Diversity and Distribution of Archaea in the Mangrove Sediment of Sundarbans.
Anish Bhattacharyya,Niladri Shekhar Majumder,Pijush Basak,Shayantan Mukherji,Debojyoti Roy,Sudip Nag,Anwesha Haldar,Dhrubajyoti Chattopadhyay,Suparna Mitra,Maitree Bhattacharyya,Abhrajyoti Ghosh +10 more
TLDR
The diversity and distribution of archaea in the tropical mangrove sediments of Sundarbans using 16S rRNA gene amplicon sequencing indicated that sediment archaeal community patterns were influenced by environmental conditions.Abstract:
Mangroves are among the most diverse and productive coastal ecosystems in the tropical and subtropical regions. Environmental conditions particular to this biome make mangroves hotspots for microbial diversity, and the resident microbial communities play essential roles in maintenance of the ecosystem. Recently, there has been increasing interest to understand the composition and contribution of microorganisms in mangroves. In the present study, we have analyzed the diversity and distribution of archaea in the tropical mangrove sediments of Sundarbans using 16S rRNA gene amplicon sequencing. The extraction of DNA from sediment samples and the direct application of 16S rRNA gene amplicon sequencing resulted in approximately 142 Mb of data from three distinct mangrove areas (Godkhali, Bonnie camp, and Dhulibhashani). The taxonomic analysis revealed the dominance of phyla Euryarchaeota and Thaumarchaeota (Marine Group I) within our dataset. The distribution of different archaeal taxa and respective statistical analysis (SIMPER, NMDS) revealed a clear community shift along the sampling stations. The sampling stations (Godkhali and Bonnie camp) with history of higher hydrocarbon/oil pollution showed different archaeal community pattern (dominated by haloarchaea) compared to station (Dhulibhashani) with nearly pristine environment (dominated by methanogens). It is indicated that sediment archaeal community patterns were influenced by environmental conditions.read more
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Effective rhizoinoculation and biofilm formation by arsenic immobilizing halophilic plant growth promoting bacteria (PGPB) isolated from mangrove rhizosphere: A step towards arsenic rhizoremediation.
Ivy Mallick,Chandrima Bhattacharyya,Shayantan Mukherji,Dhritiman Dey,Somesh Chandra Sarkar,Ujjal Kumar Mukhopadhyay,Abhrajyoti Ghosh +6 more
TL;DR: Two As-resistant halophilic bacterial strains were isolated, identified and characterized from mangrove rhizosphere of Sundarban and when used as inoculum promoted the growth of rice seedlings but also decreased As uptake and accumulation in plants.
Journal ArticleDOI
Stratified Bacterial and Archaeal Community in Mangrove and Intertidal Wetland Mudflats Revealed by High Throughput 16S rRNA Gene Sequencing.
TL;DR: Seasonal variations of microbial communities within sediment profiles from two sediment types at three sampling locations in coastal Mai Po wetland were characterized using MiSeq high throughput sequencing and 16S rRNA quantitative PCR methods and showed clear decreasing trends with increasing depth.
Journal ArticleDOI
Comparative genomic inference suggests mixotrophic lifestyle for Thorarchaeota.
TL;DR: These genomes from deep anoxic layers suggest the presence of Thorarchaeota with the potential to degrade organic matter, fix inorganic carbon, reduce sulfur/sulfate and produce acetate, and may be involved in ethanol production, nitrogen fixation, nitrite reduction, and arsenic detoxification.
Journal ArticleDOI
Mangrove succession enriches the sediment microbial community in South China
TL;DR: Regression and ordination analyses indicated that the changes in the microbial community with mangrove succession were mainly associated with properties of the aboveground vegetation and the sediment and can probably be explained by increases in nutrients and microhabitat heterogeneity during mangroves succession.
Journal ArticleDOI
Comparative mangrove metagenome reveals global prevalence of heavy metals and antibiotic resistome across different ecosystems.
Madangchanok Imchen,Ranjith Kumavath,Debmalya Barh,Debmalya Barh,Aline B.M. Vaz,Aristóteles Góes-Neto,Sandeep Tiwari,Preetam Ghosh,Alice R. Wattam,Vasco Azevedo +9 more
TL;DR: Comparison of samples collected from a variety of habitats shows that genes involved in resistance to both heavy metals and antibiotics are ubiquitous, irrespective of the ecosystem examined.
References
More filters
Journal Article
R: A language and environment for statistical computing.
TL;DR: Copyright (©) 1999–2012 R Foundation for Statistical Computing; permission is granted to make and distribute verbatim copies of this manual provided the copyright notice and permission notice are preserved on all copies.
Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Methods of soil analysis.
Arnold Klute,Dwayne R. Buxton editor-in-chief Asa publications,John J. Mortvedt. editor-in-chief Sssa publications +2 more
Journal ArticleDOI
SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB
Elmar Pruesse,Christian Quast,Katrin Knittel,Bernhard M. Fuchs,Wolfgang Ludwig,Jörg Peplies,Frank Oliver Glöckner +6 more
TL;DR: SILVA (from Latin silva, forest), was implemented to provide a central comprehensive web resource for up to date, quality controlled databases of aligned rRNA sequences from the Bacteria, Archaea and Eukarya domains.
Journal ArticleDOI
Archaea in coastal marine environments.
TL;DR: Evidence for the widespread occurrence of unusual archaea in oxygenated coastal surface waters of North America is provided and it is suggested that these microorganisms represent undescribed physiological types of archaea, which reside and compete with aerobic, mesophilic eubacteria in marine coastal environments.
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