Journal ArticleDOI
Fermentation optimization for the production of lovastatin by Aspergillus terreus: use of response surface methodology
J.L. Casas López,J.A. Sánchez Pérez,J. M. Fernández Sevilla,F.G. Acién Fernández,E. Molina Grima,Yusuf Chisti +5 more
TLDR
A Box-Behnken experimental design was used to investigate the effects of five factors (oxygen content in the gas phase, concentrations of C, N and P, and fermentation time) on the concentrations of biomass and lovastatin produced in batch cultures of Aspergillus terreus as discussed by the authors.Abstract:
A Box-Behnken experimental design was used to investigate the effects of five factors—ie oxygen content in the gas phase; concentrations of C, N and P; and fermentation time—on the concentrations of biomass and lovastatin produced in batch cultures of Aspergillus terreus. The values of the various factors in the experiment ranged widely, as follows: 20-80% (v/v) oxygen in the aeration gas; 8-48 g dm −3 C-concentration; 0.2-0.6 g dm −3 N-concentration; 0.5-2.5 g dm −3 phosphate-concentration; and 7-11 days fermentation time. No previous work has used statistical analysis in documenting the interactions between oxygen supply and nutrient concentrations in lovastatin production. The Box-Behnken design identified the oxygen content in the gas phase as the principal factor influencing the production of lovastatin. Both a limitation and excess of oxygen reduced lovastatin titers. A medium containing 48 g dm −3 C supplied as lactose, 0.46 g dm −3 N supplied as soybean meal, and 0.79 g dm −3 phosphate supplied as KH2PO4, was shown to support high titers (∼230 mg dm −3 )o f lovastatin in a7 -day fermentation in oxygen-rich conditions (80% v/v oxygen in the aeration gas). Under these conditions, the culture medium had excess carbon but limiting amounts of nitrogen. The optimized fermentation conditions raised the lovastatin titer by four-fold compared with the worst-case scenario within the range of factors investigated. 2004 Society of Chemical Industryread more
Citations
More filters
Journal ArticleDOI
Aspergillus terreus Broth Rheology, Oxygen Transfer, and Lovastatin Production in a Gas-Agitated Slurry Reactor
E.M. Rodríguez Porcel,J.L. Casas López,J.A. Sánchez Pérez,J. M. Fernández Sevilla,J.L. García Sánchez,Yusuf Chisti +5 more
TL;DR: The non-Newtonian rheology of the fermentation broth was influenced both by the biomass concentration and the size of the fungal pellets, and the best biomass specific production of lovastatin was found.
Journal Article
A review on lovastatin and its production
TL;DR: Lovastatin application part is in various field like management of hypercholesterolaemia, cholesterol lowering actions, reduce the prevalence of Alzheimer’s and renal disease etc.
Journal ArticleDOI
A Falling‐Film Microreactor for Enzymatic Oxidation of Glucose
TL;DR: Two recent key technologies, microreactor technology and biotechnology, were combined to present an interesting alternative to conventional methods and open up excellent possibilities to intensify chemical processes in the field of fine chemicals.
Journal ArticleDOI
Spent sulphite liquor for cultivation of an edible rhizopus sp.
Jorge A. Ferreira,Patrik R. Lennartsson,Claes Niklasson,Magnus Lundin,Lars Edebo,Mohammad J. Taherzadeh +5 more
TL;DR: The fungal biomass produced from spent sulphite liquor is presently being tested as a replacement for fishmeal in feed for fish aquaculture and seems to be a potential source of nutrients and for production of glucosamine.
Journal ArticleDOI
Lovastatin production by Aspergillus terreus in a two‐staged feeding operation
TL;DR: The use of a fermentation strategy that continuously removes some of the lovastatin produced from the bioreactor can enhance its productivity by 315% compared with a conventional batch operation.
References
More filters
Journal ArticleDOI
Some New Three Level Designs for the Study of Quantitative Variables
George E. P. Box,D. W. Behnken +1 more
TL;DR: In this paper, a class of incomplete three level factorial designs useful for estimating the coefficients in a second degree graduating polynomial are described and the designs either meet, or approximately meet, the criterion of rotatability and for the most part can be orthogonally blocked.
Journal ArticleDOI
Mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent.
Alfred W. Alberts,Julie S. Chen,G Kuron,V Hunt,Jesse W. Huff,Carl H. Hoffman,John W. Rothrock,Lopez Maria B,Henry Joshua,Elbert E. Harris,Arthur A. Patchett,R L Monaghan,S Currie,E Stapley,George Albers-Schonberg,Otto D. Hensens,J Hirshfield,Karst Hoogsteen,Jerrold M. Liesch,James P. Springer +19 more
TL;DR: It was shown that mevinolin was an orally active cholesterol-lowering agent in the dog and orally administered sodium mevinolinate was an active inhibitor of cholesterol synthesis in an acute assay.
Journal ArticleDOI
Ml-236a, ml-236b, and ml-236c, new inhibitors of cholesterogenesis produced by penicillium citrinum
Journal ArticleDOI
Competitive inhibition of 3-hydroxy-3-methylglutaryl coenzyme a reductase by ML-236A and ML-236B fungal metabolites, having hypocholesterolemic activity
TL;DR: The experiments reported in this paper demonstrate that MG236A and ML-236B inhibit specifically 3-hydroxy-3-methylglutaryl (HMG)CoA reductase (EC 1 .I .1.34), the rate-limiting enzyme in cholesterol synthetic pathway, without affecting the rest of the enzymes involved in this pathway, and that the inhibition is competitive with respect to the substrate HMG-CoA.
Journal ArticleDOI
Secondary metabolites of the fungus Monascus : A review
TL;DR: This review deals with polyketides produced by the filamentous fungusMonascus which include: 1) a group of yellow, orange and red pigments, 2) agroup of antihypercholesterolemic agents including mevinolin and related compounds and 3) the newly discovered metabolite ankalactone.