Journal ArticleDOI
Analyzing real-time PCR data by the comparative C(T) method.
Reads0
Chats0
TLDR
This protocol provides an overview of the comparative CT method for quantitative gene expression studies and various examples to present quantitative gene Expression data using this method.Abstract:
Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the PCR signal to a standard curve. Relative gene expression presents the data of the gene of interest relative to some calibrator or internal control gene. A widely used method to present relative gene expression is the comparative C(T) method also referred to as the 2 (-DeltaDeltaC(T)) method. This protocol provides an overview of the comparative C(T) method for quantitative gene expression studies. Also presented here are various examples to present quantitative gene expression data using this method.read more
Citations
More filters
Journal ArticleDOI
Benzoxazinoid Metabolites Regulate Innate Immunity against Aphids and Fungi in Maize.
S. Ahmad,Nathalie Veyrat,Ruth Gordon-Weeks,Y. Zhang,Janet L. Martin,Lesley E. Smart,Gaétan Glauser,Matthias Erb,Victor Flors,Monika Frey,Jurriaan Ton +10 more
TL;DR: DIMBOA functions as a defense regulatory signal in maize innate immunity, which acts in addition to its well-characterized activity as a biocidal defense metabolite.
Journal ArticleDOI
The Impact of Hemolysis on Cell-Free microRNA Biomarkers.
Michaela B. Kirschner,J. James B. Edelman,J. James B. Edelman,Steven Kao,Michael P. Vallely,Michael P. Vallely,Nico van Zandwijk,Glen Reid +7 more
TL;DR: The microRNA content of hemolyzed and non-hemolyzed plasma as well as RBCs was profiled to obtain a profile of microRNAs in the circulation affected or unaffected by hemolysis, highlighting the importance of rigorous quality control of plasma/serum samples used for measurement of circulating micro RNAs.
Journal ArticleDOI
Restitution of Tumor Suppressor MicroRNAs Using a Systemic Nanovector Inhibits Pancreatic Cancer Growth in Mice
Dipankar Pramanik,Nathaniel R. Campbell,Collins Karikari,Raghu R. Chivukula,Oliver A. Kent,Joshua T. Mendell,Anirban Maitra +6 more
TL;DR: The nanovector is a platform with potential broad applicability in systemic miRNA delivery to cancer cells and was confirmed in treated xenografts by significant upregulation of the corresponding miRNA and significant decreases in specific miRNA targets.
Journal ArticleDOI
Detection of High-grade Prostate Cancer Using a Urinary Molecular Biomarker–Based Risk Score
Leander Van Neste,Rianne J. Hendriks,Siebren Dijkstra,Geert Trooskens,Erik B. Cornel,Sander A. Jannink,Hans de Jong,Daphne Hessels,Frank Smit,Willem J. G. Melchers,Gisele H. J. M. Leyten,Theo M. de Reijke,Henk Vergunst,Paul J.M. Kil,Ben C. Knipscheer,Christina A. Hulsbergen-van de Kaa,Peter F.A. Mulders,Inge M. van Oort,Wim Van Criekinge,Jack A. Schalken +19 more
TL;DR: The risk score based on the mRNA liquid biopsy assay combined with traditional clinical risk factors identified men at risk of harboring high-grade PCa and resulted in a better patient risk stratification compared with current methods in clinical practice.
Journal ArticleDOI
Mitochondrial complex III is essential for suppressive function of regulatory T cells.
Samuel E. Weinberg,Benjamin D. Singer,Elizabeth M. Steinert,Carlos A. Martinez,Manan M. Mehta,Inmaculada Martínez-Reyes,Peng Gao,Kathryn A. Helmin,Hiam Abdala-Valencia,Laura A. Sena,Paul T. Schumacker,Laurence A. Turka,Navdeep S. Chandel +12 more
TL;DR: Treg cell-specific ablation of mitochondrial respiratory chain complex III in mice results in the development of fatal inflammatory disease early in life, without affecting Treg cell number.
References
More filters
Journal ArticleDOI
Analysis of relative gene expression data using real-time quantitative pcr and the 2(-delta delta c(t)) method
TL;DR: The 2-Delta Delta C(T) method as mentioned in this paper was proposed to analyze the relative changes in gene expression from real-time quantitative PCR experiments, and it has been shown to be useful in the analysis of realtime, quantitative PCR data.
Journal ArticleDOI
A new mathematical model for relative quantification in real-time RT-PCR.
TL;DR: This study enters into the particular topics of the relative quantification in real-time RT-PCR of a target gene transcript in comparison to a reference gene transcript and presents a new mathematical model that needs no calibration curve.
Journal ArticleDOI
Cluster analysis and display of genome-wide expression patterns
TL;DR: A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in pattern of gene expression, finding in the budding yeast Saccharomyces cerevisiae that clustering gene expression data groups together efficiently genes of known similar function.
Journal ArticleDOI
A Multigene Assay to Predict Recurrence of Tamoxifen-Treated, Node-Negative Breast Cancer
Soonmyung Paik,Steven Shak,Gong Tang,Chungyeul Kim,Joffre B. Baker,Maureen T. Cronin,Frederick L. Baehner,Michael G. Walker,Drew Watson,Taesung Park,William Hiller,Edwin R. Fisher,D. Lawrence Wickerham,John Bryant,Norman Wolmark +14 more
TL;DR: The recurrence score has been validated as quantifying the likelihood of distant recurrence in tamoxifen-treated patients with node-negative, estrogen-receptor-positive breast cancer and could be used as a continuous function to predict distant recurrent in individual patients.
Journal ArticleDOI
Real-time quantification of microRNAs by stem–loop RT–PCR
Caifu Chen,Dana Ridzon,Adam Broomer,Zhaohui Zhou,Danny H. Lee,Julie T. Nguyen,Maura Barbisin,Nan Lan Xu,Vikram R. Mahuvakar,Mark R. Andersen,Kaiqin Lao,Kenneth J. Livak,Karl J. Guegler +12 more
TL;DR: A novel microRNA quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis, which enables fast, accurate and sensitive miRNA expression profiling and can identify and monitor potential biomarkers specific to tissues or diseases.