Conventional methanotrophs are responsible for atmospheric methane oxidation in paddy soils.
Reads0
Chats0
TLDR
The induction of HAMO activity occurred only after the rapid growth of methanotrophic populations, and a metatranscriptome-wide association study suggests that the concurrent high- and low-affinity methane oxidation was catalysed by known meethanotrophs rather than by the proposed novel atmospheric methane oxidizers.Abstract:
Soils serve as the biological sink of the potent greenhouse gas methane with exceptionally low concentrations of ∼1.84 p.p.m.v. in the atmosphere. The as-yet-uncultivated methane-consuming bacteria have long been proposed to be responsible for this 'high-affinity' methane oxidation (HAMO). Here we show an emerging HAMO activity arising from conventional methanotrophs in paddy soil. HAMO activity was quickly induced during the low-affinity oxidation of high-concentration methane. Activity was lost gradually over 2 weeks, but could be repeatedly regained by flush-feeding the soil with elevated methane. The induction of HAMO activity occurred only after the rapid growth of methanotrophic populations, and a metatranscriptome-wide association study suggests that the concurrent high- and low-affinity methane oxidation was catalysed by known methanotrophs rather than by the proposed novel atmospheric methane oxidizers. These results provide evidence of atmospheric methane uptake in periodically drained ecosystems that are typically considered to be a source of atmospheric methane.read more
Citations
More filters
Journal ArticleDOI
A standardized and miniaturized method to investigate rhizosphere microorganisms, with a focus on methanogenic archaea and methanotrophic bacteria
TL;DR: In this article, the effects of plants and increased methane (CH4) concentrations on the abundance and activity of rhizosphere microorganisms engaged in the CH4-cycle were investigated.
Journal ArticleDOI
Functionality of methane cycling microbiome during methane flux hot moments from riparian buffer systems.
Dasiel Obregón,Tolulope G. Mafa-Attoye,Megan Baskerville,Eduardo K. Mitter,Leandro Fonseca de Souza,Maren Oelbermann,Naresh V. Thevathasan,Siu Mui Tsai,Kari E. Dunfield +8 more
TL;DR: In this article , the authors used qPCR and high-throughput amplicon sequencing from both DNA and cDNA to target methanogen and methanotroph communities.
Journal ArticleDOI
Effects of acidification on nitrification and associated nitrous oxide emission in estuarine and coastal waters
Jian Zhou,Yanling Zheng,Lijun Hou,Zhirui An,Fei Chen,Bo Liu,Li Wu,Lin Qi,Hong-Po Dong,Ping Han,Guoyu Yin,Xia Liang,Yi Yang,Xiaofei Li,Dengzhou Gao,Ye Li,Zhanfei Liu,Richard G. J. Bellerby,Min Liu +18 more
TL;DR: The authors showed that acidification can significantly decrease nitrification rate but stimulate generation of byproduct nitrous oxide (N2O) in estuarine and coastal waters, and further demonstrated that nitrifiers could significantly up-regulate gene expressions associated with intracellular pH homeostasis to cope with acidification stress.
L'hydrogène moléculaire en tant que modulateur des communautés microbiennes de sol.
TL;DR: In this article, the authors investigate the role of the hydrogene moleculaire (H2) as a source of energy ubiquitaire, retrouvee a l-etat de trace dans l’atmosphere global, mais en plus fortes concentrations dans de nombreux ecosystems tels que les terres humides and the rhizosphere des legumineuses fixatrices d’azote.
Journal ArticleDOI
A standardized and miniaturized method to investigate rhizosphere microorganisms, with a focus on methanogenic archaea and methanotrophic bacteria
TL;DR: In this paper , the effects of plants and increased methane (CH4) concentrations on the abundance and activity of rhizosphere microorganisms engaged in the CH4-cycle were investigated.
References
More filters
Journal ArticleDOI
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
TL;DR: Version 4 of MEGA software expands on the existing facilities for editing DNA sequence data from autosequencers, mining Web-databases, performing automatic and manual sequence alignment, analyzing sequence alignments to estimate evolutionary distances, inferring phylogenetic trees, and testing evolutionary hypotheses.
