Journal ArticleDOI
Fluorophore tagged bio-molecules and their applications: A brief review
Ivy Sarkar,Ashok Kumar Mishra +1 more
TLDR
This review demonstrates the applications of such conjugated fluorescent molecular probes in different domains of biological activities and brings out the advantages and disadvantages of this particular type of fluorophores with the insight to the future perspectives.Abstract:
Bio-molecules are principal building blocks of living species and their inter-play is the cause of bio-activities. Various sophisticated experimental techniques as well as theoretical studies have ...read more
Citations
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Topics In Fluorescence Spectroscopy
TL;DR: The topics in fluorescence spectroscopy is universally compatible with any devices to read, and is available in the digital library an online access to it is set as public so you can download it instantly.
Journal ArticleDOI
Spectroscopic methods for determination of critical micelle concentrations of surfactants; a comprehensive review
TL;DR: The applications of surfactants in various fields are gaining more attention, which makes full characterization of the surfactant characterization of growing interest as discussed by the authors, and it is fundamental to measure the critical micelle.
Journal ArticleDOI
A Systematic Study of Coumarin-Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging.
TL;DR: The best compound identified in this study showed excellent performance in live cell‐labeling experiments and enabled no‐wash fluorogenic imaging on a timescale of seconds.
Journal ArticleDOI
NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids.
Konstantin S. Mineev,Konstantin S. Mineev,Sergey A. Goncharuk,Sergey A. Goncharuk,Marina V. Goncharuk,Natalia V. Povarova,Anatolii I. Sokolov,Nadezhda S. Baleeva,Alexander Yu. Smirnov,Ivan N. Myasnyanko,Dmitry A. Ruchkin,Sergey Bukhdruker,Alina Remeeva,Alexey Mishin,Valentin Borshchevskiy,Valentin Borshchevskiy,Valentin Gordeliy,Alexander S. Arseniev,Dmitriy A. Gorbachev,Alexey S. Gavrikov,Alexander S. Mishin,Mikhail S. Baranov,Mikhail S. Baranov +22 more
TL;DR: The shortened FAST is designed, which is composed of only 98 amino acids, the shortest genetically encoded tag among all known fluorescent and fluorogen-activating proteins, by truncating 26 N-terminal residues.
Journal ArticleDOI
Tuning the Anti(myco)bacterial Activity of 3-Hydroxy-4-pyridinone Chelators through Fluorophores.
TL;DR: A critical account of the entire work in the design of chelators to address Mycobacterium avium infections is given and the statement “to label means to change” is justified.
References
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Journal ArticleDOI
Cholesterol distribution in living cells: fluorescence imaging using dehydroergosterol as a fluorescent cholesterol analog.
TL;DR: It is shown that kinetic processes can be followed in living cells by monitoring recovery of DHE fluorescence in a photobleached region over time and provided evidence for the presence of a large intracellular cholesterol pool in the endocytic recycling compartment and the trans-Golgi network that might play important roles in the trafficking of lipids, lipid-anchored proteins, and transmembrane proteins that preferentially partition into cholesterol-enriched membrane domains.
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Cyclodextrins in delivery systems: Applications.
TL;DR: Current CD-based therapeutics is described and possible future applications are discussed, including the use of CD-containing polymers to provide unique capabilities for the delivery of nucleic acids.
Journal ArticleDOI
Orientation dependence in fluorescent energy transfer between Cy3 and Cy5 terminally attached to double-stranded nucleic acids
Asif Iqbal,Sinan Arslan,Burak Okumus,Timothy J. Wilson,Gerard Giraud,David Norman,Taekjip Ha,David M.J. Lilley +7 more
TL;DR: It is found that the efficiency of fluorescence resonance energy transfer between Cy3 and Cy5 terminally attached to the 5′ ends of a DNA duplex is significantly affected by the relative orientation of the two fluorophores.
Journal ArticleDOI
Light-switching excimer probes for rapid protein monitoring in complex biological fluids
TL;DR: Light-switching excimer aptamer probes for rapid and sensitive detection of a biomarker protein, platelet-derived growth factor (PDGF), were able to detect PDGF in a cell sample quantitatively without any sample pretreatment.
Journal ArticleDOI
Chemistry and biology of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-labeled lipids: fluorescent probes of biological and model membranes
TL;DR: Use of NBD-labeled lipids as analogues of natural lipids is critically evaluated and synthetic routes for preparing the lipids, and spectroscopic and ionization properties of these probes are reviewed in this report.
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