Characteristics of an SV40-plasmid recombinant and its movement into and out of the genome of a murine cell
Douglas Hanahan,Douglas Hanahan,David P. Lane,Leah Lipsich,Michael Wigler,Michael R. Botchan +5 more
TLDR
A bacterial plasmid carrying the early region of SV40 (pOT) has been stably established in high molecular weight (hmw) DNA of mouse L cells by selection for the herpes virus thymidine kinase (tk) gene as mentioned in this paper.About:
This article is published in Cell.The article was published on 1980-08-01 and is currently open access. It has received 143 citations till now. The article focuses on the topics: Plasmid & Recombinant DNA.read more
Citations
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Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
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Genetic transformation of mouse embryos by microinjection of purified DNA.
TL;DR: The results demonstrate that genes can be introduced into the mouse genome by direct insertion into the nuclei of early embryos by microinjected into pronuclei of fertilized mouse oocytes.
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Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants.
TL;DR: Statistical analysis of plasmid rescue frequencies has revealed that the MDRS loci detect differential modifications of the transgene insertions among mouse lines that show distinctive patterns of transgenes expression.
Journal Article
Hypoxia-inducible factor-1alpha is a positive factor in solid tumor growth
Heather E. Ryan,Michelle Poloni,Wayne McNulty,David A. Elson,Max Gassmann,Jeffery M. Arbeit,Randall S. Johnson +6 more
TL;DR: The evidence from these experiments indicates that hypoxic response via Hif-1α is an important positive factor in solid tumor growth and that HIF-1 α affects tumor expansion in ways unrelated to its regulation of VEGF expression.
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Stochastic Gene Expression in a Lentiviral Positive-Feedback Loop: HIV-1 Tat Fluctuations Drive Phenotypic Diversity
Leor S. Weinberger,John C. Burnett,John C. Burnett,Jared E. Toettcher,Adam P. Arkin,Adam P. Arkin,David V. Schaffer,David V. Schaffer +7 more
TL;DR: In this article, the authors present an extensive experimental/computational study of an HIV-1 model vector (LTR-GFP-IRES-Tat) and show that stochastic fluctuations in Tat influence the viral latency decision.
References
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TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
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Selective extraction of polyoma DNA from infected mouse cell cultures.
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Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.
TL;DR: The results prove the usefulness of transfection assays as a means for the bioassay and isolation of restriction fragments carrying specific genetic information and cells expressing HSV-1 tk may also provide a useful model system for the detailed analysis of eucaryotic and viral gene regulation.
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Transformation of mammalian cells with genes from procaryotes and eucaryotes
Michael Wigler,Raymond W. Sweet,Gek Kee Sim,Barbara Wold,A. Pellicer,Elizabeth Lacy,Tom Maniatis,Saul Silverstein,Richard Axel +8 more
TL;DR: This co-transformation system should allow the introduction and stable integration of virtually any defined gene into cultured cells and is likely to represent a subpopulation of competent cells which are likely to integrate other unlinked genes at frequencies higher than the general population.