Journal ArticleDOI
A “Drug Sweeping” State of the TriABC Triclosan Efflux Pump from Pseudomonas aeruginosa
Lucien Fabre,Abigail T. Ntreh,Amira Yazidi,Inga V. Leus,Jon W. Weeks,Sudipta Bhattacharyya,Sudipta Bhattacharyya,Jakob Ruickoldt,Isabelle Rouiller,Isabelle Rouiller,Helen I. Zgurskaya,Jurgen Sygusch +11 more
TLDR
It is proposed that selective substrate translocation involves conformational gating at the tunnel narrowing that, together with conformational ordering of TriA and TriB, creates an engaged state capable of mediating substrate efflux.About:
This article is published in Structure.The article was published on 2021-03-04. It has received 7 citations till now. The article focuses on the topics: Membrane fusion protein & Periplasmic space.read more
Citations
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Journal ArticleDOI
Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria
Ilyas Alav,Jessica Kobylka,Miriam S. Kuth,Klaas M. Pos,Martin Picard,Jessica M A Blair,Vassiliy N. Bavro +6 more
TL;DR: In this article, the authors summarize the recent advances in understanding of structural biology, function, and regulation of these systems, highlighting the previously undescribed role of periplasmic adaptor proteins (PAPs) in providing a common architectural scaffold across diverse families of transporters.
Journal ArticleDOI
Disinfectant resistance in bacteria: Mechanisms, spread, and resolution strategies.
TL;DR: In this article, a review focused on the resistance mechanisms of disinfectant resistant bacteria on biofilms, cell membrane permeability, efflux pumps, degradable enzymes, and disinfectant targets.
Journal ArticleDOI
Ever-Adapting RND Efflux Pumps in Gram-Negative Multidrug-Resistant Pathogens: A Race against Time.
Martijn Zwama,Kunihiko Nishino +1 more
TL;DR: In this article, the authors take a closer look at clinically, environmentally and laboratory-evolved Gram-negative bacterial strains and their decreased drug sensitivity as a result of mutations directly in the RND-type pumps themselves (from Escherichia coli, Salmonella-enterica, Neisseria gonorrhoeae, Pseudomonas aeruginosa, Acinetobacter baumannii and Legionella pneumophila).
Journal ArticleDOI
The Whole Is Bigger than the Sum of Its Parts: Drug Transport in the Context of Two Membranes with Active Efflux.
Valentin V. Rybenkov,Helen I. Zgurskaya,Chhandosee Ganguly,Inga V. Leus,Zhen Zhang,Mohammad Moniruzzaman +5 more
TL;DR: In this paper, the authors present a review of key experimental and computational approaches to the investigation of transport by individual translocators and in whole cells, summarizes key findings from these studies and outlines implications for antibiotic discovery.
Journal ArticleDOI
Multidrug Efflux Pumps and the Two-Faced Janus of Substrates and Inhibitors.
TL;DR: In this article, the authors discuss intriguing relationships between substrates and inhibitors of efflux pumps, as these two types of ligands face similar barriers and binding sites in the transporters and accessory proteins and both types of activities often occur with the same chemical scaffold.
References
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RELION: implementation of a Bayesian approach to cryo-EM structure determination.
TL;DR: Developments that reduce the computational costs of the underlying maximum a posteriori (MAP) algorithm, as well as statistical considerations that yield new insights into the accuracy with which the relative orientations of individual particles may be determined are described.
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CTFFIND4: Fast and accurate defocus estimation from electron micrographs.
Alexis Rohou,Nikolaus Grigorieff +1 more
TL;DR: Modifications to the CTFFIND algorithm are described which make it significantly faster and more suitable for use with images collected using modern technologies such as dose fractionation and phase plates.
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EMAN2: an extensible image processing suite for electron microscopy.
Guang Tang,Liwei Peng,Philip R. Baldwin,Deepinder S. Mann,Wen Jiang,I. Rees,Steven J. Ludtke +6 more
TL;DR: EMAN2 has been under development for the last two years, with a completely refactored image processing library, and a wide range of features to make it much more flexible and extensible than EMAN1.
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TM-align: a protein structure alignment algorithm based on the TM-score.
Yang Zhang,Jeffrey Skolnick +1 more
TL;DR: There exists a significant correlation between the correctness of the predicted structure and the structural similarity of the model to the other proteins in the PDB, which could be used to assist in model selection in blind protein structure predictions.
Journal ArticleDOI
Scoring function for automated assessment of protein structure template quality
Yang Zhang,Jeffrey Skolnick +1 more
TL;DR: A new scoring function, the template modeling score (TM‐score), to assess the quality of protein structure templates and predicted full‐length models by extending the approaches used in Global Distance Test (GDT) 1 and MaxSub, which suggests that the TM‐score is a useful complement to the fully automated assessment ofprotein structure predictions.