Journal ArticleDOI
Fluorophore tagged bio-molecules and their applications: A brief review
Ivy Sarkar,Ashok Kumar Mishra +1 more
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TLDR
This review demonstrates the applications of such conjugated fluorescent molecular probes in different domains of biological activities and brings out the advantages and disadvantages of this particular type of fluorophores with the insight to the future perspectives.Abstract:
Bio-molecules are principal building blocks of living species and their inter-play is the cause of bio-activities. Various sophisticated experimental techniques as well as theoretical studies have ...read more
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Topics In Fluorescence Spectroscopy
TL;DR: The topics in fluorescence spectroscopy is universally compatible with any devices to read, and is available in the digital library an online access to it is set as public so you can download it instantly.
Journal ArticleDOI
Spectroscopic methods for determination of critical micelle concentrations of surfactants; a comprehensive review
TL;DR: The applications of surfactants in various fields are gaining more attention, which makes full characterization of the surfactant characterization of growing interest as discussed by the authors, and it is fundamental to measure the critical micelle.
Journal ArticleDOI
A Systematic Study of Coumarin-Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging.
TL;DR: The best compound identified in this study showed excellent performance in live cell‐labeling experiments and enabled no‐wash fluorogenic imaging on a timescale of seconds.
Journal ArticleDOI
NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids.
Konstantin S. Mineev,Konstantin S. Mineev,Sergey A. Goncharuk,Sergey A. Goncharuk,Marina V. Goncharuk,Natalia V. Povarova,Anatolii I. Sokolov,Nadezhda S. Baleeva,Alexander Yu. Smirnov,Ivan N. Myasnyanko,Dmitry A. Ruchkin,Sergey Bukhdruker,Alina Remeeva,Alexey Mishin,Valentin Borshchevskiy,Valentin Borshchevskiy,Valentin Gordeliy,Alexander S. Arseniev,Dmitriy A. Gorbachev,Alexey S. Gavrikov,Alexander S. Mishin,Mikhail S. Baranov,Mikhail S. Baranov +22 more
TL;DR: The shortened FAST is designed, which is composed of only 98 amino acids, the shortest genetically encoded tag among all known fluorescent and fluorogen-activating proteins, by truncating 26 N-terminal residues.
Journal ArticleDOI
Tuning the Anti(myco)bacterial Activity of 3-Hydroxy-4-pyridinone Chelators through Fluorophores.
TL;DR: A critical account of the entire work in the design of chelators to address Mycobacterium avium infections is given and the statement “to label means to change” is justified.
References
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Aptamer-based optical probes with separated molecular recognition and signal transduction modules.
Na Li,Chih-Ming Ho +1 more
TL;DR: A general strategy was developed to transform aptamers into highly sensitive and selective optical sensors with an unmodified aptamer as the molecular recognition element and a competitor oligo, which was completely or partially complementary to the aptamer, as the signal transduction element.
Journal ArticleDOI
Probing the structure and function of the tachykinin neurokinin-2 receptor through biosynthetic incorporation of fluorescent amino acids at specific sites
Gerardo Turcatti,Karin Nemeth,Michael D. Edgerton,Ulrich Meseth,François Talabot,Manuel C. Peitsch,Jonathan Knowles,Horst Vogel,André Chollet +8 more
TL;DR: These data are the first report of the incorporation of a fluorescent unnatural amino acid into a membrane protein in intact cells by the method of nonsense codon suppression, as well as the first measurement of experimental distances between a G protein-coupled receptor and its ligand by FRET.
Journal ArticleDOI
Fluorescence measurements of benzene, naphthalene, anthracene, pyrene, fluoranthene, and benzo(e)pyrene in water.
Journal ArticleDOI
First Synthesis of Free Cholesterol−BODIPY Conjugates
TL;DR: An analogue of cholesterol and coprostanol containing the BODIPY fluorophore in the aliphatic tail of the free sterol exhibited a substantially similar physical behavior to cholesterol in model membranes with respect to localization in raft domains.
Journal ArticleDOI
Ligand binding to somatostatin receptors induces receptor-specific oligomer formation in live cells
Ramesh C. Patel,Ujendra Kumar,Don C. Lamb,John Eid,Magalie Rocheville,Michael P. Grant,Aruna Rani,Theodore L. Hazlett,Shutish C. Patel,Enrico Gratton,Yogesh C. Patel +10 more
TL;DR: It is proposed that induction by ligand of different oligomeric states of SSTRs represents a unique mechanism for generating signaling specificity not only within the SSTR family but more generally in the HHR family.
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