B cell differentiation and isotype switching is related to division cycle number.
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TLDR
A novel fluorescent technique reveals that T- dependent B cell activation induces cell surface changes that correlate with division cycle number and should prove useful for tracking other differentiation pathways and for future analysis of the molecular events associated with stepwise differentiation at the single cell level.Citations
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Journal ArticleDOI
Mechanism and regulation of class switch recombination.
TL;DR: Proteins required for the subsequent S-S recombination include DNA-PK, ATM, Mre11-Rad50-Nbs1, gammaH2AX, 53BP1, Mdc1, and XRCC4-ligase IV, which are important for faithful joining of S regions, and in their absence aberrant recombination and chromosomal translocations involving S regions occur.
Journal ArticleDOI
The generation of antibody-secreting plasma cells.
TL;DR: The regulation of antibody production is linked to the generation and maintenance of plasmablasts and plasma cells from their B cell precursors, and the terminal differentiation of B cells can be described as a simple probabilistic process governed by a central gene-regulatory network and modified by environmental stimuli.
Journal ArticleDOI
Helper T Cell Differentiation Is Controlled by the Cell Cycle
Jennifer J. Bird,Daniel R. Brown,Alan C. Mullen,Naomi H. Moskowitz,Michael A. Mahowald,Jenny R. Sider,Thomas F. Gajewski,Chyung Ru Wang,Steven L. Reiner +8 more
TL;DR: Examining gene expression in discrete generations of dividing cells reveals a novel mechanism governing Th cell fate that initially integrates proliferative and differentiative signals and subsequently maintains stability of the differentiated state.
Journal ArticleDOI
microRNA-155 Regulates the Generation of Immunoglobulin Class-Switched Plasma Cells
Elena Vigorito,Kerry L. Perks,Cei Abreu-Goodger,Sam F Bunting,Zou Xiang,Susan Kohlhaas,Partha Pratim Das,Eric A. Miska,Antony Rodriguez,Allan Bradley,Kenneth G. C. Smith,Cristina Rada,Anton J. Enright,Kai-Michael Toellner,Ian C. M. MacLennan,Martin R Turner +15 more
TL;DR: The intrinsic requirement for miR-155 is shown in B cell responses to thymus-dependent and -independent antigens and implicate post-transcriptional regulation of gene expression for establishing the terminal differentiation program of B cells.
Journal ArticleDOI
Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution.
TL;DR: The flow cytometric analysis of lymphocyte proliferation by serial halving of the fluorescence intensity of the vital dye CFSE can be used to determine kinetics of immune responses, track proliferation in minor subsets of cells and follow the acquisition of differentiation markers or internal proteins linked to cell division.
References
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Book ChapterDOI
CD44 and its interaction with extracellular matrix.
TL;DR: A great variety of responses has been reported to result from CD44 ligation, which indicates that downstream events following ligand binding by CD44 may vary depending on the cell type expressing CD44 and on the environment of that cell.
Journal ArticleDOI
T Cell-Dependent B Cell Activation
TL;DR: A newly identified activation antigen of helper T cells, a ligand for the B cell differentiation antigen, CD40, is a key component of contact help.
Book ChapterDOI
Mechanism and Regulation of Immunoglobulin Isotype Switching
TL;DR: This chapter focuses on the molecular mechanisms of switch recombination and other possible modes of isotype switching, especially by T cells and the cytokines they produce, as well as the regulation of isotypes switched between mice and humans.
Journal ArticleDOI
Functional and molecular characterization of single, (4-hydroxy-3-nitrophenyl)acetyl (NP)-specific, IgG1+ B cells from antibody-secreting and memory B cell pathways in the C57BL/6 immune response to NP.
TL;DR: Using multiparameter flow cytometry allows the direct and unambiguous identification and characterization of individual B cell clonotypes during their initial selection and activation in antibody responses in vivo.
Journal Article
Separation of events mediating B cell proliferation and Ig production by using T cell membranes and lymphokines.
TL;DR: Membrane-stimulated B cells did not differentiate to secrete Ig; however, addition of a lymphokine-containing supernatant from activated Th cells or the combination of IL-4 and IL-5 resulted in substantial Ig production, predominantly of the IgM, IgG1, IgA, and IgE isotypes.
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