Journal ArticleDOI
Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
Patrick D. Schloss,Patrick D. Schloss,Sarah L. Westcott,Sarah L. Westcott,Thomas Ryabin,Justine R. Hall,Martin Hartmann,Emily B. Hollister,Ryan A. Lesniewski,Brian B. Oakley,Donovan H. Parks,Courtney J. Robinson,Jason W. Sahl,Blaz Stres,Gerhard G. Thallinger,David J. Van Horn,Carolyn F. Weber +16 more
TL;DR: M mothur is used as a case study to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the α and β diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments.
Journal ArticleDOI
Naïve Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy
TL;DR: The RDP Classifier can rapidly and accurately classify bacterial 16S rRNA sequences into the new higher-order taxonomy proposed in Bergey's Taxonomic Outline of the Prokaryotes, and the majority of the classification errors appear to be due to anomalies in the current taxonomies.
Journal ArticleDOI
UCHIME improves sensitivity and speed of chimera detection
TL;DR: UCHIME has better sensitivity than ChimeraSlayer (previously the most sensitive database method), especially with short, noisy sequences, and in testing on artificial bacterial communities with known composition, UCHIME de novo sensitivity is shown to be comparable to Perseus.
Contribution of Working Group II to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change
TL;DR: Drafting Authors: Neil Adger, Pramod Aggarwal, Shardul Agrawala, Joseph Alcamo, Abdelkader Allali, Oleg Anisimov, Nigel Arnell, Michel Boko, Osvaldo Canziani, Timothy Carter, Gino Casassa, Ulisses Confalonieri, Rex Victor Cruz, Edmundo de Alba Alcaraz, William Easterling, Christopher Field, Andreas Fischlin, Blair Fitzharris.
Related Papers (5)
Nitrite-driven anaerobic methane oxidation by oxygenic bacteria
Katharina F. Ettwig,Margaret K. Butler,Margaret K. Butler,Denis Le Paslier,Denis Le Paslier,Eric Pelletier,Eric Pelletier,Sophie Mangenot,Marcel M. M. Kuypers,Frank Schreiber,Bas E. Dutilh,Johannes Zedelius,Dirk de Beer,Jolein Gloerich,Hans J. C. T. Wessels,Theo A. van Alen,Francisca A. Luesken,Ming L. Wu,Katinka T. van de Pas-Schoonen,Huub J. M. Op den Camp,Eva M. Janssen-Megens,Kees-Jan Francoijs,Henk Stunnenberg,Jean Weissenbach,Jean Weissenbach,Mike S. M. Jetten,Marc Strous,Marc Strous +27 more
Production, oxidation, emission and consumption of methane by soils: A review
Jean Le Mer,Pierre-Armand Roger +1 more
Three decades of global methane sources and sinks
S. Kirschke,Philippe Bousquet,Philippe Ciais,Marielle Saunois,Josep G. Canadell,Edward J. Dlugokencky,Peter Bergamaschi,Daniel Bergmann,Donald R. Blake,Lori Bruhwiler,Philip Cameron-Smith,Simona Castaldi,Simona Castaldi,Frédéric Chevallier,Liang Feng,Annemarie Fraser,Martin Heimann,Elke L. Hodson,Sander Houweling,Béatrice Josse,Paul J. Fraser,Paul B. Krummel,Jean-Francois Lamarque,Ray L. Langenfelds,Corinne Le Quéré,Vaishali Naik,Simon O'Doherty,Paul I. Palmer,Isabelle Pison,David A. Plummer,Benjamin Poulter,Ronald G. Prinn,Matthew Rigby,Bruno Ringeval,Bruno Ringeval,Monia Santini,Martina Schmidt,Drew Shindell,Isobel J. Simpson,Renato Spahni,L. Paul Steele,Sarah A. Strode,Kengo Sudo,Sophie Szopa,Guido R. van der Werf,Apostolos Voulgarakis,Apostolos Voulgarakis,Michiel van Weele,Ray F. Weiss,J. E. Williams,Guang Zeng +50 more