Journal ArticleDOI
Exposure of fibrinogen-adherent platelets to plasma proteins: a new method for studying protein interactions with platelets.
Marita Broberg,Håkan Nygren +1 more
TLDR
Protein interactions with platelets may generate different results, depending on the mode of protein exposure, as shown in the results of this study.Abstract:
To understand the effects of mediators in coagulating blood at biomaterial surfaces, it is important to use methods that resemble the normal sequence of events in wound healing around implants. The initial adhesion of platelets from whole blood onto material surfaces is mediated by the fibrinogen receptor glycoprotein IIb/IIIa, as shown in a previous study (Broberg et al., J Lab Clin Med 2002; 139:163-172). In this study, isolated platelets were adhered to fibrinogen and exposed to IgG, von Willebrand factor, or thrombin. The response was detected as the number of adherent platelets, the spreading of platelets, the exposure of CD62P (P-selectin), and the release of platelet factor 4 (PF4), ADP, and ATP. These results were compared to the response of platelets adhering to surfaces coated with the same proteins. Fibrinogen-adherent platelets exposed to thrombin generated the significantly highest exposure of CD62P and release of PF4, ADP, and ATP. When platelets were adhered to different protein coatings, von Willebrand factor generated the most CD62P exposure, IgG generated the most PF4 release, and thrombin generated the highest concentration of ADP. These results indicate that protein interactions with platelets may generate different results, depending on the mode of protein exposure.read more
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Degradable, Multifunctional Cardiovascular Implants: Challenges and Hurdles
TL;DR: Degradable, multifunctional shape-memory polymers are introduced as a promising family of functional polymers that fulfill several requirements of modern implants and are of high relevance for cardiovascular application (e.g., stent technology).
Journal ArticleDOI
Cell mimetic lateral stabilization of outer cell mimetic bilayer on polymer surfaces by peptide bonding and their blood compatibility
K. Kaladhar,Chandra P. Sharma +1 more
TL;DR: The stability of the anchored bilayer under low shear stress conditions promises that the laterally stabilized supported bilayer system can be used for lowShear applications like small diameter vascular graft and modification of biosensors, and so forth.
Journal ArticleDOI
Activation of neutrophil granulocytes in an in vitro model of a cardiopulmonary bypass.
Ann E. Åsberg,Vibeke Videm +1 more
TL;DR: Neutrophil interaction with platelets and complement were more important for activation than biomaterial contact and use of the roller pump, and improvement of biocompatibility is dependent on modifying complement activation and platelet interaction with neutrophils.
Journal ArticleDOI
Inhibition of platelet receptors involved in neutrophil-platelet interaction in model cardiopulmonary bypass.
Ann E. Åsberg,Vibeke Videm +1 more
TL;DR: In conclusion, inhibiting platelet and neutrophil-platelet interactions had useful effects but no single blocking antibody seemed capable of inducing only beneficial effects.
References
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