DREADDs for Neuroscientists.
TLDR
A primer on DREADDs is provided highlighting key technical and conceptual considerations and identify challenges for chemogenetics going forward.About:
This article is published in Neuron.The article was published on 2016-02-17 and is currently open access. It has received 1145 citations till now. The article focuses on the topics: Chemogenetics.read more
Citations
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Cell transplantation therapy for spinal cord injury
TL;DR: A better understanding of the mechanisms whereby these cells promote functional improvements will be important to make cell transplantation a viable clinical option and may lead to the development of more targeted therapies.
Journal ArticleDOI
Neural Circuit-Specialized Astrocytes: Transcriptomic, Proteomic, Morphological, and Functional Evidence
Hua Chai,Blanca Diaz-Castro,Eiji Shigetomi,Emma Monte,J. Christopher Octeau,Xinzhu Yu,Whitaker Cohn,Whitaker Cohn,Pradeep S. Rajendran,Thomas M. Vondriska,Julian P. Whitelegge,Julian P. Whitelegge,Giovanni Coppola,Giovanni Coppola,Baljit S. Khakh +14 more
TL;DR: In this article, the authors used multiple integrated approaches, including RNA sequencing (RNA-seq), mass spectrometry, electrophysiology, immunohistochemistry, serial block-face-scanning electron microscopy, morphological reconstructions, pharmacogenetics, and diffusible dye, calcium, and glutamate imaging, to directly compare adult striatal and hippocampal astrocytes under identical conditions.
Journal ArticleDOI
Mechanisms of signalling and biased agonism in G protein-coupled receptors
Denise Wootten,Arthur Christopoulos,Maria Marti-Solano,M. Madan Babu,Patrick M. Sexton,Patrick M. Sexton +5 more
TL;DR: Increasing molecular and structural understanding of biased agonism offers the possibility of designing improved GPCR-targeting drugs, and refined classification of drugs according to their pharmacodynamic profiles, which can be linked to receptor structure and predictions of preclinical drug efficacy.
Journal ArticleDOI
How Ligands Illuminate GPCR Molecular Pharmacology.
TL;DR: Structural and molecular insights into ligand engagement and action have enabled new computational methods and accelerated the discovery of novel ligands and tool compounds, especially for understudied andorphan GPCRs, and promise to streamline the development of GPCR-targeted medications.
Journal ArticleDOI
Astrocytic Activation Generates De Novo Neuronal Potentiation and Memory Enhancement.
Adar Adamsky,Adi Kol,Tirzah Kreisel,Adi Doron,Nofar Ozeri-Engelhard,Talia Melcer,Ron Refaeli,Henrike Horn,Limor Regev,Maya Groysman,Michael London,Inbal Goshen +11 more
TL;DR: It is discovered that astrocytic activation is not only necessary for synaptic plasticity, but also sufficient to induce NMDA-dependent de novo long-term potentiation in the hippocampus that persisted after astroCytic activation ceased.
References
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Søren G. F. Rasmussen,Brian T. DeVree,Yaozhong Zou,Andrew C. Kruse,Ka Young Chung,Tong Sun Kobilka,Foon Sun Thian,Pil Seok Chae,Els Pardon,Els Pardon,Diane M. Calinski,Jesper Mosolff Mathiesen,Syed T. A. Shah,Joseph A. Lyons,Martin Caffrey,Samuel H. Gellman,Jan Steyaert,Jan Steyaert,Georgios Skiniotis,William I. Weis,Roger K. Sunahara,Brian K. Kobilka +21 more
TL;DR: This crystal structure represents the first high-resolution view of transmembrane signalling by a GPCR and the most surprising observation is a major displacement of the α-helical domain of Gαs relative to the Ras-like GTPase domain.
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Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves.
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Evolving the lock to fit the key to create a family of G protein-coupled receptors potently activated by an inert ligand
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Predicting new molecular targets for known drugs
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