Journal ArticleDOI
Detecting microdomains in intact cell membranes.
Reads0
Chats0
TLDR
In this review, a variety of experimental techniques that have been or might be used to look for lipid microdomains in intact cell membranes are described and the strengths and limitations of different techniques for microdomain identification and characterization are assessed.Abstract:
Current models for cellular plasma membranes focus on spatial heterogeneity and how this heterogeneity relates to cell function. In particular, putative lipid raft membrane domains have been postulated to exist based in large part on the results that a significant fraction of the membrane is detergent insoluble and that molecules facilitating key membrane processes like signal transduction are often found in the detergent-resistant membrane fraction. Yet, the in vivo existence of lipid rafts remains extremely controversial because, despite being sought for more than a decade, evidence for their presence in intact cell membranes is inconclusive. In this review, a variety of experimental techniques that have been or might be used to look for lipid microdomains in intact cell membranes are described. Experimental results are highlighted and the strengths and limitations of different techniques for microdomain identification and characterization are assessed.read more
Citations
More filters
Journal ArticleDOI
Membrane lipids: where they are and how they behave.
TL;DR: How do cells apply anabolic and catabolic enzymes, translocases and transporters, plus the intrinsic physical phase behaviour of lipids and their interactions with membrane proteins, to create the unique compositions and multiple functions of their individual membranes?
Journal ArticleDOI
Revitalizing membrane rafts: new tools and insights
Kai Simons,Mathias J. Gerl +1 more
TL;DR: How the field has matured and an evolving model in which membranes are occupied by fluctuating nanoscale assemblies of sphingolipids, cholesterol and proteins that can be stabilized into platforms that are important in signalling, viral infection and membrane trafficking are presented.
Journal ArticleDOI
Lipid rafts: at a crossroad between cell biology and physics.
TL;DR: The concept of lipid rafts as it has emerged from the study of synthetic membranes with the reality of lateral heterogeneity in biological membranes is compared.
Journal ArticleDOI
Use of cyclodextrins to manipulate plasma membrane cholesterol content: evidence, misconceptions and control strategies.
Raphael Zidovetzki,Irena Levitan +1 more
TL;DR: Under conditions commonly used for cholesterol extraction, cyclodextrins may remove cholesterol from both raft and non-raft domains of the membrane as well as alter the distribution of cholesterol between plasma and intracellular membranes.
Journal ArticleDOI
The HIV lipidome: A raft with an unusual composition
Britta Brügger,Bärbel Glass,Per Haberkant,Iris Leibrecht,Felix T. Wieland,Hans-Georg Kräusslich +5 more
TL;DR: Intriguingly, the overall lipid composition of native HIV membranes resembles detergent-resistant membrane microdomains and is strikingly different from that of host cell membranes, providing strong evidence for the existence of lipid rafts in living cells.
References
More filters
Journal ArticleDOI
Atomic force microscope
TL;DR: The atomic force microscope as mentioned in this paper is a combination of the principles of the scanning tunneling microscope and the stylus profilometer, which was proposed as a method to measure forces as small as 10-18 N. As one application for this concept, they introduce a new type of microscope capable of investigating surfaces of insulators on an atomic scale.
Journal ArticleDOI
Functional rafts in cell membranes
Kai Simons,Elina Ikonen +1 more
TL;DR: A new aspect of cell membrane structure is presented, based on the dynamic clustering of sphingolipids and cholesterol to form rafts that move within the fluid bilayer that function as platforms for the attachment of proteins when membranes are moved around inside the cell and during signal transduction.
Journal ArticleDOI
Lipid rafts and signal transduction
Kai Simons,Derek Toomre +1 more
TL;DR: It is now becoming clear that lipid micro-environments on the cell surface — known as lipid rafts — also take part in this process of signalling transduction, where protein–protein interactions result in the activation of signalling cascades.
Journal ArticleDOI
Acceleration and trapping of particles by radiation pressure
TL;DR: In this paper, it is hypothesized that similar acceleration and trapping are possible with atoms and molecules using laser light tuned to specific optical transitions, and the implications for isotope separation and other applications of physical interest are discussed.
Journal ArticleDOI
Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface
Deborah A. Brown,John K. Rose +1 more
TL;DR: It is shown that a protein with a glycosylphosphatidyl inositol (GPI) anchor can be recovered from lysates of epithelial cells in a low density, detergent-insoluble form, supporting the model proposed by Simons and colleagues for sorting of certain membrane proteins to the apical surface after intracellular association with glycosphingolipids.
Related Papers (5)
Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface
Deborah A. Brown,John K. Rose +1 more