Fermentative production and kinetics of cellulase protein on Trichoderma reesei using sugarcane bagasse and rice straw
Reads0
Chats0
TLDR
This is the first attempt of combining the synthetic substrate (xylose, lactose) with natural substrate (sugarcane bagasse, rice straw) and the mixture of substrates produced the highest maximal enzyme activity on cellulose with xylose.Abstract:
Cellulase a multienzyme made up of several proteins finds extensive applications in food, fermentation and textile industries. Trichoderma reesei is an efficient producer of cellulase protein. The comparative study was made on various carbon sources on the production of cellulase using strains of T. reesei QM 9414, 97.177 and Tm3. Pretreatment of sugarcane bagasse and rice straw offers very digestible cellulose and potentially less inhibition. Cellulase production was enhanced by multiple carbon sources because of diauxic pattern of utilization of substrates. This is the first attempt of combining the synthetic substrate (xylose, lactose) with natural substrate (sugarcane bagasse, rice straw). The mixture of substrates produced the highest maximal enzyme activity on cellulose with xylose, cellulose with lactose, bagasse with xylose, bagasse with lactose, rice straw with xylose and rice straw with lactose. In addition Monod growth kinetics and Leudeking piret product formation kinetics were studied using T. reesei with optimized medium under optimized conditions of inoculum concentration, D.O. level, agitator speed, temperature and pH.read more
Citations
More filters
Book ChapterDOI
Bioethanol Production from Lignocellulosics: Some Process Considerations and Procedures
Charles Abbas,Wu Li Bao,Kyle E. Beery,Pamela Corrington,Consuelo Cruz,Lucas Loveless,Martin Sparks,Kelli Jean Trei +7 more
TL;DR: The main areas of research crucial to improved process economics consist of feedstock selection, pretreatment, hydrolysis of the pretreated feedstocks, and developing an optimized fermentation process that uses newly engineered ethanologens capable of utilizing various biomass-derived sugars.
Patent
Method for producing cellulase through solid state fermentation by adopting rice straws as main raw material
TL;DR: Wang et al. as mentioned in this paper provided a method for producing cellulase through solid state fermentation by adopting rice straws as a main raw material, wherein a strain Trichodema reesei QZ-5 preserved in the China Center for Type Culture Collection on June 5, 2012 and having a preservation number of CCTCC NO: M2012204 is adopted as a fermentation strain, agricultural product waste Rice straws are adopted as the primary raw material to carry out solid-state fermentation to prepare cellulase, the international standard method recommended by the International Union of Pure and
Review article cellulolytic microorganisms
TL;DR: Only a small percentage of microorganisms can degrade cellulose, probably because a wide range of cellulolytic organisms are not cultivable then unknown and not identified at present.
Book ChapterDOI
Influence of Significant Parameters on Cellulase Production by Solid-State Fermentation
TL;DR: In this paper, a book chapter focused on fungal cellulases in bioremediation and factors affecting cellulases production by solid-state fermentation (SSF) is presented.
References
More filters
Journal ArticleDOI
Metabolic engineering applications to renewable resource utilization.
Aristos Aristidou,Merja Penttilä +1 more
TL;DR: The conversion of both cellulose and hemicellulose for the production of fuel ethanol is being studied intensively, with a view to developing a technically and economically viable bioprocess.
Journal ArticleDOI
Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei.
TL;DR: RNA slot blot experiments showed that no expression could be observed on glucose-containing medium and that high glucose levels abolish the inducing effect of sophorose, showing that distinct and clear-cut mechanisms of induction and glucose repression regulate cellulase expression in an actively growing fungus.
Journal ArticleDOI
Regulation of gene expression in industrial fungi: Trichoderma.
Robert L. Mach,Susanne Zeilinger +1 more
TL;DR: This mini-review summarises the considerable amount of data accumulated over the past three decades regarding promoters of genes encoding hydrolytic enzymes, inducer molecules, and models for the recognition of the insoluble substrates cellulose and xylan.
Journal ArticleDOI
Influence of the carbon source on production of cellulases, hemicellulases and pectinases by Trichoderma reesei Rut C-30
TL;DR: The growth and enzyme production by Trichoderma reesei Rut C-30 using different lignocellulosic materials as carbon source were investigated and it was found that endoglucanase and endoxylanse activities were produced throughout the cultivations, whereas α-arabinosidase was induced late during the cultivation.
Journal ArticleDOI
Purification and characterization of five cellulases and one xylanase from Penicillium brasilianum IBT 20888
TL;DR: The filamentous fungus Penicillium brasilianum IBT 20888 was cultivated on a mixture of 30 g l−1 cellulose and 10 g xylan for 111 h and the resulting culture filtrate was used for protein purification and revealed similarity in molecular mass, pI and hydrolytic properties suggested that these two enzymes were identical, but the smaller was lacking the cellulose-binding domain or an essential part of it.