram-Negative Multidrug-Resistant Pathogens: A Race against Time.
Martijn Zwama,Kunihiko Nishino +1 more
TL;DR: In this article, the authors take a closer look at clinically, environmentally and laboratory-evolved Gram-negative bacterial strains and their decreased drug sensitivity as a result of mutations directly in the RND-type pumps themselves (from Escherichia coli, Salmonella-enterica, Neisseria gonorrhoeae, Pseudomonas aeruginosa, Acinetobacter baumannii and Legionella pneumophila).
Journal ArticleDOI
The Whole Is Bigger than the Sum of Its Parts: Drug Transport in the Context of Two Membranes with Active Efflux.
Valentin V. Rybenkov,Helen I. Zgurskaya,Chhandosee Ganguly,Inga V. Leus,Zhen Zhang,Mohammad Moniruzzaman +5 more
TL;DR: In this paper, the authors present a review of key experimental and computational approaches to the investigation of transport by individual translocators and in whole cells, summarizes key findings from these studies and outlines implications for antibiotic discovery.
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Multidrug Efflux Pumps and the Two-Faced Janus of Substrates and Inhibitors.
TL;DR: In this article, the authors discuss intriguing relationships between substrates and inhibitors of efflux pumps, as these two types of ligands face similar barriers and binding sites in the transporters and accessory proteins and both types of activities often occur with the same chemical scaffold.
References
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Journal ArticleDOI
Non-equivalent roles of two periplasmic subunits in the function and assembly of triclosan pump TriABC from Pseudomonas aeruginosa
TL;DR: It is found that analogous mutations in the α‐helical hairpin and membrane proximal domains of TriA and TriB differentially affect triclosan efflux and assembly of the complex.
Journal ArticleDOI
Opening the Channel: the Two Functional Interfaces of Pseudomonas aeruginosa OpmH with the Triclosan Efflux Pump TriABC
TL;DR: The results suggest a mechanism for functional integration of drug efflux by the inner membrane transporter and opening of the channel for transport across the outer membrane.