Journal ArticleDOI
Biofilm Acts as a Microenvironment for Plankton-Associated Vibrio cholerae in the Aquatic Environment of Bangladesh
Mohammad Sirajul Islam,Mohammad Iqbal Kabir Jahid,Mohammad Majibur Rahman,Mohammed Ziaur Rahman,Mohammad Shafiqul Islam,Mohammad Shahidul Kabir,David A. Sack,Gary K. Schoolnik +7 more
TLDR
It is indicated that plexiglass can act as a bait to form biofilm, a microenvironment that provides shelter for plankton containing V. cholerae in the aquatic environment of Bangladesh.Abstract:
The role of biofilm as a microenvironment of plankton-associated Vibrio cholerae was investigated using plexiglass as a bait A total of 72 biofilm samples were tested using culture, direct fluorescent antibody (DFA) and molecular techniques following standard procedures. Culturable V. cholerae (smooth and rugose variants) were isolated from 33% of the samples. V. cholerae O1 were detected by FA technique throughout the year except April and June. All V. cholerae O1 isolates were positive for tcpA, ctxA and ace genes while V. cholerae non-O1, non-O139 isolates lacked these genes. V. cholerae O1 (both Inaba and Ogawa) strains had identical ribotype pattern (R1), but V. cholerae non-O1, non-O139 had different ribotype patterns. All V. cholerae O1 strains were resistant to vibrio-static compound (O/129). All V. cholerae O1 except one were resistant to trimethoprime-sulphamethoxazole, streptomycin, nalidixic acid and furazolidone but sensitive to ciprofloxacin, and tetracycline. This study indicates that plexiglass can act as a bait to form biofilm, a microenvironment that provides shelter for plankton containing V. cholerae in the aquatic environment of Bangladesh.read more
Citations
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Cholera transmission: the host, pathogen and bacteriophage dynamic
TL;DR: Advances that will help to unravel how interactions between the host, the bacterial pathogen and the lytic bacteriophage might propel and quench cholera outbreaks in endemic settings and in emergent epidemic regions such as Zimbabwe are highlighted.
Journal ArticleDOI
Living in the matrix: assembly and control of Vibrio cholerae biofilms
Jennifer K. Teschler,David Zamorano-Sánchez,Andrew S. Utada,Christopher J. A. Warner,Gerard C. L. Wong,Roger G. Linington,Fitnat H. Yildiz +6 more
TL;DR: This Review discusses V. cholerae surface attachment, various matrix components and the regulatory networks controlling biofilm formation in this model organism that spends much of its life cycle outside of the human host in the aquatic environment.
Book
A guide to the study of fresh-water biology : with special reference to aquatic insects and other invertebrate animals
James G. Needham,Paul R. Needham +1 more
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C-di-GMP Regulates Motile to Sessile Transition by Modulating MshA Pili Biogenesis and Near-Surface Motility Behavior in Vibrio cholerae.
Christopher J. Jones,Andrew S. Utada,Kimberly R. Davis,Wiriya Thongsomboon,David Zamorano Sanchez,Vinita Banakar,Lynette Cegelski,Gerard C. L. Wong,Fitnat H. Yildiz +8 more
TL;DR: The results suggest that the dynamic nature of the MshA pilus established by the assembly and disassembly of pilin subunits is essential for transition from the motile to sessile lifestyle and that c-di-GMP affects Msh a pilus assembly and function through direct interactions with the MShE ATPase.
Journal ArticleDOI
Spatial Environmental Heterogeneity Determines Young Biofilm Assemblages on Microplastics in Baltic Sea Mesocosms.
TL;DR: Results corroborate earlier findings that microplastics constitute a habitat for biofilm-forming microorganisms distinct from seston, but less from wood and suggest that vibrios are early colonizers of surfaces in general.
References
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