Journal ArticleDOI
Directing stem cell fate on hydrogel substrates by controlling cell geometry, matrix mechanics and adhesion ligand composition.
TLDR
A microengineering strategy to vary single cell geometry and the composition of adhesion ligands - on substrates that approximate the mechanical properties of soft tissues - to study adipogenesis and neurogenesis in adherent mesenchymal stem cells is demonstrated.About:
This article is published in Biomaterials.The article was published on 2013-11-01. It has received 241 citations till now. The article focuses on the topics: Stem cell & Cell fate determination.read more
Citations
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Journal ArticleDOI
Materials as stem cell regulators
TL;DR: Recent evidence that shows that inherent material properties may be engineered to dictate stem cell fate decisions are discussed, and a subset of the operative signal transduction mechanisms that have begun to emerge are overviewed.
Journal ArticleDOI
Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment
Guoyou Huang,Fei Li,Xin Zhao,Yufei Ma,Yuhui Li,Min Lin,Guorui Jin,Tian Jian Lu,Guy M. Genin,Feng Xu +9 more
TL;DR: This review encapsulates where recent advances appear to leave the ever-shifting state of the art in the cell microenvironment, and it highlights areas in which substantial potential and uncertainty remain.
Journal ArticleDOI
The Horizon of Materiobiology: A Perspective on Material-Guided Cell Behaviors and Tissue Engineering
Yulin Li,Yin Xiao,Changsheng Liu +2 more
TL;DR: In this article, the authors systematically summarize the recent progress on how biomaterials with controllable properties (e.g., compositional/degradable dynamics, mechanical properties, 2D topography, and 3D geometry) can regulate cell behaviors and tissue/organ functions.
The horizon of materiobiology: A perspective on material-guided cell behaviors and tissue engineering
Yulin Li,Yin Xiao,Changsheng Liu +2 more
TL;DR: This Review highlights that it is more important to develop ECM-mimicking biomaterials having a self-regenerative capacity to stimulate tissue regeneration, instead of attempting to recreate the complexity of living tissues or tissue constructs ex vivo.
Journal ArticleDOI
Matrix Stiffness and Nanoscale Spatial Organization of Cell-Adhesive Ligands Direct Stem Cell Fate
TL;DR: Both matrix stiffness and nanoscale spatial organization of cell-adhesive ligands direct stem cell fate, and it is revealed that RGD nanospacing affects spreading area and differentiation of rat MSCs, regardless of the hydrogel stiffness.
References
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Control of stem cell fate by physical interactions with the extracellular matrix.
Farshid Guilak,Daniel M. Cohen,Bradley T. Estes,Jeffrey M. Gimble,Wolfgang Liedtke,Christopher S. Chen +5 more
TL;DR: Some of the physical processes by which cues from the ECM can influence stem cell fate are reviewed, with particular relevance to the use of stem cells in tissue engineering and regenerative medicine.
Journal ArticleDOI
Geometric cues for directing the differentiation of mesenchymal stem cells.
TL;DR: The role that geometric shape cues can play in orchestrating the mechanochemical signals and paracrine/autocrine factors that can direct MSCs to appropriate fates is pointed to.
Journal ArticleDOI
Extracellular-matrix tethering regulates stem-cell fate
Britta Trappmann,Julien E. Gautrot,Julien E. Gautrot,John T. Connelly,John T. Connelly,Daniel G. T. Strange,Yuan Li,Michelle L. Oyen,Martien A. Cohen Stuart,Heike Boehm,Heike Boehm,Bojun Li,Viola Vogel,Joachim P. Spatz,Joachim P. Spatz,Fiona M. Watt,Wilhelm T. S. Huck,Wilhelm T. S. Huck +17 more
TL;DR: It is concluded that stem cells exert a mechanical force on collagen fibres and gauge the feedback to make cell-fate decisions, and are regulated by the elastic modulus of PAAm.