Cytokinins are initial targets of light in the control of bud outgrowth
Hanaé Roman,Tiffanie Girault,François Barbier,Thomas Péron,Nathalie Brouard,Aleš Pěnčík,Ondřej Novák,Alain Vian,Soulaiman Sakr,Jérémy Lothier,José Le Gourrierec,Nathalie Leduc +11 more
TLDR
Molecular analysis of genes involved in major mechanisms of bud outgrowth reveal that, when supplied in darkness, CKs up-regulate their expression as rapidly and as intensely as WL, and suggest that CKs are initial components of the light signaling pathway that controls the initiation of budOutgrowth.Abstract:
Bud outgrowth is controlled by environmental and endogenous factors. Through the use of the photosynthesis inhibitor norflurazon and of masking experiments, evidence is given here that light acts mainly as a morphogenic signal in the triggering of bud outgrowth and that initial steps in the light signaling pathway involve cytokinins (CKs). Indeed, in rose (Rosa hybrida), inhibition of bud outgrowth by darkness is suppressed solely by the application of CKs. In contrast, application of sugars has a limited effect. Exposure of plants to white light (WL) induces a rapid (after 3-6 h of WL exposure) up-regulation of CK synthesis (RhIPT3 and RhIPT5), of CK activation (RhLOG8), and of CK putative transporter RhPUP5 genes and to the repression of the CK degradation RhCKX1 gene in the node. This leads to the accumulation of CKs in the node within 6 h and in the bud at 24 h and to the triggering of bud outgrowth. Molecular analysis of genes involved in major mechanisms of bud outgrowth (strigolactone signaling [RwMAX2], metabolism and transport of auxin [RhPIN1, RhYUC1, and RhTAR1], regulation of sugar sink strength [RhVI, RhSUSY, RhSUC2, and RhSWEET10], and cell division and expansion [RhEXP and RhPCNA]) reveal that, when supplied in darkness, CKs up-regulate their expression as rapidly and as intensely as WL Additionally, up-regulation of CKs by WL promotes xylem flux toward the bud, as evidenced by Methylene Blue accumulation in the bud after CK treatment in the dark. Altogether, these results suggest that CKs are initial components of the light signaling pathway that controls the initiation of bud outgrowth.read more
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An Update on the Signals Controlling Shoot Branching.
François Barbier,Elizabeth A. Dun,Stephanie C. Kerr,Tinashe G. Chabikwa,Christine A. Beveridge +4 more
TL;DR: This work has highlighted that auxin export from a bud is important for its subsequent growth, and perception of the recently discovered hormone strigolactone is now quite well understood, while the downstream targets remain largely unknown.
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Light quality in plant tissue culture: does it matter?
Diego Silva Batista,Sérgio Heitor Sousa Felipe,Tatiane Dulcineia Silva,Kamila Motta de Castro,Talita Cristina Mamedes-Rodrigues,Natane Amaral Miranda,Anyela Marcela Ríos-Ríos,Daniele Vidal Faria,Evandro Alexandre Fortini,Kristhiano Chagas,Gabriela Torres-Silva,Aloisio Xavier,Ariel D. Arencibia,Wagner Campos Otoni +13 more
TL;DR: This review presents relevant topics on the influence of light in various plant tissue culture-based techniques, as directed by the light used in the culture shelves.
Journal ArticleDOI
BRANCHED1: A Key Hub of Shoot Branching.
Ming Wang,Marie-Anne Le Moigne,Jessica Bertheloot,Laurent Crespel,Maria Dolores Perez-Garcia,Laurent Ogé,Sabine Demotes-Mainard,Latifa Hamama,Jean-Michel Davière,Soulaiman Sakr +9 more
TL;DR: This review highlights the central role of BRC1 in shoot branching and its responsiveness to different stimuli, and emphasizes the different knowledge gaps that should be addressed in the near future.
Journal ArticleDOI
Transcriptome and metabolite changes during hydrogen cyanamide-induced floral bud break in sweet cherry
Irina Alexandra Ionescu,Gregorio López-Ortega,Meike Burow,Almudena Bayo-Canha,Alexander Junge,Oliver Gericke,Birger Lindberg Møller,Raquel Sánchez-Pérez +7 more
TL;DR: This work identified the most up-regulated pathways: the cytokinin pathway, as well as the jasmonate and the hydrogen cyanide pathway, which strongly suggest an inductive effect of these metabolites in bud dormancy release and provide a stepping stone for the characterization of key genes in bud roommancy release.
Journal ArticleDOI
Hormonal Orchestration of Bud Dormancy Cycle in Deciduous Woody Perennials.
Jianyang Liu,Sherif M. Sherif +1 more
TL;DR: In this review, a recent understanding of the roles of plant hormones, their cross talks, and their potential interactions with DAM proteins during dormancy is discussed.
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