Application of the CRISPR–Cas System for Efficient Genome Engineering in Plants
TLDR
It is shown that the CRISPR–Cas system can be applied to generate targeted gene mutations and gene corrections in plants, and the system can also be readily engineered to achieve deletion of large DNA fragments and for multiplex gene editing in plants.About:
This article is published in Molecular Plant.The article was published on 2013-11-01 and is currently open access. It has received 492 citations till now. The article focuses on the topics: Gene mutation & Transcription activator-like effector nuclease.read more
Citations
More filters
Journal ArticleDOI
A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants
Xingliang Ma,Qunyu Zhang,Qinlong Zhu,Liu Wei,Yan Chen,Rong Qiu,Bin Wang,Zhongfang Yang,Heying Li,Lin Yuru,Yongyao Xie,Rongxin Shen,Shuifu Chen,Zhi Wang,Yuanling Chen,Jingxin Guo,Letian Chen,Xiucai Zhao,Zhicheng Dong,Yao-Guang Liu +19 more
TL;DR: A robust CRISPR/Cas9 vector system, utilizing a plant codon optimized Cas9 gene, for convenient and high-efficiency multiplex genome editing in monocot and dicot plants and provides examples of loss-of-function gene mutations in T0 rice and Arabidopsis plants.
Journal ArticleDOI
A CRISPR/Cas9 toolkit for multiplex genome editing in plants
Hui Li Xing,Li Dong,Zhi-Ping Wang,Hai-Yan Zhang,Chun Yan Han,Bing Liu,Xue Chen Wang,Qi-Jun Chen +7 more
TL;DR: A toolkit that facilitates transient or stable expression of the CRISPR/Cas9 system in a variety of plant species, which will facilitate plant research, as it enables high efficiency generation of mutants bearing multiple gene mutations.
Journal ArticleDOI
The CRISPR/Cas9 system for plant genome editing and beyond
Luisa Bortesi,Rainer Fischer +1 more
TL;DR: The clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9 (Cas9) system is described, a recently developed tool for the introduction of site-specific double-stranded DNA breaks and the strengths and weaknesses are highlighted.
Journal ArticleDOI
Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation.
TL;DR: Comparisons of 12 combinations of eight promoters and two terminators found that the efficiency of the egg cell-specific promoter-controlled CRISPR/Cas9 system depended on the presence of a suitable terminator, and the composite promoter generated by fusing two eggcell-specific promoters resulted in much higher efficiency of mutation in the T1 generation compared with the single promoters.
Journal ArticleDOI
The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation
Hui Zhang,Jinshan Zhang,Pengliang Wei,Botao Zhang,Feng Gou,Zhengyan Feng,Yanfei Mao,Lan Yang,Heng Zhang,Nanfei Xu,Jian-Kang Zhu,Jian-Kang Zhu +11 more
TL;DR: In this paper, the CRISPR/Cas9-induced gene mutations in Arabidopsis were mostly somatic mutations in the early generation, although some mutations could be stably inherited in later generations.
References
More filters
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
TL;DR: A review of achievements made possible by site-specific nuclease technologies and applications of these reagents for genetic analysis and manipulation, including the therapeutic potential of ZFNs and TALENs, and future prospects for the field are discussed.
Journal ArticleDOI
Double-Strand Break End Resection and Repair Pathway Choice
TL;DR: The components of the end resection machinery, the role of end structure, and the cell-cycle phase on resection and the interplay of end processing with NHEJ are reviewed.