Book ChapterDOI
Cannabinoid receptor CB1 and CB2 interacting proteins: Techniques, progress and perspectives.
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TLDR
A review of the current state of the literature regarding the cannabinoid receptor interactome is provided in this article, along with a commentary on the methodologies and techniques utilized, and discusses future perspectives.Abstract:
Cannabinoid receptors 1 and 2 (CB1 and CB2) are implicated in a range of physiological processes and have gained attention as promising therapeutic targets for a number of diseases. Protein-protein interactions play an integral role in modulating G protein-coupled receptor (GPCR) expression, subcellular distribution and signaling, and the identification and characterization of these will not only improve our understanding of GPCR function and biology, but may provide a novel avenue for therapeutic intervention. A variety of techniques are currently being used to investigate GPCR protein-protein interactions, including Forster/fluorescence and bioluminescence resonance energy transfer (FRET and BRET), proximity ligation assay (PLA), and bimolecular fluorescence complementation (BiFC). However, the reliable application of these methodologies is dependent on the use of appropriate controls and the consideration of the physiological context. Though not as extensively characterized as some other GPCRs, the investigation of CB1 and CB2 interacting proteins is a growing area of interest, and a range of interacting partners have been identified to date. This review summarizes the current state of the literature regarding the cannabinoid receptor interactome, provides commentary on the methodologies and techniques utilized, and discusses future perspectives.read more
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Journal ArticleDOI
Developing the Cannabinoid Receptor 2 (CB2) pharmacopoeia: past, present, and future.
TL;DR: A review of CB2 ligand development and progress in optimising physicochemical properties, understanding advanced molecular pharmacology such as functional selectivity, and clinical evaluation for CB2-targeting compounds can be found in this paper .
Journal ArticleDOI
Considerations for Cannabinoids in Perioperative Care by Anesthesiologists
Krzysztof Laudanski,Justin Wain +1 more
TL;DR: The existing formulation of cannabinoids and their biological activity are reviewed to put them into the context of the anesthesia plan execution and determine the importance of perioperative cannabinoid intake on anesthesia management.
Posted ContentDOI
Somatic and terminal CB1 receptors are differentially coupled to voltage-gated sodium channels in neocortical neurons
TL;DR: Anandamide (AEA), a major endocannabinoid, indirectly inhibits VGSC currents in neocortical neurons, providing a parallel pathway outside of nerve terminals, by which these ligands reduce neuronal excitability in the neocortex.
Journal ArticleDOI
Somatic and terminal CB1 receptors are differentially coupled to voltage-gated sodium channels in neocortical neurons
TL;DR: In this article , the authors identify a mechanism in the neocortex by which anandamide (AEA), another major endocannabinoid, but not 2-AG, powerfully inhibits somatically recorded voltage-gated sodium channel (VGSC) currents in the majority of neurons.
Journal ArticleDOI
The Role of Cannabinoid Type 2 Receptors in Parkinson’s Disease
TL;DR: A review of the current preclinical and clinical studies investigating the CB2 receptors in PD with the aim to clarify if these receptors could have a role in PD can be found in this article .
References
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Journal ArticleDOI
Expression of central and peripheral cannabinoid receptors in human immune tissues and leukocyte subpopulations.
Sylvaine Galiègue,Sophie Mary,Jean Marchand,Danielle Dussossoy,D. Carriere,Pierre Carayon,Monsif Bouaboula,David Shire,Gérard Le Fur,Pierre Casellas +9 more
TL;DR: The results suggest that CB1 and CB2 can be considered as tissue-selective antigens of the central nervous system and immune system, respectively, and cannabinoids may exert specific receptor-mediated actions on the immune system through the CB2 receptor.
Journal ArticleDOI
The MIntAct project--IntAct as a common curation platform for 11 molecular interaction databases.
Sandra Orchard,Mais G. Ammari,Bruno Aranda,Lionel Breuza,Leonardo Briganti,Fiona Broackes-Carter,Nancy H. Campbell,Gayatri Chavali,Carol Chen,Noemi del-Toro,Margaret Duesbury,Marine Dumousseau,Eugenia Galeota,Ursula Hinz,Marta Iannuccelli,Sruthi Jagannathan,Rafael C. Jimenez,Jyoti Khadake,Astrid Lagreid,Luana Licata,Ruth C. Lovering,Birgit H M Meldal,Anna N. Melidoni,Mila Milagros,Daniele Peluso,Livia Perfetto,Pablo Porras,Arathi Raghunath,Sylvie Ricard-Blum,Bernd Roechert,Andre Stutz,Michael Tognolli,Kim Van Roey,Gianni Cesareni,Henning Hermjakob +34 more
TL;DR: All data manually curated by the MINT curators have been moved into the IntAct database at EMBL-EBI and are merged with the existing IntAct dataset.
Journal ArticleDOI
The HSP90 chaperone machinery
TL;DR: Owing to the importance of HSP90 in the regulation of many cellular proteins, it has become a promising drug target for the treatment of several diseases, which include cancer and diseases associated with protein misfolding.
Journal ArticleDOI
Engrafted parenchymal brain macrophages differ from microglia in transcriptome, chromatin landscape and response to challenge
Anat Shemer,Jonathan Grozovski,Tuan Leng Tay,Jenhan Tao,Alon Volaski,Patrick Süß,Alberto Ardura-Fabregat,Mor Gross-Vered,Jung-Seok Kim,Eyal David,Louise Chappell-Maor,Lars Thielecke,Christopher K. Glass,Kerstin Cornils,Marco Prinz,Steffen Jung +15 more
TL;DR: It is established that graft-derived macrophages acquire, over time, microglia characteristics, including ramified morphology, longevity, radio-resistance and clonal expansion, however, even after prolonged CNS residence, transcriptomes and chromatin accessibility landscapes of engrafted, BM-derived Macrophages remain distinct from yolk sac-derived host microglial.