Journal ArticleDOI
Dynamic regulatory interactions of rad51, rad52, and replication protein-a in recombination intermediates.
Tomohiko Sugiyama,Noriko Kantake +1 more
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TLDR
These results suggest a regulatory role for Rad51 that suppresses ssDNA annealing and facilitates DNA strand invasion, where Rad51-double-stranded DNA may inhibit illegitimate second-end capture to ensure the error-free repair of a DNA double-strand break.About:
This article is published in Journal of Molecular Biology.The article was published on 2009-07-03. It has received 49 citations till now. The article focuses on the topics: Replication protein A & Strand invasion.read more
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Concentration-Dependent Exchange of Replication Protein a on Single-Stranded DNA Revealed by Single-Molecule Imaging
Bryan Gibb,Ling Feng Ye,Stephanie C. Gergoudis,Youngho Kwon,Hengyao Niu,Patrick Sung,Eric C. Greene +6 more
TL;DR: The results reveal an unanticipated exchange between bound and free RPA suggesting a binding mechanism that can confer exceptionally slow off rates, yet also enables rapid displacement through a direct exchange mechanism that is reliant upon the presence of free ssDNA-binding proteins in solution.
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Replication protein A: directing traffic at the intersection of replication and repair.
Greg G. Oakley,Steve M. Patrick +1 more
TL;DR: This review summarizes the current understanding of RPA structure, phosphorylation and protein-protein interactions in mediating these DNA metabolic processes.
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Human Rad52 binds and wraps single-stranded DNA and mediates annealing via two hRad52-ssDNA complexes
Jill M. Grimme,Jill M. Grimme,Masayoshi Honda,Rebecca Wright,Yusuke Okuno,Eli Rothenberg,Alexander V. Mazin,Taekjip Ha,Maria Spies,Maria Spies +9 more
TL;DR: In contrast to the wild type protein, hRad52RQK/AAA and hRad521–212 mutants with impaired ability to bind hR PA protein competed with hRPA for binding to ssDNA and failed to counteract hRpa-mediated duplex destabilization highlighting the importance of hRad 52-hRPA interactions in promoting efficient DNA annealing.
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DNA-PK, ATM and ATR collaboratively regulate p53-RPA interaction to facilitate homologous recombination DNA repair.
Moises A. Serrano,Zhengke Li,Mohan Dangeti,Phillip R. Musich,Steve M. Patrick,Marina Roginskaya,Brian M. Cartwright,Yue Zou +7 more
TL;DR: The results reveal a mechanism for the crosstalk between HR repair and NHEJ through the co-regulation of p53–RPA interaction by DNA-PK, ATM and ATR.
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Protein dynamics during presynaptic-complex assembly on individual single-stranded DNA molecules
TL;DR: This work illustrates the spatial and temporal progression of the association of RPA and Rad52 with the presynaptic complex and reveals a new RPA–Rad52–Rad51–ssDNA intermediate, with implications for how the activities of Rad52 and RPA are coordinated with Rad51 during the later stages of recombination.
References
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Journal ArticleDOI
Genetic analysis of yeast RPA1 reveals its multiple functions in DNA metabolism.
TL;DR: It is concluded that RPA1 has multiple roles in vivo and functions in DNA replication, repair, and recombination, like the single-stranded DNA-binding proteins of bacteria and phages.
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Efficient repair of HO-induced chromosomal breaks in Saccharomyces cerevisiae by recombination between flanking homologous sequences.
N Rudin,James E. Haber +1 more
TL;DR: The deletion-repair event provides an in vivo assay for the sensitivity of any particular recognition site to HO cleavage, and takes advantage of a galactose-inducible HO gene to follow the kinetics of this event at the DNA level and to search for intermediates in this reaction.
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Functional interactions among yeast Rad51 recombinase, Rad52 mediator, and replication protein A in DNA strand exchange.
Bin Wei Song,Patrick Sung +1 more
TL;DR: Rad51-catalyzed DNA strand exchange is greatly enhanced by the single-stranded (ss) DNA binding factor RPA if the latter is introduced after Rad51 has already nucleated onto the initiating ssDNA substrate, but does not appear to be more active than Rad51 when an RPA-free ssDNA template is used.
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Role of Saccharomyces Single-Stranded DNA-Binding Protein RPA in the Strand Invasion Step of Double-Strand Break Repair
Xuan Wang,James E. Haber +1 more
TL;DR: Chromatin immunoprecipitation techniques were used to visualize the kinetics and extent of RPA binding following induction of a double-strand break and during its repair by homologous recombination in yeast.
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Studies of the interaction between Rad52 protein and the yeast single-stranded DNA binding protein RPA.
TL;DR: It is demonstrated that a rad52 mutation is epistatic to the rfa1-44 mutation, placing RFA1 and RAD52 in the same genetic pathway, and a model incorporating the role of DNA in the protein-protein interactions involved in the repair of DNA double-strand breaks is presented.