Journal ArticleDOI
Functional cDNA libraries from Drosophila embryos.
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TLDR
The design of the method and careful optimization of first strand synthesis have permitted cloning of several large cDNAs, and Transcription of essentially full-length clones with phage SP6 RNA polymerase produces RNAs that are efficiently translated in vitro to give complete, unfused products, thus permitting rapid characterization of the clones via the encoded polypeptides.About:
This article is published in Journal of Molecular Biology.The article was published on 1988-09-20. It has received 568 citations till now. The article focuses on the topics: Coding strand & Polymerase.read more
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LDL-receptor-related proteins in Wnt signal transduction
Keiko Tamai,Mikhall Semenov,Yolchi Kato,Rebecca Spokony,Chunming Liu,Yu Katsuyama,Fred Hess,Jean Pierre Saint-Jeannet,Xi He +8 more
TL;DR: In Xenopus embryos, LRP6 activated Wnt–Fz signalling, and induced Wnt responsive genes, dorsal axis duplication and neural crest formation, indicating that LRP 6 may be a component of the Wnt receptor complex.
Journal ArticleDOI
DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.
TL;DR: DMC1 phenotypes provide further evidence that recombination and SC formation are interrelated processes and are consistent with a requirement for DNA-DNA interactions during SC formation, and additional evidence suggests that arrest occurs at a meiosis-specific cell cycle "checkpoint" in response to a primary defect in prophase chromosome metabolism.
Journal ArticleDOI
A novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewal.
TL;DR: The Drosophila piwi gene is cloned and characterized and it is shown that it is required for the asymmetric division of GSCs to produce and maintain a daughter GSC but is not essential for the further differentiation of the committed daughter cell.
Journal ArticleDOI
arrow encodes an LDL-receptor-related protein essential for Wingless signalling
Marcel Wehrli,Scott T. Dougan,Kim A. Caldwell,Louise V. O'Keefe,Stephanie L. Schwartz,Stephanie L. Schwartz,Dalit Vaizel-Ohayon,Eyal D. Schejter,Andrew Tomlinson,Stephen DiNardo,Stephen DiNardo +10 more
TL;DR: It is shown that arrow gene function is essential in cells receiving Wingless input and that it acts upstream of Dishevelled, suggesting a new and conserved function for this LRP subfamily in Wingless/Wnt signal reception.
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Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea.
TL;DR: The results suggest that hydrophobins may have a role in the elaboration of infective structures by fungi and may fulfill other functions in fungal phytopathogenesis.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
Journal ArticleDOI
Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
Journal ArticleDOI
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
F W Studier,B A Moffatt +1 more
TL;DR: A gene expression system based on bacteriophage T7 RNA polymerase has been developed and high levels of accumulation suggest that the RNAs are relatively stable, perhaps in part because their great length and/or stem-and-loop structures at their 3' ends help to protect them against exonucleolytic degradation.
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A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback.
Diethard Tautz,Christine Pfeifle +1 more