Journal ArticleDOI
Analysis of cell-adhesion molecule interactions using surface plasmon resonance
P A van der Merwe,A N Barclay +1 more
TLDR
Real-time optical biosensors based on surface plasmon resonance (SPR) are greatly facilitating the biochemical analysis of the molecular interactions that mediate cell adhesion, but researchers have discovered the potential pitfalls of SPR, which can lead to inaccurate affinity and kinetic measurements.About:
This article is published in Current Opinion in Immunology.The article was published on 1996-04-01. It has received 102 citations till now. The article focuses on the topics: Surface plasmon resonance & Ligand (biochemistry).read more
Citations
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Journal ArticleDOI
Use of surface plasmon resonance to probe the equilibrium and dynamic aspects of interactions between biological macromolecules.
TL;DR: The use of surface plasmon resonance biosensors to measure the binding affinities and the kinetic constants of reversible interactions between biological macromolecules is reviewed.
Journal ArticleDOI
Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum
Cécile Crosnier,Leyla Y. Bustamante,S. Josefin Bartholdson,Amy K. Bei,Michel Theron,Makoto Uchikawa,Souleymane Mboup,Omar Ndir,Dominic P. Kwiatkowski,Dominic P. Kwiatkowski,Manoj T. Duraisingh,Julian C. Rayner,Gavin J. Wright +12 more
TL;DR: The discovery of a cross-strain dependency on a single extracellular receptor–ligand pair for erythrocyte invasion by P. falciparum provides a focus for new anti-malarial therapies.
Journal ArticleDOI
Kinetic analysis of macromolecular interactions using surface plasmon resonance biosensors
TL;DR: Advances in experimental design and data analysis methods are making it possible to accurately define the assembly mechanisms and rate constants associated with macromolecular interactions.
Journal ArticleDOI
CD80 (B7-1) Binds Both CD28 and CTLA-4 with a Low Affinity and Very Fast Kinetics
TL;DR: In this paper, the authors used surface plasmon resonance to measure the affinity and kinetics of CD80 binding to CD28 and CTLA-4, and showed that these low affinities were the result of very fast dissociation rate constants (k(off)).
Journal ArticleDOI
A comparative analysis of localized and propagating surface plasmon resonance sensors: the binding of concanavalin a to a monosaccharide functionalized self-assembled monolayer.
Chanda Ranjit Yonzon,Eunhee Jeoung,Eunhee Jeoung,Shengli Zou,George C. Schatz,Milan Mrksich,Richard P. Van Duyne +6 more
TL;DR: The binding of Concanavalin A to mannose-functionalized self-assembled monolayers (SAMs) was chosen to highlight the similarities and differences between the responses of the real-time angle shift SPR and wavelength shift LSPR biosensors.
References
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Journal ArticleDOI
Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction.
TL;DR: The analysis described shows K I does not equal I 50 when competitive inhibition kinetics apply; however, K I is equal to I 50 under conditions of either noncompetitive or uncompetitive kinetics.
Journal ArticleDOI
Adhesion receptors of the immune system.
TL;DR: Three families of cell-surface molecules regulate the migration of lymphocytes and the interactions of activated cells during immune responses.
Journal ArticleDOI
Rapid measurement of binding constants and heats of binding using a new titration calorimeter.
TL;DR: A new titration calorimeter is described and results are presented for the binding of cytidine 2'-monophosphate (2'CMP) to the active site of ribonuclease A.
Journal Article
Real-time biospecific interaction analysis using surface plasmon resonance and a sensor chip technology.
Jönsson U,Lars G. Fägerstam,B Ivarsson,B Johnsson,Robert Karlsson,K Lundh,Stefan Löfås,Björn Persson,Håkan Roos,I Rönnberg +9 more
TL;DR: The development and application of a biosensor-based technology that employs surface plasmon resonance for label-free studies of molecular interactions in real time and the ability to monitor multi-molecular complexes as they form are reported on.
Journal ArticleDOI
Protein-protein interactions: methods for detection and analysis.
Eric M. Phizicky,Stanley Fields +1 more
TL;DR: This review describes how binding affinities can be evaluated by techniques including protein affinity chromatography, sedimentation, gel filtration, fluorescence methods, solid-phase sampling of equilibrium solutions, and surface plasmon resonance.