Endothelial cells produce a latent inhibitor of plasminogen activators that can be activated by denaturants.
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Analysis of treated and untreated conditioned medium by gel filtration revealed that the latent and active PAIs migrated with apparent Mr values of 30,000 and 50,000, respectively, which indicate that the two PAIs are immunologically related and suggest thatThe latent form is converted into the active form by the sodium dodecyl sulfate present during the purification.About:
This article is published in Journal of Biological Chemistry.The article was published on 1985-09-25 and is currently open access. It has received 562 citations till now. The article focuses on the topics: Sodium dodecyl sulfate & Plasminogen activator.read more
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The serpin superfamily of proteinase inhibitors: structure, function, and regulation.
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Urokinase-type plasminogen activator: proenzyme, receptor, and inhibitors
TL;DR: Findings show that u-PA is synthesized and released from mammalian cells as a proenzyme with little or no activity (pro-u-PA), that cells possess a specific binding site for u- PA and pro-u -PA, and that they can also synthesize different rapidly acting, high affinity inhibitors of PAs.
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LDL receptor-related protein internalizes and degrades uPA-PAI-1 complexes and is essential for embryo implantation.
TL;DR: It is demonstrated that LRP mediates uptake and degradation of urokinase-type plasminogen activator-plasminogens activator inhibitor 1 complexes and proposed that the inability of the giant cells to remove the inactive protease complexes from their surfaces interferes with implantation of the embryo.
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Cytokine activation of vascular endothelium. Effects on tissue-type plasminogen activator and type 1 plasminogen activator inhibitor.
TL;DR: The fact that both rIL-1 beta and rTNF alpha act in a similar manner strengthens the hypothesis that the local development of inflammatory/immune processes could reduce endothelial fibrinolytic activity.
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Structural basis of latency in plasminogen activator inhibitor-1.
James Mottonen,Arne Strand,Jindrich Symersky,Robert M. Sweet,Dennis E. Danley,Kieran F. Geoghegan,Robert D. Gerard,Elizabeth J. Goldsmith +7 more
TL;DR: The structure of intact latent PAI-1 determined by single-crystal X-ray diffraction reveals that residues on the N-terminal side of the primary recognition site are inserted as a central strand of the largest βsheet, in positions similar to the corresponding residues in the cleaved form of the serpin α1proteinase inhibitor (α1-PI).
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril.
Pamela J. Fraker,John C. Speck +1 more
TL;DR: The stability and sparing solubility of this chloroglycoluril in water can account for the minimal damage to proteins and living cells observed in these iodinations and allow for elimination of the reduction step employed at the close of iodinations with soluble chloroamides such as chloramine-T.
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Plasminogen: Purification from Human Plasma by Affinity Chromatography
Dale G. Deutsch,Edwin T. Mertz +1 more
TL;DR: Plasminogen was prepared from human plasma by affinity chromatography on L-lysine-substituted Sepharose with a specific activity of 100 caseinolytic units per milligram of nitrogen.
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Synthesis of Antihemophilic Factor Antigen by Cultured Human Endothelial Cells
TL;DR: It is established that exogenous AHF procoagulant activity is not inactivated by the tissue culture system.