Journal ArticleDOI
Biochemical characterisation of MdCXE1, a carboxylesterase from apple that is expressed during fruit ripening
Edwige J. F. Souleyre,Sean D. G. Marshall,Sean D. G. Marshall,John G. Oakeshott,Robyn J. Russell,Kim M. Plummer,Richard D. Newcomb +6 more
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The results suggest that MdCXE1 may have an impact on apple flavour through its ability to hydrolyse relevant flavour esters in ripe apple fruit.About:
This article is published in Phytochemistry.The article was published on 2011-05-01. It has received 23 citations till now. The article focuses on the topics: Malus & Ripening.read more
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Journal ArticleDOI
Biochemistry of Apple Aroma: A Review.
TL;DR: The present manuscript compiles information regarding the biosynthesis of volatile aroma compounds, including metabolic pathways, enzymes and substrates involved, factors that may affect their production and also includes a wide number of studies focused on the addition of biosynthetic precursors in their production.
Journal ArticleDOI
Completion of the canonical pathway for assembly of anticancer drugs vincristine/vinblastine in Catharanthus roseus.
TL;DR: Gene expression analyses demonstrate that both ASO and GS are highly enriched in the leaf epidermis where the pathways for catharanthine and tabersonine biosynthesis are expressed, enabling synthetic biology approaches for manufacturing a broad range of MIAs, including these dimers used in cancer treatment.
Journal ArticleDOI
The AAT1 locus is critical for the biosynthesis of esters contributing to 'ripe apple' flavour in 'Royal Gala' and 'Granny Smith' apples.
Edwige J. F. Souleyre,David Chagné,Xiuyin Chen,Sumathi Tomes,Rebecca M. Turner,Mindy Y. Wang,Ratnasiri Maddumage,Martin B. Hunt,Robert A. Winz,Claudia Wiedow,Cyril Hamiaux,Susan E. Gardiner,Daryl D. Rowan,Ross G. Atkinson +13 more
TL;DR: Results indicate that the AAT1 locus is critical for the biosynthesis of esters contributing to a 'ripe apple' flavour.
Journal ArticleDOI
Acylsugar acylhydrolases: carboxylesterase catalyzed hydrolysis of acylsugars in tomato trichomes
TL;DR: Characterization of the in vitro activities of the SpASH enzymes showed reduced activities with acylsugars produced by LA0716, presumably contributing to the high-level production of acyl sugars in the presence of highly expressed SpASH genes.
Journal ArticleDOI
Apple (Malus domestica L. Borkh) as an emerging model for fruit development
TL;DR: In this review, the available information on the regulatory aspects of apple fruit development will be reported and discussed in the light of the future perspectives of apple research.
References
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Journal ArticleDOI
Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250.
TL;DR: An improved procedure for staining of proteins following separation in polyacrylamide gels is described which utilizes the colloidal properties of Coomassie Brilliant Blue G‐250 and R‐250 to combine the advantage of much shorter staining time with high sensitivity, a clear background not requiring destaining, stepwise staining, and stable fixation after staining.
Journal ArticleDOI
The α/β hydrolase fold
David L. Ollis,Eong Cheah,Miroslaw Cygler,Bauke W. Dijkstra,Felix Frolow,Sybille M. Franken,Michal Harel,S. Jamse Remington,Israel Silman,Joseph D. Schrag,Joel L. Sussman,Koen H. G. Verschueren,Adrian Goldman +12 more
TL;DR: The alpha/beta hydrolase fold as mentioned in this paper is common to several hydrolytic enzymes of widely differing phylogenetic origin and catalytic function, including the serine protease catalytic triad.
Journal ArticleDOI
Bacterial lipolytic enzymes: classification and properties
TL;DR: An updated and extensive classification of bacterial esterases and lipases is proposed based mainly on a comparison of their amino acid sequences and some fundamental biological properties, which results in the identification of eight different families with the largest being further divided into six subfamilies.
Journal ArticleDOI
GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin
Miyako Ueguchi-Tanaka,Motoyuki Ashikari,Masatoshi Nakajima,Hironori Itoh,Etsuko Katoh,Masatomo Kobayashi,Teh-yuan Chow,Teh-yuan Chow,Yue-ie C Hsing,Hidemi Kitano,Isomaro Yamaguchi,Makoto Matsuoka +11 more
TL;DR: The results indicate that GID1 is a soluble receptor mediating GA signalling in rice, a new GA-insensitive dwarf mutant of rice, that encodes an unknown protein with similarity to the hormone-sensitive lipases.
Journal ArticleDOI
A genomics approach reveals that aroma production in apple is controlled by ethylene predominantly at the final step in each biosynthetic pathway
Robert J. Schaffer,Ellen N. Friel,Edwige J. F. Souleyre,Karen Bolitho,Kate Thodey,Susan E. Ledger,Judith H. Bowen,Jun-Hong Ma,Bhawana Nain,Daniel Cohen,Andrew P. Gleave,Ross N. Crowhurst,Bart J. Janssen,Jia-Long Yao,Richard D. Newcomb +14 more
TL;DR: This analysis suggests that the initial and final enzymatic steps with the biosynthetic pathways are important transcriptional regulation points for aroma production in apple.