Journal ArticleDOI
Biologic function of the Fc fragments of E myeloma protein
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The results suggested that structural changes in the Fc portion of γE molecules initiate enzymatic sequences leading to the release of the chemical mediators in E myeloma protein PS.About:
This article is published in Immunochemistry.The article was published on 1970-08-01. It has received 146 citations till now. The article focuses on the topics: Fragment crystallizable region & Papain.read more
Citations
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Journal ArticleDOI
Complement-mediated cytolysis: a quick, simple method for determining levels of immunoglobulin E bound to mast cells.
B R Rosengard,D E Cochrane +1 more
Journal ArticleDOI
Purification of immunoglobulin E (IgE) antibodies from sera with high IgE titers.
Jörg Kleine-Tebbe,Robert G. Hamilton,Marianne Roebber,Lawrence M. Lichtenstein,Susan M. MacDonald +4 more
TL;DR: It is concluded that alpha hIg-MAbs are powerful tools to facilitate the affinity purification of functionally active human IgE from serum; however, when the analyte is present in low concentration, a carrier protein needs to be added to minimize non-specific loss of the material during this process.
Book ChapterDOI
Immunoglobulin E and Homocytotropic Properties
TL;DR: In this paper, the authors described the immunoglobulin E and homocytotropic properties and the minimal concentration of IgE antibody required for giving a positive P-K reaction.
Book ChapterDOI
Selective approaches to the isolation and standardization of allergens.
TL;DR: The clinical signs of type I allergic reactions seen in man and other animals, namely, local inflammation and swelling, mucous production, airways obstruc-tion, and shock are produced by release of granule-associated vasoactive media-tors such as histamine, slow-reacting substance of anaphylaxis, and other active components.
Journal ArticleDOI
Differential reduction of the inter-chain disulfide bonds of rat immunoglobulin E: Relation to biological activity
TL;DR: Reduction of most or all the inter-chain disulfide bonds in rat as in human IgE, induces changes in quaternary structure, more especially in the relationship between the Fab and Fc parts of the molecule, leading to steric blockade, by Fab, of the binding sites for mast cells present on Fc.
References
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Journal ArticleDOI
Immunochemical quantitation of antigens by single radial immunodiffusion
TL;DR: By standardizing the technical conditions of the experiment it is possible to use this principle for the immunochemical determination of antigens, and the lower limit of the method was found to correspond to 0·0025 μg of antigen, and to an antigen concentrations of 1·25 μg per ml.
Journal ArticleDOI
Estimation of the molecular weights of proteins by Sephadex gel-filtration.
TL;DR: The results are similar to those of previous studies, where the objective was to establish a cause-and-effect relationship, rather than a straightforward relationship between the number of cells and the content of the molecule.
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