Journal ArticleDOI
Fluorophore tagged bio-molecules and their applications: A brief review
Ivy Sarkar,Ashok Kumar Mishra +1 more
Reads0
Chats0
TLDR
This review demonstrates the applications of such conjugated fluorescent molecular probes in different domains of biological activities and brings out the advantages and disadvantages of this particular type of fluorophores with the insight to the future perspectives.Abstract:
Bio-molecules are principal building blocks of living species and their inter-play is the cause of bio-activities. Various sophisticated experimental techniques as well as theoretical studies have ...read more
Citations
More filters
Topics In Fluorescence Spectroscopy
TL;DR: The topics in fluorescence spectroscopy is universally compatible with any devices to read, and is available in the digital library an online access to it is set as public so you can download it instantly.
Journal ArticleDOI
Spectroscopic methods for determination of critical micelle concentrations of surfactants; a comprehensive review
TL;DR: The applications of surfactants in various fields are gaining more attention, which makes full characterization of the surfactant characterization of growing interest as discussed by the authors, and it is fundamental to measure the critical micelle.
Journal ArticleDOI
A Systematic Study of Coumarin-Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging.
TL;DR: The best compound identified in this study showed excellent performance in live cell‐labeling experiments and enabled no‐wash fluorogenic imaging on a timescale of seconds.
Journal ArticleDOI
NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids.
Konstantin S. Mineev,Konstantin S. Mineev,Sergey A. Goncharuk,Sergey A. Goncharuk,Marina V. Goncharuk,Natalia V. Povarova,Anatolii I. Sokolov,Nadezhda S. Baleeva,Alexander Yu. Smirnov,Ivan N. Myasnyanko,Dmitry A. Ruchkin,Sergey Bukhdruker,Alina Remeeva,Alexey Mishin,Valentin Borshchevskiy,Valentin Borshchevskiy,Valentin Gordeliy,Alexander S. Arseniev,Dmitriy A. Gorbachev,Alexey S. Gavrikov,Alexander S. Mishin,Mikhail S. Baranov,Mikhail S. Baranov +22 more
TL;DR: The shortened FAST is designed, which is composed of only 98 amino acids, the shortest genetically encoded tag among all known fluorescent and fluorogen-activating proteins, by truncating 26 N-terminal residues.
Journal ArticleDOI
Tuning the Anti(myco)bacterial Activity of 3-Hydroxy-4-pyridinone Chelators through Fluorophores.
TL;DR: A critical account of the entire work in the design of chelators to address Mycobacterium avium infections is given and the statement “to label means to change” is justified.
References
More filters
Journal ArticleDOI
Molecular modeling of lipid probes and their influence on the membrane.
TL;DR: A number of Molecular Dynamics simulation studies are discussed which focus on the understanding of the behavior of lipid probes in biomembranes which are confined to the local neighborhood of the probe molecules which are sparsely used and generally present as single molecules.
Journal ArticleDOI
One-pot fluorescent labeling of saccharides with fluorescein-5-thiosemicarbazide for imaging polysaccharides transported in living cells.
TL;DR: Results indicate that the assay neither introduces discernible cytotoxicity against living cells nor obviously alters the functional activities of polysaccharides, and provides a convenient, highly efficient fluorescent labeling approach for imaging the transport of poly Saccharides in living cells.
Journal ArticleDOI
Transport of fluorescent bile acids by the isolated perfused rat liver: Kinetics, sequestration, and mobilization
Fernando Holzinger,Claudio D. Schteingart,H.-T. Ton-Nu,Carolina Cerrè,Joseph H. Steinbach,Hong-Zen Yeh,Alan F. Hofmann +6 more
TL;DR: It is concluded that fluorescent bile acids are efficiently transported across the basolateral membrane, but in contrast to natural conjugated bile acid, are sequestered in the hepatocyte (UDC derivatives > cholyl derivatives).
Journal ArticleDOI
The covalent attachment of multiple fluorophores to DNA containing phosphorothioate diesters results in highly sensitive detection of single-stranded DNA.
TL;DR: DNA fragments containing multiple internucleotidic phosphorothioate diesters, prepared by either chemical or enzymatic syntheses, are amenable to labeling with the fluorophore monobromobimane, and the detection limit for multiply labeled single-stranded DNA containing hundreds of base residues is in the low femtomole range.
Journal ArticleDOI
Resonance energy transfer imaging of phospholipid vesicle interaction with a planar phospholipid membrane: undulations and attachment sites in the region of calcium-mediated membrane--membrane adhesion.
TL;DR: The region of contact was detected as an area of planar membrane, coincident with the vesicle image, over which rhodamine fluorescence was sensitized by RET, and the total area of the contact region depended biphasically on the Ca2+ concentration, but the distance between the bilayers in this zone decreased with increasing [Ca2+].
Related Papers (5)
Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances.
Yoshio Suzuki,Kenji Yokoyama +1 more
Advances in the chemistry of small molecule fluorescent probes
Laura M. Wysocki,Luke D. Lavis +1 more