Fermentative production and kinetics of cellulase protein on Trichoderma reesei using sugarcane bagasse and rice straw
TLDR
This is the first attempt of combining the synthetic substrate (xylose, lactose) with natural substrate (sugarcane bagasse, rice straw) and the mixture of substrates produced the highest maximal enzyme activity on cellulose with xylose.Abstract:
Cellulase a multienzyme made up of several proteins finds extensive applications in food, fermentation and textile industries. Trichoderma reesei is an efficient producer of cellulase protein. The comparative study was made on various carbon sources on the production of cellulase using strains of T. reesei QM 9414, 97.177 and Tm3. Pretreatment of sugarcane bagasse and rice straw offers very digestible cellulose and potentially less inhibition. Cellulase production was enhanced by multiple carbon sources because of diauxic pattern of utilization of substrates. This is the first attempt of combining the synthetic substrate (xylose, lactose) with natural substrate (sugarcane bagasse, rice straw). The mixture of substrates produced the highest maximal enzyme activity on cellulose with xylose, cellulose with lactose, bagasse with xylose, bagasse with lactose, rice straw with xylose and rice straw with lactose. In addition Monod growth kinetics and Leudeking piret product formation kinetics were studied using T. reesei with optimized medium under optimized conditions of inoculum concentration, D.O. level, agitator speed, temperature and pH.read more
Citations
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Journal Article
Optimization of Cellulase Production By Aspergillus Flavus Nspr017 Cultured On Pre-Treated Agrowastes
TL;DR: Investigation of filamentous fungi for hyper-production of one of the most highly demanded industrial enzyme cellulase using cheap and readily available agro-wastes as the sole carbon sources under submerged fermentation found orange peels was the most effective at 5% concentration of all the agrowastes used.
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Efficiency of Some Biocides as Safe Alternatives to Fungicides on Cercospora Leaf Spot Control, their Effect on Biochemical Constituent and Yield of Sugar Beet
TL;DR: It was concluded that biocides, Bio-Zeid, Plant guard and Biobac could be sprayed six times/season at 15 days intervals as alternatives to fungicide applications to control Cercospora leaf spot of sugar beet and to produce higher yields of root and sugar with better quality.
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Investigation of the cellulases production by Aspergillus niger NSPR002 in different cultivation conditions.
TL;DR: Of all the fungal isolates screened, Aspergillus niger NSPR002 displayed the highest cellulases activity, and was found to be producers of cellulases with differences in the rate of biosynthesis.
Dissertation
Utilisation of cellulose waste for the production of a chemical intermediate of economic interest
TL;DR: In this article, a single-step glucose production process using Trichoderma reesei strain was proposed to reduce the use of pure enzymes and pre-treatments that are usually necessary to carry out the enzymatic degradation.
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Stimulatory Effect of Physicochemical Factors on the Expression of Cellulase by Trichoderma viride NSPRT23
TL;DR: Of all the selected fungal isolates screened, Trichoderma viride NSPRT23 was found to display highest cellulase activity and among tested carbon sources, pineapple peels at a concentration of 5%was found to be the best carbon source for cellulases production.
References
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Journal ArticleDOI
Metabolic engineering applications to renewable resource utilization.
Aristos Aristidou,Merja Penttilä +1 more
TL;DR: The conversion of both cellulose and hemicellulose for the production of fuel ethanol is being studied intensively, with a view to developing a technically and economically viable bioprocess.
Journal ArticleDOI
Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei.
TL;DR: RNA slot blot experiments showed that no expression could be observed on glucose-containing medium and that high glucose levels abolish the inducing effect of sophorose, showing that distinct and clear-cut mechanisms of induction and glucose repression regulate cellulase expression in an actively growing fungus.
Journal ArticleDOI
Regulation of gene expression in industrial fungi: Trichoderma.
Robert L. Mach,Susanne Zeilinger +1 more
TL;DR: This mini-review summarises the considerable amount of data accumulated over the past three decades regarding promoters of genes encoding hydrolytic enzymes, inducer molecules, and models for the recognition of the insoluble substrates cellulose and xylan.
Journal ArticleDOI
Influence of the carbon source on production of cellulases, hemicellulases and pectinases by Trichoderma reesei Rut C-30
TL;DR: The growth and enzyme production by Trichoderma reesei Rut C-30 using different lignocellulosic materials as carbon source were investigated and it was found that endoglucanase and endoxylanse activities were produced throughout the cultivations, whereas α-arabinosidase was induced late during the cultivation.
Journal ArticleDOI
Purification and characterization of five cellulases and one xylanase from Penicillium brasilianum IBT 20888
TL;DR: The filamentous fungus Penicillium brasilianum IBT 20888 was cultivated on a mixture of 30 g l−1 cellulose and 10 g xylan for 111 h and the resulting culture filtrate was used for protein purification and revealed similarity in molecular mass, pI and hydrolytic properties suggested that these two enzymes were identical, but the smaller was lacking the cellulose-binding domain or an essential part of it.