Journal ArticleDOI
An "enhanced PET"-based fluorescent probe with ultrasensitivity for imaging basal and elesclomol-induced HClO in cancer cells.
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TLDR
A B ODIPY-based HClO probe (BClO) with ultrasensitivity, fast response, and high selectivity, in which the pyrrole group at the meso position has an "enhanced PET" effect on the BODIPY fluorophore.Abstract:
Reactive oxygen species (ROS) and cellular oxidant stress have long been associated with cancer. Unfortunately, the role of HClO in tumor biology is much less clear than for other ROS. Herein, we report a BODIPY-based HClO probe (BClO) with ultrasensitivity, fast response (within 1 s), and high selectivity, in which the pyrrole group at the meso position has an "enhanced PET" effect on the BODIPY fluorophore. The detection limit is as low as 0.56 nM, which is the highest sensitivity achieved to date. BClO can be facilely synthesized by a Michael addition reaction of acryloyl chloride with 2,4-dimethylpyrrole and applied to image the basal HClO in cancer cells for the first time and the time-dependent HClO generation in MCF-7 cells stimulated by elesclomol, an effective experimental ROS-generating anticancer agent.read more
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Development of Targetable Two-Photon Fluorescent Probes to Image Hypochlorous Acid in Mitochondria and Lysosome in Live Cell and Inflamed Mouse Model
Lin Yuan,Lin Yuan,Lu Wang,Bikram Keshari Agrawalla,Sungjin Park,Hai Zhu,Balasubramaniam Sivaraman,Juanjuan Peng,Qing-Hua Xu,Young-Tae Chang +9 more
TL;DR: Two-photon imaging of MITO-TP and LYSO-TP in murine model shows that higher amount of HOCl can be detected in both lysosome and mitochondria of macrophage cells during inflammation condition, suggesting these probes could not only help clarify the distribution of subcellular HOCl, but also serve as excellent tools to exploit and elucidate functions of HO Cl at sub cellular levels.
Journal ArticleDOI
Current developments in fluorescent PET (photoinduced electron transfer) sensors and switches.
TL;DR: Emphasis is placed on the molecular design and the experimental outcomes in terms of target-induced fluorescence enhancements and input/output wavelengths and the growing trends of theoretically-fortified design and intracellular application.
Intrinsic oxidative stress in cancer cells: A biochemical basis for therapeutic selectivity using ROS -generating agents
TL;DR: In this paper, the authors investigated the biochemical basis for selective anticancer activity and found that the increased oxidative stress in cancer cells forces these cells to rely more on antioxidant enzymes such as SOD for O2− elimination, making the malignant cells more vulnerable to SOD inhibition than normal cells.
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Luminescent probes for the bioimaging of small anionic species in vitro and in vivo
TL;DR: A comprehensive overview of the currently available in vitro and in vivo probes is provided and for a select range of small anions of environmental and biological relevance is provided.
Journal ArticleDOI
Design Principles, Sensing Mechanisms, and Applications of Highly Specific Fluorescent Probes for HOCl/OCl.
TL;DR: The development of new chemosensors for detection of OCl-, which operate by undergoing a chemical reaction with this ROS in conjunction with a change in emission properties, are focused on.
References
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