Fermentative production and kinetics of cellulase protein on Trichoderma reesei using sugarcane bagasse and rice straw
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This is the first attempt of combining the synthetic substrate (xylose, lactose) with natural substrate (sugarcane bagasse, rice straw) and the mixture of substrates produced the highest maximal enzyme activity on cellulose with xylose.Abstract:
Cellulase a multienzyme made up of several proteins finds extensive applications in food, fermentation and textile industries. Trichoderma reesei is an efficient producer of cellulase protein. The comparative study was made on various carbon sources on the production of cellulase using strains of T. reesei QM 9414, 97.177 and Tm3. Pretreatment of sugarcane bagasse and rice straw offers very digestible cellulose and potentially less inhibition. Cellulase production was enhanced by multiple carbon sources because of diauxic pattern of utilization of substrates. This is the first attempt of combining the synthetic substrate (xylose, lactose) with natural substrate (sugarcane bagasse, rice straw). The mixture of substrates produced the highest maximal enzyme activity on cellulose with xylose, cellulose with lactose, bagasse with xylose, bagasse with lactose, rice straw with xylose and rice straw with lactose. In addition Monod growth kinetics and Leudeking piret product formation kinetics were studied using T. reesei with optimized medium under optimized conditions of inoculum concentration, D.O. level, agitator speed, temperature and pH.read more
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Culture-based strategies to enhance cellulase enzyme production from Trichoderma reesei RUT-C30 in bioreactor culture conditions
Aftab Ahamed,Patrick Vermette +1 more
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Production of Cellulases by Aspergillus Heteromorphus from Wheat Straw under Submerged Fermentation
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Bioprocessing of enhanced cellulase production from a mutant of Trichoderma asperellum RCK2011 and its application in hydrolysis of cellulose
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Secretome analysis of Trichoderma reesei and Aspergillus niger cultivated by submerged and sequential fermentation processes: Enzyme production for sugarcane bagasse hydrolysis.
Camila Florencio,F. M. Cunha,Alberto C. Badino,Cristiane S. Farinas,Eduardo Ximenes,Michael R. Ladisch +5 more
TL;DR: Proteomic analyses and enzymology studies are combined to show that sequential and submerged cultivation methods differently influence both titers and secretion profile of key enzymes required for the hydrolysis of sugarcane bagasse.
References
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Cellulase Production of Trichoderma reesei Rut C 30
TL;DR: In this paper, the cellulase production of a filamentous fungi, Trichoderma reesei Rut C 30, was examined on carbon sources obtained after steam pretreatment of spruce.
Book ChapterDOI
Cellulase production of Trichoderma reesei rut C 30 using steam-pretreated spruce
TL;DR: The conversion of cellulose using these enzymes was higher than that obtained with logen or Celluclast cellulases using steam-pretreated spruce as substrate, and the hydrolytic capacity of the produced enzyme solutions was higher compared with commercially available enzyme preparations.
Journal ArticleDOI
Studies on the production and application of cellulase from Trichoderma reesei QM-9414.
TL;DR: High yielding mutant strain, Trichoderma reesei QM-9414, was employed for the cellulase enzyme production and β-glucosidase supplementation was found to increase both the sugar yield and hydrolysis rate, and shorten the reaction time significantly.
Journal ArticleDOI
Morphology of Trichoderma reesei QM 9414 in submerged cultures
TL;DR: The microscopic morphology of Trichoderma reesei QM 9414, growing in submerged culture, was studied by image analysis and the distribution function with respect to the mycelia properties corresponds well with the experimental determination of the distributionfunction.
Journal ArticleDOI
Cellulases of Trichoderma reesei : influence of culture conditions upon the enzymatic profile
TL;DR: Culture conditions for which the FP/cellobiase ratio reaches the optimal values reported in the literature are determined and a well-balanced ratio was generally obtained with high C/N ratio in the medium and peanut cake as organic nitrogen source.