A transcription factor response element for gene expression during circadian night

TLDR: The role of the Rev-ErbA/ROR response element in gene expression during circadian night is demonstrated, which is in phase with Bmal1 and in antiphase to Per2 oscillations.

Abstract: evoked by square pulse stimuli (0.066 Hz, 5‐12 mA, 200 ms) delivered by means of bipolar tungsten electrodes positioned within the lateral amygdala close to the external capsule. Population spikes were recorded in the basolateral amygdala close to lateral amygdala using glass microelectrodes (2‐3 MQ) filled with artificial cerebrospinal fluid (ACSF) 29 . HFS (five trains at 100 Hz for 1 s, 10-s interstimulus interval) was applied to induce LTP, and LFS1 (900 pulses at 1 Hz) was applied to induce LTD. Whole-cell GABA-mediated currents were isolated by adding NBQX (0.005 mM) and D-(-)-2-amino-5phosphopentanoic acid (AP5; 0.05 mM) to ACSF (bubbled with 95% O2/5% CO2 ;p H 7.3), and were recorded from visually identified somata of principal neurons of the basolateral amygdala 30 by glass electrodes (4.5‐5 MQ) 16 containing (in mM): Mg-ATP 2, CsCH3SO3 100, CsCl 60, EGTA 0.2, HEPES 10, MgCl2 1, QX314 5 and Na3GTP 0.3 (pH 7.3). Patch clamp experiments were performed at 24 ^ 1 8C at a holding potential of 270 mV. LTDi was induced by 100 stimuli at 1 Hz (LFS 2). PPF was induced as described 30 . Data are expressed as means ^ s.e.m. We tested significance using the Student’s t-test.