Journal ArticleDOI
Fermentation optimization for the production of lovastatin by Aspergillus terreus: use of response surface methodology
J.L. Casas López,J.A. Sánchez Pérez,J. M. Fernández Sevilla,F.G. Acién Fernández,E. Molina Grima,Yusuf Chisti +5 more
Reads0
Chats0
TLDR
A Box-Behnken experimental design was used to investigate the effects of five factors (oxygen content in the gas phase, concentrations of C, N and P, and fermentation time) on the concentrations of biomass and lovastatin produced in batch cultures of Aspergillus terreus as discussed by the authors.Abstract:
A Box-Behnken experimental design was used to investigate the effects of five factors—ie oxygen content in the gas phase; concentrations of C, N and P; and fermentation time—on the concentrations of biomass and lovastatin produced in batch cultures of Aspergillus terreus. The values of the various factors in the experiment ranged widely, as follows: 20-80% (v/v) oxygen in the aeration gas; 8-48 g dm −3 C-concentration; 0.2-0.6 g dm −3 N-concentration; 0.5-2.5 g dm −3 phosphate-concentration; and 7-11 days fermentation time. No previous work has used statistical analysis in documenting the interactions between oxygen supply and nutrient concentrations in lovastatin production. The Box-Behnken design identified the oxygen content in the gas phase as the principal factor influencing the production of lovastatin. Both a limitation and excess of oxygen reduced lovastatin titers. A medium containing 48 g dm −3 C supplied as lactose, 0.46 g dm −3 N supplied as soybean meal, and 0.79 g dm −3 phosphate supplied as KH2PO4, was shown to support high titers (∼230 mg dm −3 )o f lovastatin in a7 -day fermentation in oxygen-rich conditions (80% v/v oxygen in the aeration gas). Under these conditions, the culture medium had excess carbon but limiting amounts of nitrogen. The optimized fermentation conditions raised the lovastatin titer by four-fold compared with the worst-case scenario within the range of factors investigated. 2004 Society of Chemical Industryread more
Citations
More filters
Journal ArticleDOI
Lovastatin production: From molecular basis to industrial process optimization.
Kelly C.L. Mulder,Flávia Furtado Mulinari,Octavio L. Franco,Maria S.F. Soares,Beatriz Simas Magalhães,Nádia Skorupa Parachin,Nádia Skorupa Parachin +6 more
TL;DR: This review brings, for the first time, complete information about the genetic basis for lovastatin production, detection and quantification, strain screening and cultivation process optimization, and all the information available from patent databases covering each protected aspect during Lovastatin bio-production.
Journal ArticleDOI
Studying pellet formation of a filamentous fungus Rhizopus oryzae to enhance organic acid production
Wei Liao,Yan Liu,Shulin Chen +2 more
TL;DR: It has been found that metal ions had significantly negative effects on pellet formation whereas soybean peptone had positive effects and PDB and calcium carbonate were beneficial to R. oryzae for growing small smooth pellets during the culture.
Journal ArticleDOI
Study of pellet formation of filamentous fungi Rhizopus oryzae using a multiple logistic regression model.
Yan Liu,Wei Liao,Shulin Chen +2 more
TL;DR: This paper conducted a comprehensive investigation using a completely randomized design (CRD) on a filamentous fungus, Rhizopus oryzae NRRL 395, in order to discover the effects of the above factors and establish a multiple logistic regression model to predict the probability of pellet formation.
Journal ArticleDOI
Response surface methodology for optimization of production of lovastatin by solid state fermentation
TL;DR: In this article, a 2(4) full-factorial central composite design (CCD) was chosen to explain the combined effects of the four medium constituents, viz. moisture content, particle size of the substrate, di -potassium hydrogen phosphate and trace ion solution concentration.
Journal ArticleDOI
Effects of the principal nutrients on lovastatin production by Monascus pilosus.
Tsuyoshi Miyake,Kumiko Uchitomi,Ming Yong Zhang,Isato Kono,Nobuyuki Nozaki,Hiroyuki Sammoto,Kenji Inagaki +6 more
TL;DR: Observations indicate that lovastatin production by M. pilosus is regulated by strict glucose repression and that an appropriate release from this repression by optimizing medium composition and/or by a mutation(s) is required for high lovASTatin production.
References
More filters
Journal ArticleDOI
Some New Three Level Designs for the Study of Quantitative Variables
George E. P. Box,D. W. Behnken +1 more
TL;DR: In this paper, a class of incomplete three level factorial designs useful for estimating the coefficients in a second degree graduating polynomial are described and the designs either meet, or approximately meet, the criterion of rotatability and for the most part can be orthogonally blocked.
Journal ArticleDOI
Mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent.
Alfred W. Alberts,Julie S. Chen,G Kuron,V Hunt,Jesse W. Huff,Carl H. Hoffman,John W. Rothrock,Lopez Maria B,Henry Joshua,Elbert E. Harris,Arthur A. Patchett,R L Monaghan,S Currie,E Stapley,George Albers-Schonberg,Otto D. Hensens,J Hirshfield,Karst Hoogsteen,Jerrold M. Liesch,James P. Springer +19 more
TL;DR: It was shown that mevinolin was an orally active cholesterol-lowering agent in the dog and orally administered sodium mevinolinate was an active inhibitor of cholesterol synthesis in an acute assay.
Journal ArticleDOI
Ml-236a, ml-236b, and ml-236c, new inhibitors of cholesterogenesis produced by penicillium citrinum
Journal ArticleDOI
Competitive inhibition of 3-hydroxy-3-methylglutaryl coenzyme a reductase by ML-236A and ML-236B fungal metabolites, having hypocholesterolemic activity
TL;DR: The experiments reported in this paper demonstrate that MG236A and ML-236B inhibit specifically 3-hydroxy-3-methylglutaryl (HMG)CoA reductase (EC 1 .I .1.34), the rate-limiting enzyme in cholesterol synthetic pathway, without affecting the rest of the enzymes involved in this pathway, and that the inhibition is competitive with respect to the substrate HMG-CoA.
Journal ArticleDOI
Secondary metabolites of the fungus Monascus : A review
TL;DR: This review deals with polyketides produced by the filamentous fungusMonascus which include: 1) a group of yellow, orange and red pigments, 2) agroup of antihypercholesterolemic agents including mevinolin and related compounds and 3) the newly discovered metabolite ankalactone.