Journal ArticleDOI
Biologic function of the Fc fragments of E myeloma protein
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The results suggested that structural changes in the Fc portion of γE molecules initiate enzymatic sequences leading to the release of the chemical mediators in E myeloma protein PS.About:
This article is published in Immunochemistry.The article was published on 1970-08-01. It has received 146 citations till now. The article focuses on the topics: Fragment crystallizable region & Papain.read more
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Peptide blocking of IgE/receptor interaction : possibilities and pitfalls
TL;DR: Delineating the multifactorial environmental causes of this allergy pandemic is likely to take many years, and the central role of IgE antibodies in immediate hypersensitivity responses has been extensively documented since their identification as the mediator of the allergic response.
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Inhibition of mast cell sensitization in vitro by a human immunoglobulin epsilon-chain fragment synthesized in Escherichia coli.
TL;DR: The results show that a functionally active immunoglobulin molecule can be synthesized by gene cloning and expression in E. coli and makes an essential contribution to the Fc receptor binding activity of IgE.
Journal ArticleDOI
Acquisition and alteration of adhesion molecules during cultured human mast cell differentiation
Abstract: Background: Mature human mast cells express several types of adhesion molecules on their surface. Interactions between extracellular matrix (ECM) and adhesion molecules may be important for the migration and localization of mast cells and their precursors in tissues. Little is known about the regulation of adhesion molecules on mast cells during their differentiation. Objectives: To clarify the evolution of adhesion phenotype and function, we examined the expression of adhesion molecules during cultured human mast cell (CHMC) differentiation and tested adhesion of mature CHMCs to various ECM proteins. Methods: CHMCs were obtained by culturing human cord blood–derived CD34 + cells in the presence of stem cell factor and IL-6. Indirect immunofluorescence and flow cytometry was used to study cell surface expression of adhesion molecules and other markers. Mature CHMCs were tested for adhesion molecule function with immobilized matrix proteins. Results: At 1 week of culture, cells expressed CD11a, CD18, CD29, CD49d, and CD49e. At 14 weeks of culture, more mature CHMCs expressed CD11b, CD11c, CD29, CD49b, CD49c, CD49d, CD49e, CD51, CD61, and CD54 and weakly expressed CD18 and CD11a. CD11c, CD51, and CD61 appeared de novo by 4 weeks of culture, whereas CD49b and CD49c appeared by 8 weeks. CD29 decreased at 4 weeks but returned to the identical levels of 1-week-old cells by 8 weeks. Compared with levels at week 1, the levels of CD11a, CD18, CD49d, and CD49e at 4 weeks and beyond decreased during culture. Expression of CD49a, CD49f, and αd integrin was never detectable during CHMC differentiation. Fourteen-week-old CHMCs significantly adhered to the leucine-aspartic acid-valine—containing connecting segment 1 fragment of fibronectin, the 120-kd argine-glycine-aspartic acid—containing fragment of fibronectin, vitronectin, and laminin through specific integrins. Conclusion: Expression of integrins and CD54 is differentially regulated during CHMC differentiation, and mature CHMCs can adhere to many ECM proteins. These changes may facilitate emigration from the bone marrow into the circulation and ultimately contribute to the tissue homing and localization pattern seen with mature mast cells. (J Allergy Clin Immunol 2001;107:302-9.)
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CD45 exclusion- and cross-linking-based receptor signaling together broaden FcεRI reactivity.
James H. Felce,Erdinc Sezgin,Madina Wane,Heather Brouwer,Michael L. Dustin,Christian Eggeling,Simon J. Davis +6 more
TL;DR: The data suggest that a simple mechanism of CD45 exclusion–based receptor triggering could function together with cross-linking–based FcεRI signaling, broadening mast cell and basophil reactivity by enabling these cells to respond to both multivalent and surface-presented monovalent antigens.
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Structural studies of immunoglobulin E. I. Physicochemical studies of the IgE molecule.
TL;DR: This presentation summarizes available data on the physicochemical structure of IgE(PS) myeloma protein and the biological properties of this protein and structure-function relation of reaginic antibodies will be discussed by Ishizaka.
References
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Immunochemical quantitation of antigens by single radial immunodiffusion
TL;DR: By standardizing the technical conditions of the experiment it is possible to use this principle for the immunochemical determination of antigens, and the lower limit of the method was found to correspond to 0·0025 μg of antigen, and to an antigen concentrations of 1·25 μg per ml.
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Estimation of the molecular weights of proteins by Sephadex gel-filtration.
TL;DR: The results are similar to those of previous studies, where the objective was to establish a cause-and-effect relationship, rather than a straightforward relationship between the number of cells and the content of the molecule.
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