Genetically Engineered Phages: a Review of Advances over the Last Decade
Diana Priscila Penso Pires,Diana Priscila Penso Pires,Sara Cleto,Sanna Sillankorva,Joana Azeredo,Timothy K. Lu +5 more
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TLDR
This review highlights advances in techniques used to engineer phages as vehicles for drug delivery and vaccines, as well as for the assembly of new materials, and discusses existing challenges and opportunities.Abstract:
Soon after their discovery in the early 20th century, bacteriophages were recognized to have great potential as antimicrobial agents, a potential that has yet to be fully realized. The nascent field of phage therapy was adversely affected by inadequately controlled trials and the discovery of antibiotics. Although the study of phages as anti-infective agents slowed, phages played an important role in the development of molecular biology. In recent years, the increase in multidrug-resistant bacteria has renewed interest in the use of phages as antimicrobial agents. With the wide array of possibilities offered by genetic engineering, these bacterial viruses are being modified to precisely control and detect bacteria and to serve as new sources of antibacterials. In applications that go beyond their antimicrobial activity, phages are also being developed as vehicles for drug delivery and vaccines, as well as for the assembly of new materials. This review highlights advances in techniques used to engineer phages for all of these purposes and discusses existing challenges and opportunities for future work.read more
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Antibiotic resistance in Pseudomonas aeruginosa: mechanisms and alternative therapeutic strategies
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Phage Therapy: A Renewed Approach to Combat Antibiotic-Resistant Bacteria.
TL;DR: How the modern scientific community works to improve the reliability and success ofphage therapy in the clinic is explored and how to properly evaluate the potential for phage therapy to combat antibiotic-resistant bacteria is discussed.
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Alternatives to Conventional Antibiotics in the Era of Antimicrobial Resistance
TL;DR: A review of the various promising approaches that have been adopted in the search for therapies that can substitute for antibiotics, including genetically modified phages, antibacterial oligonucleotides, and CRISPR-Cas9.
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Formation, physiology, ecology, evolution and clinical importance of bacterial persisters.
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References
More filters
Journal ArticleDOI
Bacterial biofilms : A common cause of persistent infections
TL;DR: Improvements in understanding of the genetic and molecular basis of bacterial community behavior point to therapeutic targets that may provide a means for the control of biofilm infections.
Journal ArticleDOI
CRISPR provides acquired resistance against viruses in prokaryotes
Rodolphe Barrangou,Christophe Fremaux,Hélène Deveau,Melissa Richards,Patrick Boyaval,Sylvain Moineau,Dennis A. Romero,Philippe Horvath +7 more
TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.
Journal ArticleDOI
Next-generation DNA sequencing.
Jay Shendure,Hanlee P. Ji +1 more
TL;DR: Next-generation DNA sequencing has the potential to dramatically accelerate biological and biomedical research, by enabling the comprehensive analysis of genomes, transcriptomes and interactomes to become inexpensive, routine and widespread, rather than requiring significant production-scale efforts.
Journal ArticleDOI
Origins and Evolution of Antibiotic Resistance
Julian Davies,Dorothy Davies +1 more
TL;DR: A review of antibiotic resistance development over the past half-century can be found in this article, with the oft-restated conclusion that it is time to act and to restore the therapeutic applications of antibiotics.
Journal ArticleDOI
Antibacterial resistance worldwide: causes, challenges and responses.
Stuart B. Levy,Bonnie Marshall +1 more
TL;DR: The optimism of the early period of antimicrobial discovery has been tempered by the emergence of bacterial strains with resistance to these therapeutics, and today, clinically important bacteria are characterized not only by single drug resistance but also by multiple antibiotic resistance.
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