Journal ArticleDOI
Fermentation optimization for the production of lovastatin by Aspergillus terreus: use of response surface methodology
J.L. Casas López,J.A. Sánchez Pérez,J. M. Fernández Sevilla,F.G. Acién Fernández,E. Molina Grima,Yusuf Chisti +5 more
TLDR
A Box-Behnken experimental design was used to investigate the effects of five factors (oxygen content in the gas phase, concentrations of C, N and P, and fermentation time) on the concentrations of biomass and lovastatin produced in batch cultures of Aspergillus terreus as discussed by the authors.Abstract:
A Box-Behnken experimental design was used to investigate the effects of five factors—ie oxygen content in the gas phase; concentrations of C, N and P; and fermentation time—on the concentrations of biomass and lovastatin produced in batch cultures of Aspergillus terreus. The values of the various factors in the experiment ranged widely, as follows: 20-80% (v/v) oxygen in the aeration gas; 8-48 g dm −3 C-concentration; 0.2-0.6 g dm −3 N-concentration; 0.5-2.5 g dm −3 phosphate-concentration; and 7-11 days fermentation time. No previous work has used statistical analysis in documenting the interactions between oxygen supply and nutrient concentrations in lovastatin production. The Box-Behnken design identified the oxygen content in the gas phase as the principal factor influencing the production of lovastatin. Both a limitation and excess of oxygen reduced lovastatin titers. A medium containing 48 g dm −3 C supplied as lactose, 0.46 g dm −3 N supplied as soybean meal, and 0.79 g dm −3 phosphate supplied as KH2PO4, was shown to support high titers (∼230 mg dm −3 )o f lovastatin in a7 -day fermentation in oxygen-rich conditions (80% v/v oxygen in the aeration gas). Under these conditions, the culture medium had excess carbon but limiting amounts of nitrogen. The optimized fermentation conditions raised the lovastatin titer by four-fold compared with the worst-case scenario within the range of factors investigated. 2004 Society of Chemical Industryread more
Citations
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Journal ArticleDOI
Optimization of date syrup as a novel medium for lovastatin production by Aspergillus terreus ATCC 20542 and analyzing assimilation kinetic of carbohydrates
TL;DR: Glucose and fructose assimilation kinetic parameters revealed that more lovastatin is produced during glucose assimilation, while more biomass was formed during fructose Assimilation.
Journal ArticleDOI
Development and validation of a reversed-phase HPLC method for simultaneous analysis of butylhydroxyanisol, simvastatin and its impurities in tablet dosage forms.
M. El Karbane,M. Azougagh,M Amood Al-Kamarany,H. Bouchafra,Yahia Cherrah,Abdelaziz Bouklouze +5 more
TL;DR: The described method was robust and successfully applied in quality control laboratories for routine analysis to determine the butylhydroxyanisol and simvastatin with its impurities content in tablet dosage forms.
Journal ArticleDOI
A Study of the Factors Effective in Morphogenesis of Aspergillus terreus in order to Increase the Production of Lovastatin
TL;DR: This study was conducted to analyze the factors effective in morphogenesis of this fungus to increase the industrial production of lovastatin, one of the most important types of statin drugs, commonly produced by Aspergillus terreus in a liquid culture.
Journal Article
A Novel Approach for Enhancement of Lovastatin Production using Aspergillus Species
TL;DR: Submerged cultures of filamentous fungi are widely used to produce commercially important metabolite lovastatin and Plackett-Burman experimental design was used for the first time to screen and investigate the effects of the nine factors, which identified the source and concentrations of C, N, pH and incubation period were the principal factor influencing the production of Lovastatin.
References
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George E. P. Box,D. W. Behnken +1 more
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