Journal ArticleDOI
Activation of human B lymphocytes through CD40 and interleukin 4.
TLDR
A new CD40 monoclonal antibody is produced and characterized, mAb 89, which in the presence of anti‐IgM antibodies co‐stimulates to induce B cell proliferation, and the activating properties of anti-CD40 are likely to explain its co-stimulatory effect on B cells.Abstract:
We have produced and characterized a new CD40 monoclonal antibody, mAb 89, which in the presence of anti-IgM antibodies co-stimulates to induce B cell proliferation. mAb 89 activates resting B cells as shown by an increase in cell volume and an enhanced subsequent proliferation of B cells in response to anti-IgM antibody. However, mAb 89 does not prepare B cells to respond to the growth-promoting activity of interleukin (IL) 2 or IL 4. Unlike IL 2 and IL 4, mAb 89 only weakly stimulates the proliferation of anti-IgM pre-activated B cells. Thus, the activating properties of anti-CD40 are likely to explain its co-stimulatory effect on B cells. Interestingly, the anti-CD40 mAb 89 was found to act in synergy with IL 4, but not with IL 2, in co-stimulation and restimulation assays. In this respect, anti-CD40 does not induce a significant increase of B cell surface IL 4 receptors while IL 4, but not IL 2, induces a twofold increase of the CD40 antigen expression. Thus the synergistic interaction between IL 4 and anti-CD40 may be related to the IL 4-dependent increase of CD40 antigen expression.read more
Citations
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Journal ArticleDOI
Activation of human dendritic cells through CD40 cross-linking.
Christophe Caux,Catherine Massacrier,Béatrice Vanbervliet,Bertrand Dubois,C. van Kooten,Isabelle Durand,Jacques Banchereau +6 more
TL;DR: It is demonstrated that dendritic Langerhans cells (D-Lc) generated by culturing cord blood CD34+ progenitor cells with granulocyte/macrophage colony-stimulating and tumor necrosis factor alpha (TNF-alpha) express functional CD40 at a density higher than that found on B cells.
Journal ArticleDOI
The CD40 Antigen and its Ligand
Jacques Banchereau,F Bazan,D Blanchard,Francine Brière,Jean-Pierre Galizzi,C. van Kooten,Yong-Jun Liu,Françoise Rousset,Sem Saeland +8 more
TL;DR: As other members of the tumor necrosis factor receptor family have been shown to bind several ligands, it is possible that CD40 may bind other ligands that may trigger CD40 on different cell types such as hematopoietic cells or epithelial cells.
Journal ArticleDOI
The immune responses in CD40-deficient mice: Impaired immunoglobulin class switching and germinal center formation
Tsutomu Kawabe,Tetsuji Naka,Kanji Yoshida,Takashi Tanaka,Hiroshi Fujiwara,Sachiko Suematsu,Nobuaki Yoshida,Tadamitsu Kishimoto,Hitoshi Kikutani +8 more
TL;DR: It is suggested that CD40 is essential for T cell-dependent immunoglobulin class switching and germinal center formation, but not for in vivo Tcell-dependent IgM responses and T cell -independent antibody responses.
Journal ArticleDOI
Molecular and biological characterization of a murine ligand for CD40
Richard J. Armitage,William C. Fanslow,Laura Strockbine,Timothy A. Sato,Ky N. Clifford,Brian M. Macduff,Dirk M. Anderson,Steven D. Gimpel,Terri Davis-Smith,Charles R. Maliszewski,Edward A. Clark,Craig A. Smith,Kenneth H. Grabstein,David Cosman,Melanie K. Spriggs +14 more
TL;DR: The cloning of a ligand for CD40 that is expressed on the cell surface of activated T cells and mediates B-cell proliferation in the absence of co-stimulus, as well as IgE production in the presence of interIeukin-4 is reported.
Journal ArticleDOI
CD34+ hematopoietic progenitors from human cord blood differentiate along two independent dendritic cell pathways in response to GM-CSF+TNF alpha.
Christophe Caux,Béatrice Vanbervliet,Catherine Massacrier,Colette Dezutter-Dambuyant,B de Saint-Vis,C Jacquet,K Yoneda,Sadao Imamura,Daniel Schmitt,Jacques Banchereau +9 more
TL;DR: The present study demonstrates that cord blood CD34+ HPC indeed differentiate along two independent DC pathways, and demonstrates that different pathways of DC development exist: the Langerhans cells and the CD14(+)-derived DC related to dermal DC or circulating blood DC.
References
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Journal ArticleDOI
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Journal ArticleDOI
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