Journal ArticleDOI
β1- and αv-class integrins cooperate to regulate myosin II during rigidity sensing of fibronectin-based microenvironments.
Herbert B. Schiller,Michaela-Rosemarie Hermann,Julien Polleux,Timothée Vignaud,Sara Zanivan,Caroline C. Friedel,Zhiqi Sun,Aurelia Raducanu,Kay-E. Gottschalk,Manuel Théry,Matthias Mann,Reinhard Fässler +11 more
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TLDR
This study assigns specific functions to distinct fibronectin-binding integrins, demonstrating that α5β1integrins accomplish force generation, whereas αv-class Integrins mediate the structural adaptations to forces, which cooperatively enable cells to sense the rigidity of fibronECTin-based microenvironments.Abstract:
How different integrins that bind to the same type of extracellular matrix protein mediate specific functions is unclear. We report the functional analysis of β1- and αv-class integrins expressed in pan-integrin-null fibroblasts seeded on fibronectin. Reconstitution with β1-class integrins promotes myosin-II-independent formation of small peripheral adhesions and cell protrusions, whereas expression of αv-class integrins induces the formation of large focal adhesions. Co-expression of both integrin classes leads to full myosin activation and traction-force development on stiff fibronectin-coated substrates, with αv-class integrins accumulating in adhesion areas exposed to high traction forces. Quantitative proteomics linked αv-class integrins to a GEF-H1-RhoA pathway coupled to the formin mDia1 but not myosin II, and α5β1 integrins to a RhoA-Rock-myosin II pathway. Our study assigns specific functions to distinct fibronectin-binding integrins, demonstrating that α5β1integrins accomplish force generation, whereas αv-class integrins mediate the structural adaptations to forces, which cooperatively enable cells to sense the rigidity of fibronectin-based microenvironments.read more
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Integrin β1 regulates marginal zone B cell differentiation and PI3K signaling
TL;DR: In this article , Andreani et al. showed that β1-integrin is also required for normal differentiation and function of marginal zone B cells, and β1integrin was shown to mediate the localization of B cells to the marginal zone of the spleen.
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Macrophage migration is differentially regulated by distinct ECM components
TL;DR: In this paper , two fibrous ECM glycoproteins, fibronectin (FN) and laminin (LAM), elicit distinct morphological and migratory responses to macrophages.
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The role of Actopaxin in tumor metastasis.
TL;DR: Actopaxin this article is a newly discovered focal adhesions (FAs) protein, actin-binding protein and pseudopodia-enriched molecule, which plays a crucial role in regulating important processes of tumor metastasis, including matrix degradation, migration, and invasion, etc.
Posted ContentDOI
Integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity
TL;DR: In this article, a highly specific integrin 5{beta}1 peptidomimetic combined with nanopatterned hydrogels is used to regulate the migration ability of basal keratinocyte sheets.
Journal ArticleDOI
SEMA7a primes integrin α5β1 engagement instructing fibroblast mechanotransduction, phenotype and transcriptional programming.
Miller Ae,Yeh Cr +1 more
TL;DR: In this article , SEMA7a regulates integrin signaling through cis-coupling with active integrin α5β1 in cis on the plasma membrane, enabling rapid integrin adhesion strengthening to fibronectin (Fn) and normal downstream mechanotransduction.
References
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MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification.
Jiirgen Cox,Matthias Mann +1 more
TL;DR: MaxQuant, an integrated suite of algorithms specifically developed for high-resolution, quantitative MS data, detects peaks, isotope clusters and stable amino acid isotope–labeled (SILAC) peptide pairs as three-dimensional objects in m/z, elution time and signal intensity space and achieves mass accuracy in the p.p.b. range.
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Integrins: Bidirectional, Allosteric Signaling Machines
TL;DR: Current structural and cell biological data suggest models for how integrins transmit signals between their extracellular ligand binding adhesion sites and their cytoplasmic domains, which link to the cytoskeleton and to signal transduction pathways.
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Universal sample preparation method for proteome analysis
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Journal ArticleDOI
Andromeda: a peptide search engine integrated into the MaxQuant environment
Jürgen Cox,Nadin Neuhauser,Annette Michalski,Richard A. Scheltema,Jesper V. Olsen,Matthias Mann +5 more
TL;DR: A novel peptide search engine using a probabilistic scoring model that can handle data with arbitrarily high fragment mass accuracy, is able to assign and score complex patterns of post-translational modifications, and accommodates extremely large databases.