Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids
Ryo Nakabayashi,Keiko Yonekura-Sakakibara,Kaoru Urano,Makoto Suzuki,Yutaka Yamada,Tomoko Nishizawa,Fumio Matsuda,Mikiko Kojima,Hitoshi Sakakibara,Kazuo Shinozaki,Anthony J. Michael,Takayuki Tohge,Mami Yamazaki,Kazuki Saito +13 more
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TLDR
Antioxidative activity assays showed that anthocyanin overaccumulation with strong in vitro antioxidative activity mitigated the accumulation of reactive oxygen species in vivo under oxidative and drought stress, confirming the usefulness of flavonoids for enhancing both biotic and abiotic stress tolerance in crops.Abstract:
The notion that plants use specialized metabolism to protect against environmental stresses needs to be experimentally proven by addressing the question of whether stress tolerance by specialized metabolism is directly due to metabolites such as flavonoids. We report that flavonoids with radical scavenging activity mitigate against oxidative and drought stress in Arabidopsis thaliana. Metabolome and transcriptome profiling and experiments with oxidative and drought stress in wild-type, single overexpressors of MYB12/PFG1 (PRODUCTION OF FLAVONOL GLYCOSIDES1) or MYB75/PAP1 (PRODUCTION OF ANTHOCYANIN PIGMENT1), double overexpressors of MYB12 and PAP1, transparent testa4 (tt4) as a flavonoid-deficient mutant, and flavonoid-deficient MYB12 or PAP1 overexpressing lines (obtained by crossing tt4 and the individual MYB overexpressor) demonstrated that flavonoid overaccumulation was key to enhanced tolerance to such stresses. Antioxidative activity assays using 2,2-diphenyl-1-picrylhydrazyl, methyl viologen, and 3,3'-diaminobenzidine clearly showed that anthocyanin overaccumulation with strong in vitro antioxidative activity mitigated the accumulation of reactive oxygen species in vivo under oxidative and drought stress. These data confirm the usefulness of flavonoids for enhancing both biotic and abiotic stress tolerance in crops.read more
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Transcriptional control of flavonoid biosynthesis by MYB–bHLH–WDR complexes
TL;DR: Recent advances in the characterization of the underlying regulatory mechanisms of flavonoid biosynthesis are reviewed, with a special focus on the MBW (MYB-bHLH-WDR) protein complexes.
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Response of Phenylpropanoid Pathway and the Role of Polyphenols in Plants under Abiotic Stress.
TL;DR: The biochemical and molecular mechanisms related to the activation of phenylpropanoid metabolism are discussed and phenolic-mediated stress tolerance in plants is described to provide updated and brand-new information about the response of phenolics under a challenging environment.
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Plant Responses to Simultaneous Biotic and Abiotic Stress: Molecular Mechanisms
TL;DR: An insight is given into cross-tolerance between abiotic and biotic stress, focusing on the molecular level and regulatory pathways, which can lead to a cross-Tolerance and enhancement of a plant’s resistance against pathogens.
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Contribution of phenylpropanoid metabolism to plant development and plant-environment interactions.
TL;DR: Recent progress on elucidating the contribution of phenylpropanoid metabolism to the coordination of plant development and plant-environment interaction, and metabolic flux redirection among diverse metabolic routes are summarized.
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Plant MYB Transcription Factors: Their Role in Drought Response Mechanisms.
TL;DR: This review summarizes recent studies highlighting the role of the MYB family of transcription factors in the adaptive responses to drought stress and the practical application value of MYBs in crop improvement, such as stress tolerance engineering, is discussed.
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